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Molecular detection and characterization of Staphylococcus aureus from buffalo milk from Muzaffargarh, Punjab, Pakistan

Research Paper | October 1, 2019

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Rehana Saleem, Muhammad Safdar, Rozhgar A. Khailany, Mehmet Ozaslan, Yasmeen Junejo, Belan O. Kanabe

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Int. J. Biosci.15( 4), 203-212, October 2019

DOI: http://dx.doi.org/10.12692/ijb/15.4.203-212


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This study was designed to identify and characterize Staphylococcus aureus (S. aureus) in the raw buffalos’ milk in district Muzaffargarh by microscopic, biochemical and molecular studies. Moreover, we evaluated different antibiotics on S. aureus to check the susceptibility of drug resistant bacteria, which were isolated from raw buffalos’ milk. A total of 100 milk samples were collected from different city markets of Jutoi, Alipur, Kotadu, Murad Abad, Choak Qureshi, Ahmadpur, Ruhilan Wali, shahjamal, Khanpur and Muzaffargarh city of district Muzaffargarh, Punjab, Pakistan. Milk samples were cultured onto various culture media for the isolation of bacteria. The isolated bacteria were identified by studying staining characteristics, cultural properties on different selective media, biochemical tests, catalas, coagulase, methyl red and voges prockauer, and finally by PCR. Out of 100 samples, 11 (11%) milk samples were found positive for S. aureus. 11 S. aureus isolates were amplified by 16S rRNA gene based PCR. Antimicrobial sensitivity test was carried out to ascertain the susceptibility of the organism to various antibiotics. This study was indicated the potential of staphylococcal food poisoning and health risks in district Muzaffargarh. Poor hygienic conditions effect the bacterial growth and S. aureus availability also related to the hygienic conditions.


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Molecular detection and characterization of Staphylococcus aureus from buffalo milk from Muzaffargarh, Punjab, Pakistan

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