Molecular characterization of cellulolytic nitrogen-fixing bacterial species isolated from Khyber Pakhtunkhwa
Paper Details
Molecular characterization of cellulolytic nitrogen-fixing bacterial species isolated from Khyber Pakhtunkhwa
Abstract
Bacteria inhabiting the soil rhizosphere had been recognized for their properties of promoting plant health. The current research was undertaken for the molecular characterization of cellulolytic nitrogen-fixing bacterial species from the rhizosphere. Two hundred and fifty soil samples were collected from Wheat and sugarcane rhizosphere in few districts of KPK and were grown on Nitrogen free Carboxymethyl cellulose medium for cellulose and nitrogenase enzyme production. Bacterial isolates from Wheat in Congo red assay showed clearance zone ratio on CMC substrate in the range of 1.6 – 3.0mm, while bacterial isolates from Sugarcane samples showed clearance zone ratio in the range of 1.3 – 2.71mm. Quantitative analysis revealed that wheat bacterial isolates were releasing cellulase in the range of 0.751– 4.784 U/mL on NF-CMC medium (with 5% CMC+pH 7) while they produced NH4-N in the range of 0.104 – 1.115 mM in NF medium (pH 7). Bacteria from Sugarcane samples showed cellulase activities in the range of 0.418 – 4.155 U/mL and produced NH4-N in the range of 0.102 – 1.070 mM under the same conditions. The presence of nitrogenase gene only in fourteen bacterial isolates was confirmed and identified by 16sRNA sequencing as bacterial strains Achromobacter sp. MFA1, Paenibacillus pabuli HSSC 492, Streptomyces sp. strain VITBVK2, Stenotrophomonas sp. Vi65, Uncultured Actinomyces sp. clone O-71, Beijerinckia fluminensis, Alcaligenes faecalis CCM5B, Stenotrophomonas maltophilia strain CM30, Brevibacillus parabrevis strain TJ2.3, Ochrobactrum intermedium strain BE1, Brevibacillus parabrevis strain TJ2.3, Cupriavidus sp. christensen.ICD.09 and Bacillus cereus.
Aatif A, Zakia L. 2011. Isolation and characterization of H2S producing yeast to detoxify mercury containing compounds. International Research Journal of Microbiology 2(12), 517-525.
Al-Khatib IA, Kontogianni S, Nabaa HA, Alshami N, Al-Sari MI. 2015. Public perception of hazardousness caused by current trends of municipal solid waste management. Waste Management 36, 323-330.
Bala Kumaran MD, Kalaichelvan PK, Santhi R. 2015. Exploration of Agro Industrial Wastes as Substrates for cellulose production by Bacillus licheniformis MTCC 429. Microbiology Journal 5(2), 36-42.
Boddey RM, Dobereiner J. 1995. Nitrogen fixation associated with grasses and cereals: recent progress and perspectives for the future. Fertility Research Journal 42, 241–250.
Callow N, Ray CS, Kelby MA, Ju LK. 2016. Nutrient control for stationary phase cellulase production in Trichoderma reesei Rut C-30. Enzyme and Microbial Technology 82, 8-14.
Cocking EC. 2003. Endophytic colonization of plant roots by nitrogen-fixing bacteria, Plant and Soil 252 (1), 169-175.
David BW. 2011. Microbial diversity of cellulose hydrolysis. Current Opinion in Microbiology 14(3), 259-263.
Emtiazi G, Pooyan M, Shamalnasab M. 2007. Cellulase Activities in Nitrogen Fixing Paenibacillus Isolated from Soil in N-free Media, World Journal of Agricultural Sciences 3(5), 602-608.
Farjana I, Narayan R. 2018. Screening, purification and characterization of cellulase from cellulase producing bacteria in molasses, Islam and Roy. BMC Research Note. 11, 445.
Franche C, Lindström K, Elmerich C. 2009. Nitrogen-fixing bacteria associated with leguminous and non-leguminous plants. Plant and Soil 321, 35-59.
