Molecular genotyping of methicillin-resistant Staphylococcus aureus by employing DNA marker technology
Paper Details
Molecular genotyping of methicillin-resistant Staphylococcus aureus by employing DNA marker technology
Abstract
Staphylococcus aureus are Gram positive, non-motile and facultative anaerobic coccus often golden yellow cells with pigments and usually arranged in clusters like grapes. Methicillin-Resistant Staphylococcus aureus (MRSA) are S. aureus strains that are resistant to methicillin and many other antibiotics. The present study aimed to molecular characterizes the 23 MRSA isolates obtained from effected patients and characterized by Randomly Amplified Polymorphic DNA (RAPD). Total of 14 RAPD primers amplified 827 fragments with range of 3 to 8 and level of polymorphism was 93.9%. Genetic similarities range is 88.73% to 52.11%, maximum genetic similarity was found between strain number 143 and 136 while the minimum genetic similarity was found among strain number 141 and 88. Genetic relationship among the MRSA isolates was analyzed through cluster analysis. All isolates were clustered in three major groups and maximum isolates were present in the second group. The present findings could be of beneficial use in scheming the sources as well as modes of transmission, trailing the spread of strains within and between hospitals, and mainly inhibits the nosocomial infections that are caused by the MRSA.
Babalola OO. 2003. Molecular techniques: An overview of methods for the detection of bacteria. African journal of biotechnology 2(12), 710-3.
Chambers HF, DeLeo FR. 2009. Waves of resistance: Staphylococcus aureus in the antibiotic era. Nature Review Microbiol 7, 629-641.
DeLeo FR, Otto M, Kreiswirth BN, Chambers HF. 2010. Community associated meticillin-resistant Staphylococcus aureus. The Lancet 7; 75(97), 1557-68.
Gordon RJ, Lowy FD. 2008. Pathogenesis of methicillin-resistant Staphylococcus aureus infection. Clinical infectious diseases 1, 46(Supplement 5): S350-9.
Karakulska J, Pobucewicz A, Nawrotek P, Muszyńska M, Furowicz A, Czernomysy-Furowicz D. Molecular typing of Staphylococcus aureus based on PCR-RFLP of coa gene and RAPD analysis. Polish journal of veterinary sciences. 2011 May 1; 14(2), 285-6.
Kennedy AD, DeLeo FR. 2009. Epidemiology and virulence of community associated MRSA. Clinical Microbiology Newsletter 15; 31(20), 153-60.
Lee YT, Lin DB, Wang WY, Tsao SM, Yu SF, Wei MJ, Yang SF, Lu MC, Chiou HL, Chen SC, Lee MC. 2011. First identification of methicillin-resistant Staphylococcus aureus MLST types ST5 and ST45 and SCCmec types IV and Vt by multiplex PCR during an outbreak in a respiratory care ward in central Taiwan. Diagnostic microbiology and infectious disease 30; 70(2), 175-82.
Lee YT, Lin DB, Wang WY, Tsao SM, Yu SF, Wei MJ, Yang SF, Lu MC, Chiou HL, Chen SC, Lee MC. 2011. First identification of methicillin-resistant Staphylococcus aureus MLST types ST5 and ST 45 and SCCmec types IV and Vt by multiplex PCR during an outbreak in a respiratory care ward in central Taiwan. Diagnostic microbiology and infectious disease 30; 70(2), 175-82.
Manakant I, Tanaya K. 2012. Antibiotic resistance and RAPD-PCR typing of multidrug resistant MRSA isolated from bovine mastitis cases in Thailand. Journal of science Asia 38, 30-35
Neela V, Mariana NS, Radu S, Zamberi S, Raha AR, Rosli R. 2005. Use of RAPD to investigate the epidemiology of Staphylococcus aureus infection in Malaysian hospitals. World Journal of Microbiology and Biotechnology 1; 21(3), 245-51.
Neslihan I, Bilkay, Isil Seyis. 2014. Application of RAPD-PCR for Determining the Clonality of Methicillin Resistant Staphylococcus aureus Isolated from Different Hospitals. Brazilian Archives of Biology and Technology 57(4), 548-553.
Nikbakht M, Nahaei MR, Akhi MT, Asgharzadeh M, Nikvash S. 2008. Molecular fingerprinting of meticillin-resistant Staphylococcus aureus strains isolated from patients and staff of two Iranian hospitals. Journal of Hospital infection 69(1), 46-55.
Otter JA, French GL. 2010. Molecular epidemiology of community associated meticillin-resistant Staphylococcus aureus in Europe. The Lancet infectious diseases 10(4), 227-39.
Plata K, Rosato AE, Wegrzyn G. 2009 Staphylococcus aureus as an infectious agent: overview of biochemistry and molecular genetics of its pathogenicity. Acta Biochimica Polonica. Nov 56(4), 597.
Power EG. 1996. RAPD typing in microbiology—a technical review. Journal of Hospital Infection 34(4), 247-65.
Naima Mahreen, Aasma Akhtar, Javaria Altaf, Muhammad Saqib, Mian Abdul Hafeez, Faisal Saeed Awan, 2017. Molecular genotyping of methicillin-resistant Staphylococcus aureus by employing DNA marker technology. J. Biodiv. Environ. Sci., 10(6), 330-335.
Copyright © 2017 by the Authors. This article is an open access article and distributed under the terms and conditions of the Creative Commons Attribution 4.0 (CC BY 4.0) license.