Molecular morphogical and physiological study in Tunisian wild wheat relative Aegilops geniculata Roth and wheat (Triticum durum Desf.) under salt stress

Paper Details

Research Paper 01/12/2014
Views (264) Download (12)
current_issue_feature_image
publication_file

Molecular morphogical and physiological study in Tunisian wild wheat relative Aegilops geniculata Roth and wheat (Triticum durum Desf.) under salt stress

Mahjoub Asma, Mguis Khaled, Ben Brahim Nadia
Int. J. Agron. Agri. Res.5( 6), 20-35, December 2014.
Certificate: IJAAR 2014 [Generate Certificate]

Abstract

In this investigation, an attempt was made to assess the genetic diversity among thirteen Aegilops geniculata Roth populations and three durum wheat varieties originated from different bioclimatic areas using 19 RAPD markers. For morpho-physiological traits we have selected three populations of Aegilops geniculata and one variety of durum wheat from each bioclimatic area. This study has shown a high degree of variation of these characters mainly related to geographical origin. It was observed also that the Sbeitla population was less affected by the imposed salt stress than all the others while Ain zana was the most affected one. The CO2 assimilation rate, stomatal conductance, and intercellular CO2 for the three Ae. geniculata populations and wheat variety significantly decreased with increasing salt. Among the factors inhibiting photosynthetic activity, those of a stomatal nature had a greater effect. The molecular study indicated an important inter-specific polymorphism between Aegilops and durum wheat. Ae. geniculata populations revealed a high level of polymorphism (71.27%) than wheat cultivars (39.76%). Two main groups were represented by cluster analyses. The first group is formed by Ae. geniculata populations and the second is constituted only by durum wheat cultivars.

VIEWS 16

Abassi AM. 2009. Tolérance de divers clone de Peuplier à la salinité : Aspects morphologiques, Ecophysiologiques, Métaboliques, Anatomiques et Ultra-Structuraux. Thesis University Tunisia

Allakhverdiev SI, Sakamoto A, Nishiyama Y, Inaba M, Murata N. 2000. Ionic and osmotic effects of NaCl-induced inactivation of photosystems I and II in Synechococcus sp. Plant Physiol 123,1047-1056.

Anand A, Rao CS, Eganathan P, Kumar NA and Swaminathan MS. 2004. Saving an endangered Taxon: Syzygium travancoricum Gamble: A case study focussing on its genetic diversity, and reintroduction. Physiol. Mol. Bio. Plants 10, 233-242

Ashraf M, Foolad MR. 2007. Improving plant abiotic-stress resistance by exogenous application of osmoprotectants glycinebetaine and proline. Environ. Exp. Bot 59, 206-216.

Baghizadeh A, Khosravi S. 2011. Genetic diversity assessment of Aegilops germplasm by RAPD molecular markers. Agric. Biol. J. N. Am 2(2), 197-202

Béjaoui Z. 2006. Tolérance de divers clones de peuplier à l’hydromorphie: aspects morphologiques, écophysiologiques et métaboliques. Thesis University Tunisia

Belkhodja R, Morales F, Abadia A, Medrano H, Abadia J. 1999. Effects of salinity on chlorophyll fluorescence and photosynthesis of barley (Hordeum vulgare L.) grown under a triple-linesource sprinkler system in the field. Photosynthetica 36,375–387

Boeger JM, Chen RS, MCdonald BA. 1993. Gene flow between geographic populations of Mycosphuerellu gruminicolu (Anamorph Septoriu tritici) detected with restriction fragment length polymorphism markers. Phytoputhology 83, 1148-1154.

Chalmers K, Campbell AW, Krestschmer J, Karakousis A, Henschke PH, Pierens S, Harker N, Pallotta M, Cornish GB, Sharillou MR, Rampling LR, Mclauchlan A, Daggard G, Sharp PJ, Holton TA, Sutherland MW, Appels R, and Langridge P. 2001.Construction of three linkage maps in bread wheat, Triticum aestivum L. Austr. J. Agric. Research 52, 1089-1119.

