Molecular marker based identification of leaf rust resistance gene Lr25 in selected wheat accessions

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Research Paper 01/12/2016
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Molecular marker based identification of leaf rust resistance gene Lr25 in selected wheat accessions

Sahib Gul Afridi, Habib Ahmad, Ikram Muhammad, Maryam Mobeen, Rimsha Zainab
Int. J. Biosci. 9(6), 266-270, December 2016.
Copyright Statement: Copyright 2016; The Author(s).
License: CC BY-NC 4.0

Abstract

Wheat (Triticum aestivum L.) rusts are among the most widespread and destructive diseases of wheat crop. Leaf rust, caused by Puccinia triticina Eriks is a common and widespread disease of wheat in several countries of the World including Pakistan. Molecular survey was conducted to screen altogether 19 Pakistani wheat germplasm/accessions obtained from National Agriculture Research Council (NARC) Islamabad, Pakistan. For the presence of leaf rust resistance gene Lr25, specific SSR primers linked to Lr25 were utilized. The research studies revealed that out of the 19 accessions grown in the botanical garden at Abdul Wali Khan University Mardan during the month of March and April 2013, 4 accessions were observed having Lr25 gene, which showed high resistance. Two accessions revealed moderate resistance while 13 accessions did not show the presence of Lr25 gene and were regarded as susceptible to leaf rust disease. The identification of Lr25 in Pakistani wheat germplasm will help in accelerating the breeding program in future, including pyramiding of different wheat resistant genes in wheat varieties.

Anonymous. 2014. Pakistan Economic Survey, pp.28. Ministry of Food, Agriculture and Livestock Federal Bureau of Statistics Islamabad, Pakistan.

Anonymous. 2005. Report of screening of advance lines against yellow and leaf rusts under national climatic conditions of Barani Agriculture research station Kohat. Pakistan Journal of Agronomy 2, 8-12.

Babar M, Mashhadi AF, Mehvish A, Zahra AN, Hasnain A, Hussain N, Ali M, Khaliq I, Aziz A. 2010. Identification of rust resistance genes Lr10 and Sr9a in Pakistani wheat germplasm using PCR based molecular markers. African Journal of Biotechnology 9(8), 1144 -1150. http://dx.doi.org/10.5897/AJB10.1551

Chen XM. 2013. High-temperature adult plant resistance, key for sustainable control of stripe rust. American Journal of Plant Sciences 4, 608-627.  http://dx.doi.org/10.4236/ajps.2013.43080

Dixon J, Braun HJ, Crouch JH. 2009. Overview: transitioning wheat research to serve the future needs of the developing world. In: J. Dixon, H.-J. Braun, P. Kosina and J. Crouch (eds.), Wheat Facts and Futures 2009. Mexico, D.F: CIMMYT.

Fahmi AI, Nazim M, Khalifa SZ, El-Orabey 2005. Genetics of adult plant resistance to leaf rust in Egyptian wheat. Egyptian Journal of Phytopathology 33, 1-10.

FAO. 2014. Food and Agriculture Organization. Available online at. www.fao.org.

Hovmøller MS, Amor H, Yahyaoui EA, Annemarie FJ. 2008. Rapid global spread of two aggressive strains of a wheat rust fungus. Molecular Ecolology 17, 3818−3826. http://dx.doi.org/10.1111/j.1365-294X.2008.03886.x

Huerta-Espino J. Singh R, German S, McCallum B, Park R, Chen W, Bhardwaj S, Goyeau H. 2011. Global status of wheat leaf rust caused by Puccinia triticina. Euphytica 179, 143-160. http://dx.doi.org/10.1007/s10681-011-0361-x

Jellis GJ. 2009. Crop Plant Resistance to Biotic and Abiotic Factors: Combating the Pressures on Production Systems in a Changing World. Crop Plant Resistance to Biotic and Abiotic Factors: Current Potential and Future Demands. Proceedings of the 3rd International Symposium on Plant Protection and Plant Health in Europe, pp:15−20.

Khan MA. 1987. Wheat variety development and longevity of rust resistance. Government of Punjab Agriculture Department, Lahore. 196-197.

Kolmer JA. 1996. Genetics of resistance to wheat leaf rust. Annual Review of Phytopathology 34, 435–55. http://dx.doi.org/10.1146/annurev.phyto.34.1.435

Malik TA, Iqbal A, Chowdhry MA, Kashif M, Sajid-Ur-Rahman. 2007. DNA marker for leaf rust disease in wheat. Pakistan Journal Botany 39, 239-243.

McIntosh RA. 1988. The role of specific genes in breeding for durable stem rust resistance in wheat and triticale. In Simmonds NW and Rajaram S (ed.). Breeding strategies for resistance to the rusts of wheat. CIMMYT, El Batan, Mexico. 1-9 P.

McIntosh RA, Dubcovsky J, Rogers WJ, Morris C, Appels V, Xia XC. 2012. Catalogue of gene symbols for wheat: 2012 supplement. Annals of Wheat Newsletter 57, 303-321.

Ogbonnaya F, Mujeeb-Kazi A, Kazi AG, Lagudah EL, Xu SS, Bonnett D. 2013. Synthetic hexaploid in wheat improvement. In: J. Janick (ed.), Plant Breeding Reviews; 35-122 P. John Wiley & Sons Inc.

Park RF, Wellings CR, Bariana HS. 2007. Preface to global landscapes in cereal rust control. Australian Journal of Agriculture Research 58, 469. http://dx.doi.org/10.1071/ARv58n6PR

Park RF, Mohler V, Nazari K, Singh D. 2014.Characterization and mapping of gene Lr73 conferring seedling resistance to Puccinia triticina in common wheat. Theoretical Applied Genetics 127, 2041-2049. http://dx.doi.org/10.1007/s00122-014-2359-y

Pathan AK, Park RF. 2006. Evaluation of seedling and adult plant resistance to leaf rust in European wheat cultivars. Euphytica 149, 327–342. http://dx.doi.org/10.1007/s10681-005-9081-4

Rizvi SSA, Hussain M, Aslam M. 1984. Pathogenicity association in puccinia recondite f. sp. tritici in Pakistan. Cereal rust communication 12, 151-157.

Roelfs AP, Singh RP, Saari EE. 1992. Rust diseases of wheat: concepts and methods of disease management. CIMMYT, Mexico, D.F.

Rosewarne GM, Herrera-Foessel SA, Singh RP, Huerto-Espino J, Lan CX, He ZH. 2013. Quantitative trait loci of stripe rust resistance in wheat. Theoretical Applied Genetics 126, 2427-2449. http://dx.doi.org/10.1007/s00122-013-2159-9

Sleper DA, Poehlman JM. 2006. Breeding field crops, 5th Ed. Blackwell Publishing Professional. 2121 State Avenue, Ames, Iowa.

Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18, 6531–6535.  http://dx.doi.org/10.1093/nar/18.22.6531

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