Paper Details

Molecular analysis of copper resistance determinant (copA) in copper oxide nanoparticles resistant Pseudomonas fluorescens CuO-2 isolated from soil

Soltani Nezhad Shahla, Rabbani Khorasgani Mohammad, Emtiazi Giti, Yaghoobi Mohammad Mehdi, Shakeri Shahryar, Fanaei Maryam

DOI: https://dx.doi.org/10.12692/ijb/5.12.97-104

Int. J. Biosci. 5(12), 97-104. December, 2014. (PDF)

Abstract:

The pollution of the environment with toxic heavy metals is spreading and  to survive under metal-stressed conditions, bacteria have evolved several types of mechanisms.  In this study, copper resistance determinant (copA gene) in Pseudomonas fluorescens CuO-2 was investigated. The strain of P. fluorescens used in this study was previously isolated from soil. Genomic DNA was extracted from pure culture and the PCR method was used to investigate the copA gene. The copA gene that is responsible for resistance to copper was shown to be present in P. fluorescens CuO-2 by PCR. Nucleotide sequence of PCR fragment, demonstrated the presence of copA gene covering about approximately 300 bp involved in Cu resistance. The comparison of copA with other sequences in the genbank database demonstrated significant similarities to  copA  gene from other bacteria.  Comparative analysis of CopA protein from different species of Pseudomonas revealed that the copA gene product may plays a major role in the copper resistance mechanism.