Molecular assessment of hybridity in F1 (Luffa acutangula × Luffa cylindrica) through SSR marker
Paper Details
Molecular assessment of hybridity in F1 (Luffa acutangula × Luffa cylindrica) through SSR marker
Abstract
An experiment was carried out to test the hybridity of offspring of Luffa acutangula and Luffa cylindrica through SSR marker. Experiment was conducted at the laboratory of Department of Biotechnology, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh. Hybridity test through molecular maker is a new concept in the field of applied molecular biology in agriculture. The present finding indicated that SSR primer can be used for detection of polymorphism among the parents of any hybrid. The identified polymorphic primer can be used for hybrid identification for commercial purpose. All the seed companies producing and selling hybrid seeds can use this molecular technique. It will save money, time and manpower than traditional methods of hybridity test. Seven ridge gourd and three sponge gourd genotypes were used as parents. For hybridity test, initially different SSR primers were used to establish polymorphism among the parents of each hybrid. The identified polymorphic primers were used to screen the hybridity test. It was noticed that three primers viz. BoG-21, BoG-76 and BoG-117 showed polymorphism in the parents and their corresponding hybrid. It was noted that, a 550bp DNA fragment was amplified in male parent and hybrid of Bogra×RIG 47-2 which was absent in female genotype. 200 bp DNA fragment was amplified in male parent and hybrid in case of Bogra×BARI Jhinga-1. This DNA band can be used for large scale screening of that specific hybrid variety for seed purity in commercial purpose. The parents of one hybrid (Faridpur×KRG 02) showed monomorphic DNA band and one of the SSR primers viz. BoG 118 was not amplified in any ridge gourd and sponge gourd genotype under investigation. Hybridity (Luffa acutangula × Luffa cylindrica) was identified by the same DNA fragment that found in male parents and F1 offspring.
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