Molecular cloning and sequence analysis of a Terpene synthase (McTPS1) gene in Matricaria chamomilia

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Research Paper 01/07/2015
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Molecular cloning and sequence analysis of a Terpene synthase (McTPS1) gene in Matricaria chamomilia

Tingting Tao, Li Zhu, Xiaomeng Liu, Qiling Song, Feng Xu, Jie Chang, Weiwei Zhang
Int. J. Biosci.7( 1), 66-73, July 2015.
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A terpene synthase (TPS) gene (designated as McTPS1) cDNA was cloned from Matricaria chamomilia using a pair of specific primers. The cDNA fragment of McTPS1 was 1719 bp and encoded a protein of 572 amino acids. The theoretical molecular weight and isoelectric point of the deduced McTPS1 protein are 67 kDa and 5.27, respectively. Multiple alignments showed the amino acid sequence of McTPS1 have extensive homology with those of TPS proteins from other plant. Phylogenetic tree analysis revealed that McTPS1 had closer relationship with TPSs from Asteracae plants than from other plants. The molecular cloning and sequence analysis of McTPS1 gene enabled us to further understand the role of McTPS1 in the biosynthesis of α-bisabolol in M. chamomilia.


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