Paper Details

Molecular characterization of Nilssonia gangetica on the basis of rflp

Samah Bashir Kayani, Maqsood Anwar, Muhammad Ashfaq

DOI: https://dx.doi.org/10.12692/ijb/6.10.64-72

Int. J. Biosci. 6(10), 64-72. May, 2015. (PDF)

Abstract:

Nilssonia gangetica, a soft shell freshwater turtle, found in Rawalpindi – Islamabad area were identified and characterized on the basis of cytochrome b amplification and restriction fragment length polymorphism by using AluI, FokI, MspI, TaqI and HaeIII enzymes. Genetic diversity based on partial sequence of gene cytochrome b of Pakistani isolate was found to be varied from 8.7 to 50.2 % with highest difference with HE801750, HE801751 and AM495210, respectively. Ng|SamPk showed 100% genetic similarity based on nucleotide and amino acid sequences was found to be closely related to indian Nilssonia gangetica with Gen Bank accession No. EF558361. The results indicated the use of molecular techniques to be an efficient and more reliable to identify fresh water turtle species in general and endangered species in particular and to keep in their proper taxonomic position.