Molecular detection of MERS-cor virus in the camels in different areas of Iraq

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Research Paper 01/01/2019
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Molecular detection of MERS-cor virus in the camels in different areas of Iraq

Karar Sabeeh Mahdi, Amin Al-Rawi
Int. J. Biosci.14( 1), 168-173, January 2019.
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Abstract

Middle East Respiratory Syndrome (MERS) is a zoonotic viral disease that can be transmitted from dromedaries to human beings. Therefore this study was conducted to investigate the prevalence of MERS-cor virus in the camels during the period from October 2017 to December 2018. A total of 70 nasal swap samples were collected randomly from three different areas in Iraq, (male and female) with age ranged from 14 days to 2 years. Nasal swap samples were divided into identical triazole and transport media for viral RNA extraction and cultivation of the suspected cases for Mers-co virus isolation in embryonic tissue culture cells. The extracted RNA was tested by Nano drop to check RNA concentration and estimation of RNA purity. Cultivation of the samples in embrocated tissue culture cells showed visible CPE after 48 hr. Detection of the Mers-cor virus by real-time PCR showed that two males and 2 females from Najaf and Waist governorate were infected with the virus. In conclusion; Detection of Mers-co virus indicates the importance of the controlling its spread. The propagation and adaptation of the virus to grow and isolated in chicken embryo fibroblast cell culture as the first time work in Iraq is recommended instead of propagation of the virus cell line tissue culture which is not available and not applicable in Iraq.

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