Hassan M, Ali KW, Amin FR, Ahmad I, Khan MI, Abbas M. 2016. Economical perspective of sustainability in agriculture and environment to achieve Pakistan vision 2025. International. Journal of Agricultural and Environmental Research 2(2), 129-142.
Jonathan PZ, Bethany DJ, Steven MS, Greg FS. 2003. Nitrogenase gene diversity and microbial community structure: a cross system comparison. Environmental Microbiology 5(7), 539-554.
Kazaragis A. 2005. Minimization of atmosphere pollution by utilization of cellulose waste. Journal of Environmental Engineering and Landsciences 2, 81-90.
Krieg NR, Holt JG. 1984. Berjeys manual of systematic bacteriology, Ist edition.
Lester H, Sandra L. 1977. Solid Media Containing Carboxymethyl cellulose to Detect Cx Cellulase Activity of Micro-organisms. Journal of General Microbioloy 98, 109-1 I 5.
Lubanza N, Boipelo E, Olubukola OB. 2013. Isolation and characterization of beneficial indigenous endophytic bacteria for plant growth promoting activity in Molelwane Farm, Mafikeng, South Africa. African Journal of Biotech 12(26), 4105-4114.
Miller GL. 1959. Use of Dinitrosalisyclic acid reagent for determination of reducing sugar. Analytical Chemistry 31(3), 426-428.
Mohammad D, Sedigheh M, Saeed I. 2013. Identification of ten N2 fixing bacteria using 16sRNA and their response to various zinc concentration, International Journal of Cellular and Molecular Biotechnology, 1-8.
Pereira P, Ibanez F, Rosenblueth M, Etcheverry M, Martinez-Romero E. 2011. Analysis of the bacterial diversity associated with the roots of maize (Zea mays L.) through culture-dependent and culture-independent Methods. International Scholarly Research Notices. 1-10.
Pin-Jing H. 2012. Municipal solid waste in rural areas of developing country: do we need special treatment mode? Waste Management 32, 1289-1290.
Ponnambalam AS, Deepthi RS, Ghosh AR. 2011. Qualitative Display and Measurement of Enzyme Activity of Isolated Cellulolytic Bacteria, Journal of Biotechnology. Bioinformatics and Bioengineering 1(1), 33-37.
Reinhardt. 2008. Molecular characterization of nitrogen fixing bacteria isolated from Brazilian agricultural plants at Sao Paulo state. Brazilian Journal of Microbiology 39(3), 414-422.
San SY, Ei PK, Tin ML, Zaw KL, Amy Aung TM S, May Thet N, Wai WM. 2017. The correlation of carbon source and ammonium accumulation in culture broth by nitrogen fixing bacterial isolates. Journal of Scientific an Innovative Research 6(2), 63-67.
Sasidharan S, Sreedharan S, Sailas B. 2013. Cellulase Producing Bacteria from the Wood-Yards on Kallai River Bank. Advances in Microbiology 3, 326-332.
Shanmugapriya K, Saravana PS, Manoharan M, Mythili A, Joseph S. 2012. Isolation, screening and partial purification of cellulase from cellulase producing bacteria. International Journal of Advance Biotechnology Research 3, 509-514.
Yan-Ling L, Zheng Z, Min WU, Yuan W, Jia-Xun F. 2014. Isolation, Screening, and Identification of Cellulolytic Bacteria from Natural Reserves in the Subtropical Region of China and Optimization of Cellulase Production by Paenibacillus terrae ME27-1, Biomedical research international 1, 1-13.
Zaw K L, San san Y, Tin ML. 2013. Enhancement of Cellulolytic nitrogen fixing activity of Alcaligenes sp. By MNNG Mutagenesis. International Journal of Innovation and Applied Sciences 3(4), 979-986.
Zhou H, Long YQ, Meng AH, Li QH, Zhang YG. 2015. Classification of municipal solid waste components for thermal conversion in waste-to-energy research. Fuel 145, 151-157.
Nausheen Abdul Malik, Bashir Ahmad, Hira Mushtaq (2021), Molecular characterization of cellulolytic nitrogen-fixing bacterial species isolated from Khyber Pakhtunkhwa; IJB, V18, N3, March, P18-29
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