Molecular identification of Bacteria causing soft rot disease of tomato in Mansehra, Pakistan

Paper Details

Research Paper 01/08/2020
Views (333) Download (20)

Molecular identification of Bacteria causing soft rot disease of tomato in Mansehra, Pakistan

Naseeb Ullah, Aziz Ud-Din, Tariq Mahmood, Hamid Ali, Wajid Ali, Sajidul Ghafoor, Shakir Khan, Safa Faqir, Raja Asad Ali Khan
Int. J. Biosci.17( 2), 207-217, August 2020.
Certificate: IJB 2020 [Generate Certificate]


Vegetables are regarded as the prolonged plant parts which are used either cooked or uncooked. Different vegetables have different nutritional values providing vitamins, antioxidant, minerals, fibers for the normal functioning of the human body. Pakistan is an agriculture country and above 70% of its population depends on agriculture. Mansehra is the North-West district of the province Khyber Pakhtunkhwa, Pakistan. It is famous for tomato (Lycopersicum esculentum) cultivation, However, there are certain limiting factors which lower the yield of tomato. Besides others the most important yield limiting factor is diseases caused by bacteria. These diseases pose a serious threat to the quality and quantity of tomato. We collected symptomatic samples of tomato showing symptoms of soft rot in the year 2016-2017. Bacteria were isolated and were further cultured to get pure colonies. We applied biochemical tests and molecular markers for the identification of isolated bacteria. The isolated bacteria were further subjected to PCR and we sequenced partially their 16S rRNA. It was revealed that Erwinia and pseudomonas were the causing agents. The obtained sequences of 16S rRNA were submitted to NCBI gene bank having accession numbers MH244345.1 and MH244346.1


AGRIOS GN. 1997. Plant diseases caused by prokaryotes: bacteria and mollicutes. Plant pathology 4, 407-470.

Ali F, Parikh A, Shah M. 1994. Measurement of profit efficiency using behavioural and stochastic frontier approaches. Applied Economics 26, 181-188.

De Boer S, Ward L. 1995. PCR detection of Erwinia carotovora subsp. atroseptica associated with potato tissue. Phytopathology 85, 854-858.

El‐Hendawy H, Osman M, Ramadan H. 2002. Pectic enzymes produced in vitro and in vivo by Erwinia spp. isolated from carrot and pepper in Egypt. Journal of Phytopathology 150, 431-438.

Farrar J, Nunez J, Davis R. 2000. Influence of soil saturation and temperature on Erwinia chrysanthemi soft rot of carrot. Plant disease 84, 665-668.

Hannukkala A, Segerstedt M. 2004. Epidemiology, diagnostics and control of blackleg and soft rot on potato Maa-ja elintarviketalous 41. 60 pp.

Iftikhar S, Ahmad I, Soomro M, Aslam M. 1993. Powdery and common scab in spring potato crop in irrigated plains of Central Punjab and Pabbi area of North West Frontier Province (NWFP), Pakistan.

International Commission of Microbiological Specification for Foods. 1998. Microorganisms in Food, Microbial Ecology of Food Commodities. New York Blackie Academic and Professional. Vol 6, 617-620.

Jabuonski R, Takatsu A, Reifschneider F. 1986. Avaliação da patogenicidade de bactérias do gênero Erwinia isoladas de batateira, tomateiro e de outras plantas hospedeiras. Fitopatologia Brasileira 11, 587.

Khan AB, Panhwar WA. 2017. Population of Thrips tabaci Lindeman, 1889 in Onion Crop from district Mansehra, Khyber Pakhtunkhwa, Pakistan.

Lelliott R, Dickey S. 1984. Erwinia Winslow et al. Bergey’s Manual of Systematic Bacteriology (NR Krieg, and JG Holt, eds.) 1, 469-476.

Lohano HD, Mari FM. 2005. Spatial price linkages in regional onion markets of Pakistan. Journal of Agriculture and Social Sciences 1, 318-321.

Naz F, Haq IU, Asghar S, Shah AS, Rahman A. 2011. Studies on growth, yield and nutritional composition of different tomato cultivars in Battal valley of district Mansehra, Khyber Pakhtunkhwa, Pakistan. Sarhad J. Agric 27, 569-571.

Oliveira AM, Duarte V, Silveira JR, Moraes MG. 2003. Incidence of pectolytic erwinias associated with blackleg of potato in Rio Grande do Sul. Fitopatologia Brasileira 28, 49-53.

Ozturk M, Aksoy HM, Potrykus M, Lojkowska E. 2018. Genotypic and phenotypic variability of Pectobacterium strains causing blackleg and soft rot on potato in Turkey. European journal of plant pathology 152(1), 143-155.

Pakistan GO. 2015-2016. Ministry of national food security and research (nfs&r).

Pérombelon M, Kelman A. 1979. Population dynamics of Erwinia carotovora and pectolytic Clostridium spp. in relation to decay of potatoes [Erwinia carotovora carotovora, Erwinia carotovora atroseptica]. Phytopathology.

Perombelon MC, Kelman A. 1980. Ecology of the soft rot erwinias. Annual review of phytopathology 18, 361-387.

Pitman AR, Harrow SA, Visnovsky SB. 2010. Genetic characterisation of Pectobacterium wasabiae causing soft rot disease of potato in New Zealand. European Journal of Plant Pathology 126, 423-435.

Smeda N. 2009. District profile Mansehra. Small and Medium Enterprises Development Authority, Ministry of Industries and Production. Government of Pakistan.

Stahl W, Heinrich U, Wisema S, Eichler O, Sies H, Tronnier H. 2001. Dietary tomato paste protects against ultraviolet light–induced erythema in humans. The Journal of nutrition 131, 1449-1451.

Tamang JP, Tamang B, Schillinger U, Franzcm, Gores M, Holzapfel WH. 2005. Identification of predominant lactic acid bacteria isolated from traditionally fermented vegetable products of the Eastern Himalayas. International journal of food microbiology 105, 347-356.

Thomson S, Hildebrand D, Schroth M. 1981. Identification and Nutritional Differentiation of the Erwinia Sugar Beet Pathogen from Members of Erwinia carotovora and Erwinia chrysanthemi. Phytopathology 71, 1037-1042.

Van der Wolf J, De Haan E, Kastelein P, Krijger M, De Haas B, Velvis H, Van Der Zouwen P. 2017. Virulence of Pectobacterium carotovorum subsp. brasiliense on potato compared with that of other Pectobacterium and Dickeya species under climatic conditions prevailing in the Netherlands. Plant Pathology 66(4), 571-583.

Yusuf I, Oyaweye O, Yongabi K, Pemu A. 2004. Bacteriological Quality Assessment of Salad Vegetables sold in Bauchi Metropolis. Nigeria Journal of Microbiology 18, 316-320.