Molecular genetic relationships among Haloxylon salicornicum plant and its closely related taxa in Saudi Arabia

Paper Details

Research Paper 01/02/2017
Views (290) Download (18)

Molecular genetic relationships among Haloxylon salicornicum plant and its closely related taxa in Saudi Arabia

Sanad M. Al Sobeai
Int. J. Biosci.10( 2), 81-88, February 2017.
Certificate: IJB 2017 [Generate Certificate]


The present study encompasses molecular identification, genetic distance and genetic relationships among Haloxylon salicornicum plants collected from the Saudi Arabia and its closely related taxa was investigated by sequencing the amplified ITS region including the 3` end 40 bp from 18S gene, the complete ITS1, 5.8S rDNA and ITS2 as well as 104 bp from the 5` end of 26S gene. The results showed that the H. salicornicum of Saudi Arabia desert has low level of genetic distance and genetic diversity when compared with its closely related taxa. These high level of genetic similarities of H. salicornicum and its closely related taxa might be due to the controlled gene flow and small size of samples. Further studies at the molecular level are also required to elucidate and provide intimations for more understand the interplay of genetic distance and genetic relationships among H. salicornicum with closely associated plants.


Abbas S, Farhatullah J, Marwat KB, Khan IA. Munir I. 2009. Molecular analysis of genetic diversity in Brassica species. Pak. J. Bot 41, 167-176.

Abdallah EM, El-Ghazali GE. 2013. Screening for antimicrobial activity of some plants from Saudi folk medicine. Glob J Res Med Plants Indigen Med 2, 189-197.

Ahmad M, Eram S. 2011. Hepatoprotective studies on Haloxylon salicornicum: a plant from Cholistan Desert. Pak J Pharm Sci 24, 377-382.

Ajabnoor MA, Al-Yahya MA, Tariq M, Jayyab AA. 1984. Antidiabetic activity of Hammada salicornica,” Fitoterapia, vol. 55, no. 2, pp. 107-109.

Akhani, Hossein, Edward, Gerald, Roalson, Eric H. 2007. Diversification of the Old World Salsoleae S.L. (Chenopodiaceae): Molecular Phylogenetic Analysis of Nuclear and Chloroplast Data Sets and a Revised Classification”. International Journal of Plant Sciences 168(6), 931-956.

Ali W, Munir I,  Ahmad, MA, Muhammad W, Ahmed, Durrishahwar N,  Ali S.  Swati ZA, 2007. Molecular characterization of some local and exotic Brassica juncea germplasm. Afr. J. Biotechnol 6, 1634-1638.

AL-Sobeai, Sanad M, Alamer, Khalid H, Amer, Sayed AM. 2015. A preliminary molecular variability within Haloxylon salicornium accessions growing in Saudi Arabia . Plant Omics, Vol. 8, No. 6, 604-608.

Ashraf MA, Karamat M, Shahnaz K, Abdul W, Ismail Y. 2012 . Study of chemical and mineral constituents of Haloxylon salicornicum collected from Cholistan Desert, Bahawalpur, Pakistan,” Wlfenia Journal, vol. 19, no. 10, pp. 306-327.

Baldwin BG, Sanderson MJ, Porter JM, Wojciechowski MF, Campbell CS, Donoghue MJ. 1995. The ITS region of nuclear ribosomal DNA: a valuable source of evidence on angiosperm phylogeny. Ann. Missouri. Bot. Gard 82, 247-277.

Boulos L. 1999. Flora of Egypt, vol. 1, Al-Hadra, Cairo, Egypt.

Brown G, Al-Mazrooei S. 2001. Germination ecology of Haloxylon salicornicum from Kuwait. Bot Jahrb Syst Pflanzengesch Pflanzengeogr 123, 235-247.

Dagla HR, Shekhawat NS. 2005. In vitro multiplication of Haloxylon recurvum (Moq.)- a plant for saline soil reclamation, J Plant Biol 7(3), 155-160.

El-Shazly AM, Dora G, Wink M. 2005. Alkaloids of Haloxylon salicornicum (Moq.) Bunge ex Boiss. (Chenopodiaceae),” Pharmazie, vol 60, no. 12, pp. 949-952.

Ghazali GE, Khalifa S, Gameel AS, Abdallah EM. 2010. Traditional medicinal plants indigenous to Al-Rass province, Saudi Arabia. J Med Plant Res        4, 2680-2683.

Gibbons S, Denny BJ, Ali-Amine S, et al. 2000. NMR spectroscopy, X-ray crystallographic, and molecular modeling studies on a new pyranone from Haloxylon salicornicum,” Journal of Natural Products, vol 63, no. 6, pp. 839-840.

Hedge IC. 1997. “Haloxylon”. In Rechinger, Karl Heinz et al. Flora Iranica Bd. 172, Chenopodiaceae. Graz: Akad. Druck. pp. 315-326.

Leegesse BW, Myburg AA, Pixley KV, Botha M.  2007. Genetic diversity of African maize inbred lines revealed by SSR markers. Hereditas 144, 10-17.

Omar S, Al-Mutawa Y, Zaman S. 2000. Vegetation of Kuwait. Kuwait Institute for Scientic research, Al-Assriya Printing press, publishing and distribution company, Kuwait pp. 159.

Pyankov VI, Artyusheva EG, Edwards GE, Black CC, JR, Soltis PS. 2001 Phylogenetic analysis of tribe Salsoleae (Chenopodiaceae) based on ribosomal ITS sequences: Implications for the evolution of photosynthesis types. Am. J. Bot 88(7), 1189-1198.

Saleem N. 2012. An ethno-pharmacological study of Egyptian Bedouin women’s knowledge of medicinal plants. 2015. Ph.D. Thesis, University of Strathclyde, Glasgow UK.

Singh JP, Rathore VS, Roy MM. 2015. Notes about Haloxylon salicornicum (Moq.) Bunge ex Boiss., a promising shrub for arid regions. Genet Resour Crop Evol 62, 451-463.

Soliman GA, Donia Ael R, Awaad AS, Algasoumi SI, Yusufoglu H. 2012. Effects of Emex spinosa, Leptadeniapyro technica, Haloxylon salicornicum and Ochradenus baccatus extracts on the reproductive organs of male rats. Pharm Biol 50,105-112.

Tackholm V. 1974. Students’ Flora of Egypt, Cairo University Press, Cairo, Egypt, 2nd edition.

Tang RG, Gao L, He Z, Han S, Shan, Zhong R, Zhou C, Jiang J, Li Y. Zhuang W. 2007. Genetic diversity in cultivated groundnut based on SSR markers. J. Genet. Genom 34, 449-459.

Wang HY, Wei YM, Yan ZH, Zheng YL. 2007. EST-SSR DNA polymorphism in durum wheat (Triticum durum L.) collections. J. Appl. Genet 48, 35-42.

Yokoo Y, Kohda H, Kusumoto A, et al. 1999. Isolation from beer and structural determination of a potent stimulant of gastrin release,” Alcohol and Alcoholism, vol 34, no. 2, pp. 161-168.

Zaman S, Padmesh S, Bhat NR, Tawfiq H. 2006. Germination of some Kuwait’s native plants under saline conditions. Am Eurasian J Agric Environ Sci 1(2), 146-148.