Molecular identification of four wild higher basidiomycetes collected in Mt. Mingan, Gabaldon, Nueva Ecija, Philippines

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Research Paper 01/12/2018
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Molecular identification of four wild higher basidiomycetes collected in Mt. Mingan, Gabaldon, Nueva Ecija, Philippines

Chenny Dhanryll M. Guzman, Marisol M. Baltazar, Aiza Jhane I. Sanchez, Mariel G. Linsangan, Rich Milton R. Dulay
J. Bio. Env. Sci.13( 6), 46-51, December 2018.
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Abstract

This paper reports the molecular identification of the four mushrooms collected in Mt. Mingan, Gabaldon, Nueva Ecija, Philippines. The DNA samples from the fresh fruiting bodies of mushrooms were extracted, isolated, purified, PCR amplified (using ITS-1F and ITS4 primers), and sequenced (using Capillary Electrophoresis Sequencing). The DNA sequences were trimmed and cleaned-up before sequence alignment using MEGA7 software and the phylogenetic affiliations of the ITS genes were determined by alignment to the NCBI non-redundant database using the BLASTn algorithm. The four mushrooms were identified as Stereum hirsutum (95%), Micropus xanthopus (99%), Pleurotus tuberregium (99%) and Trametes elegans (99%). To our knowledge, this is the first report on the collection and molecular identification of these wild mushrooms in Mt. Mingan.

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