Molecular characteristic of bacteria associated with healthy Porites lutea coral of South Malang Waters, Indonesia

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Research Paper 01/12/2017
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Molecular characteristic of bacteria associated with healthy Porites lutea coral of South Malang Waters, Indonesia

Muhammad Arif Asadi, Bambang Semedi, Muliawati Handayani, Umi Zakiyah
J. Bio. Env. Sci.11( 6), 244-250, December 2017.
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Coral reefs are the most diverse of all marine ecosystem yet highly vulnerable to diseases and climate change impacts in which approximately 30% of corals have been affected globally. Porites lutea is among the most widespread coral in Indonesia, yet it is also highly impacted by the diseases. This study aimed to isolate, molecularly characterize and identify the associated bacteria that dominated the healthy P. lutea. The coral sampling was using snorkeling while streak method was used for bacterial isolation and purification. Molecular identification consisted of DNA extraction, 16S rRNA PCR amplification and sequencing of 16S rRNA gene flow, and BLAST homology. Results showed that the bacterium associated with healthy P. lutea was closely related to Marinobacter xestospongiae, Marinobacter zheijiangensis, and Marinobacter mobililis with a similarity of 96%, 96%, and 95% respectively. The bacterium can be used as a candidate for further anti-pathogenic bacterial test and may be able to inhibit the growth of pathogenic bacteria of coral diseases particularly Pink Line Syndrome that highly impact P. lutea in many areas.


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