Colmer TD, Flowers TJ, Munns R. 2006. Use of wild relatives to improve salt tolerance in wheat. J Exp Bot 57,1059–1078

Cramer GR, Quarrie SA. 2002. Abscissic acid is correlated with the leaf growth inhibition of four genotypes of maize differing in their response to salinity. Funct Plant Biol 29,111–115

Cronic G, Massacci A. 1996. Leaf photosynthesis under drought stress. In: Baker NR ed. Photosynthesis and the Environment. The Netherlands: Kluwer Academic Publishers, Dordrecht- Boston-London, p.347–366

Cuénod  A,  Pottier-Alapetite  G,  et  Labbe  A. 1954. La flore analytique et synoptique de la Tunisie. Cryptogames vasculaires, gymnospermes et monocotylédones. Office de l’Exp. et de la Vulg. agricole de Tunisie – SEFAN Tunis.

Dalton FN, Maggio A, Piccinni G. 2001. Assessing the effect of solar radiation on plant salt tolerance as defined by the static and dynamic indices. Plant Soil 229, 189-195.

Dionisio-Ses ML, Tobita S. 2000. Effects of salinity on sodium content and photosynthetic responses of rice seedlings differing in salt tolerance. J Plant Physiol 157, 54–58

Do Amaral Júnior AT, de Oliveira ÉC, Gonçalves LSA., Scapim CA, Candido LS, da Conceição Silva TR, Vittorazzi C, and da Cunha KS. 2011. Assessment of genetic diversity among maize accessions using inter simple sequence repeats (ISSR) markers, African Journal of Biotechnology 10(69), 15462-15469

El Aouni MH. 1980. Processus déterminant la production du pin d’Alep (Pinus halepensis Mill): photosynthèse, croissance et répartition des assimilats. Thesis University, Paris VII.

Eujayl I, Sorrells ME, Baum M, Wolters P and Powell W. 2002. Isolation of EST-derived microsatellite markers for genotyping the A and B genomes of wheat. Theor. Appl. Genet 104, 399-407

Farooq S, Shah TM, Asghar M. 1996. Intergeneric hybridization for wheat improvement: V. Production of and metaphase 1 chromosome analysis in F1 hybrids of wheat (Triticum aestivum) with Aegilops ovata L. Cereal Res Commun 24(2), 155–161

Flowers TJ, Yeo AR. 1981. Variability in the resistance of sodium chloride salinity within rice (Oryza sativa L.) varieties. New Phytol 88, 363–373

Froster BF, Gorham J, Miller TE. 1987. Salt tolerance of an amphiploid between Triticum aestivum and Agropyron junceum. Plant Breed 98, 1–8

Ingvarsson PK. 2001. Restoration of genetic variation lost–the genetic rescue hypothesis. Trends Ecol. Evol 16, 62-63.

Kadri K, Abdellawi R, Cheikh-Mhamed H, Ben-Naceur M and Trifi M. 2010. Analysis of Genetic diversity and relationships in local Tunisian barley by RAPD and SSR analysis. African Journal of Biotechnology 9(44), 7429-7436

Kellogg EAR and Mason-Gamer ARJ. 1996. When genes tell different stories: the diploid genera of Triticeae (Gramineae). Systematic Botany 2, 11-17.

Kao WY, Tsai TT, Tsai HC, Shih CN. 2006. Response of three Glycine species to salt stress. Environ Exp Bot 56,120–125

Kim HJ, Fonseca JM, Choi JH, Kubota C, Kwon DY. 2008. Salt in irrigation water affects the nutritional and visual properties of Romaine lettuce (Lactuca sativa L.). J. Agr Food Chem 56(10), 3772–3776.

Li HJ, Conner RL, Chen O, Li HY, Laroche A, Graf R J and Kuzyk AD. 2004. The transfer and characterization of resistance to common root rot from Thinopyrum ponticum to wheat. Genome 47, 215-223.

Maas EV, Poss JA, Hoffman GJ. 1986. Salinity sensitivity on sorghum at three growth stages. Irrig Sci 7, 1–11

Monneveux P, Zaharieva M, Rekika D. 2000. The utilization of Triticum and Aegilops species for the improvement of durum wheat. Amélioration génétique des Plantes INRA 340, 71–81

Martin-Sanchez JA, Gomez-Colmenarejo M, Del Moral J, Sin E, Montes MJ, Gonzalez-Belinchon C, Lopez-Brana I and Delibes A . 2003. A new Hessian fly resistance gene (H30) transferred from the wild grass Aegilops triuncialis to hexaploid wheat. Theor. Appl. Genet 106, 1248-1255.

