Paper Details

Molecular discrimination of the advanced breeding lines of durum wheat (Triticum turgidum L.) based on inter simple sequence repeat markers

Laya Azizi,  Reza Mohammadi, Alireza Etminan, Lia Shooshtari

DOI: https://dx.doi.org/10.12692/ijb/5.12.130-135

Int. J. Biosci. 5(12), 130-135. December, 2014. (PDF)

Abstract:

Durum wheat (Triticum turgidum  L. var. durum) is the most suitable wheat to produce pasta and spaghetti because of its high protein content. A set of 14 durum breeding lines along with three check cultivars (i.e., Saji, Zardak and Sardari), 10 ISSR primers were used to estimate genetic diversity among genotypes. The primers produced 111 bands, among which 85 bands (about 76.5%) were polymorphic. A total of 85 polymorphic fragments were scored with average 11.1 polymorphic bands per primer. The average of PIC index was 0.33, that showed a good efficiency of primers to separate the genotypes. Cluster analysis using UPGMA method and Dice similarity coefficient categorized the genotypes into five main groups in which the check genotypes were classified in the separated groups.