J. Bio. Env. Sci.13( 6), 46-51, December 2018
This paper reports the molecular identification of the four mushrooms collected in Mt. Mingan, Gabaldon, Nueva Ecija, Philippines. The DNA samples from the fresh fruiting bodies of mushrooms were extracted, isolated, purified, PCR amplified (using ITS-1F and ITS4 primers), and sequenced (using Capillary Electrophoresis Sequencing). The DNA sequences were trimmed and cleaned-up before sequence alignment using MEGA7 software and the phylogenetic affiliations of the ITS genes were determined by alignment to the NCBI non-redundant database using the BLASTn algorithm. The four mushrooms were identified as Stereum hirsutum (95%), Micropus xanthopus (99%), Pleurotus tuberregium (99%) and Trametes elegans (99%). To our knowledge, this is the first report on the collection and molecular identification of these wild mushrooms in Mt. Mingan.
Aquino YKDC, Vega LDP, Medrano NRM, Dulay RMR. 2018. Mycochemicals, antioxidant and cytotoxic activities of Polyporus grammocephalus Berk (BIL7749). International Journal of Biology, Pharmacy and Allied Sciences 7(6), 966-975.
Amabye TG, Bezabh AM. 2015. Chemical composition and nutritional value of the most widely used mushrooms cultivated in Mekelie Tigray Ethiopia. American Journal of Applied Chemistry 3(5), 164-167.
Biadnes GCO, Tangonan NG. 2003. Assessment of the biodiversity of basidiomycetous fungi, insects and orchids in midmontane forest of Mt. Apo, Mindanao. PSSN Nature News 2, 59.
Bobek P, Nosalova P, Cerna S. 2001. Effect of pleuran (b-glucan from Pleurotus ostreatus) in diet or drinking ﬂuid on colitis in rats. Nahrung 45, 360–363.
Borchers A, Keen C, Me G. 2004. Mushrooms, tumors and immunity: an update. Exp. Biol. Med. 229(5), 393-406.
Daep NA, Cajuday LA. 2003. Mushroom Diversity at Mt. Malinao, Albay. PSSN Nature News 2, 57.
Dulay RMR, Maglasang CC. 2017. Species listing of naturally occurring mushrooms in agroecosystem of Barangay Bambanaba, Cuyapo, Nueva Ecija, Philippines. International Journal of Biology, Pharmacy and Allied Sciences 6(8), 1459-1472.
Dulay RMR, Miranda LA, Malasaga JS, Kalaw SP, Reyes RG, Hou CT. 2017. Antioxidant and antibacterial activities of acetonitrile and hexane extracts of Lentinus tigrinus and Pleurotus djamour. Biocatalysis and Agricultural Biotechnology 9, 141-144.
Gu YH, Sivam G. 2006. Cytotoxic effect of oyster mushroom Pleurotus ostreatus on human androgen-independent prostate cancer PC-3 cells. J Med Food. 9, 196–204.
Hu SH, Liang ZC, Chia YC, Lien JL, Chen KS, Lee MY, Wang JC. 2006. Antihyperlipidemic and antioxidant effects of extracts from Pleurotus citrinopileatus. J Agric Food Chemistry 54, 2103–2110.
Manter DK, Vivanco JM. 2007. Use of the ITS primers, ITS1F and ITS4, to characterize fungal abundance and diversity in mixed-template samples by qPCR and length heterogencity analysis. Journal of Microbial Methods.7-14.
Militante EP, Tadiosa E. 2005. Identification of important wood-decaying fungi associated with some Philippine dipterocarps at the Makiling forest. Ecosystems Research and Development Bureau. Dept. of Envi. and Natural Resouces.
Periasamy K. 2005. Novel antibacterial compounds obtained from some edible mushrooms. International Journal of Medicinal Mushrooms 7, 443– 444.
Rahi D, Malik D. 2016. Diversity of Mushrooms and Their Metabolites of Nutraceutical and Therapeutic Significance. Journal of Mycology 1-18.
Tang Z. 2004. Submerged cultivation of medicinal mushrooms for production of valuable bioactive metabolites. Advances in Biochemical Engineering / Biotechnology 87, 25-59.
Wang JC, Hu SH, Liang ZC, Yeh CJ. 2005. Optimization for the production of water-soluble polysaccharide from Pleurotus citrinopileatus in submerged culture and its antitumor effect. Appl Microbiol Biotechnol. 67, 759–766.
Yang JH, Mau JL, Lin HC. 2002. Antioxidant properties of several commercial mushrooms. Food Chemistry 77, 229–235.