Mguis K. 2010. Comportement morphologique, ecophysiologique et nutritionnel de trois accessions d’Aegilops geniculata (Roth.) et d’un cultivar du blé dur soumis à la contrainte saline. Thesis University Tunisia

Munns R, Rawson HM. 1999. Effect of salinity on salt accumulation and reproductive development in the apical meristem of wheat and barley. Aust J Plant Physiol 26,459–464

Murray MG and Thompson WF. 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acid Research 8, 4321-4325.

Neel MC and Ellstrand NC. 2003. Conservation of genetic diversity in the endangered plant Eriogonum ovalifolium var. vineum. Conservation Genetics 4, 337-352

Nei M. 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89, 583-590

Netondo GW, Onyango JC, Beck E. 2004a. Sorghum and salinity: I. Response of growth, water relations, and ion accumulation to NaCl salinity. Crop Sci 44,797–805

Netondo  GW,  Onyango  JC,  Beck  E.  2004b. Sorghum and salinity: gas exchange and chlorophyll fluorescence of sorghum under salt stress. Crop Sci 44, 806–811

Nevo E, Korol AB, Beiles A and Fahima T. 2002. Evolution of Wild Emmer and Wheat Improvement. Population Genetics, Genetic Resources and Genome Organization of Wheat’s Progenitor, Triticum dicoccoides. Springer, Heidelberg. plant Eriogonum ovalifolium var. vineum. Conservation Genetics 4, p.337-352.

Nieto-Lopez RM, Soler C, Garcia P. 2003. Genetic diversity in wild Spanish populations of Thinopyrum junceum and Thinopyrum junceiforme using endosperm proteins and PCR-based markers. Hereditas 139, 18–27

Perrino P, Laghetti G, Cifarelli S, Volpe N, Spagnoletti-Zeuli PL. 1993. Wild wheats in southern Italy. In: Damania AB ed. Biodiversity and wheat improvement. John Wiley and Sons, Chichester, UK, p.361–368

Rabey HA, Badr A, Schafer PR, Salamini WM. 2002. Speciation and species separation in Hordeum L. (Poaceae) resolved by discontinuous markers. Plant Biol 4, 1-9

Richards CM. 2000. Inbreeding depression and genetic rescue in a plant metapopulation. Am. Nat 155, 383-394.

Sambrook J, Fritish EF, Maniatis T. 1989. Molecular cloning a laboratory manual 2ed. Clod spring Harbor laboratory, New York.

Sharma PK, Hall DO. 1991. Interaction of salt stress and photoinhibition on photosynthesis in barley and sorghum. J Plant Physiol 38, 614–619

Siahsar BA, Allahdoo M and Shahsevand Hassani H. 2011. Genetic variation among and within tritipyrum (Thinopyrum bessarabicum × Triticum durum) lines using PCR-based molecular markers. Cien. Inv. Agr 38(1), 127-135.

Silva TDJA, Yonekura L, Kaganda J, Mookdasanit J, Nhut DT and Afach G. 2005a. Important secondary metabolites and essential oils of spices within the Anthemidae (Asteraceae). J. Herbs Spices Med. Plants 11, 1-46.

Silva TDJA., Nhut DT, Giang DTT and Rashid SZ. 2005b. Molecular markers assisted phylogeny and breeding in ornamental, physiological model, medicinal and aromatic plants. In: Genetic Resources, Biotechnolgy, Eds. D. Thangadurai, T. Pullaiah, M.A. Pinheiro de Carvalho. vol.111. Regency Publications. New Delhi, India, p.309-326.

Terashima I, Ono K. 2002. Effects of HgCl2 on CO2 dependence of leaf photosynthesis: evidence indicating involvement of aquaporins in CO2 diffusion across the plasma membrane. Plant Cell Physiol 43, 70–78

Tezara W, Martinez D, Rengifo E, Herrera A. 2003. Photosynthetic response of the tropical spiny shrub Lycium nodosum (Solonacea) to drought, soil salinity and saline spray. Ann Bot 92, 527–765

van Slageren MW. 1994. Wild Wheats: a monograph of Aegilops L. and Amblyopyrum (Jaub. and Spach) Eig (Poaceae). Wageningen Agricultural University Papers 94–7, Wageningen, the Netherlands.

Vinocur B, Altman A. 2005. Recent advances in engineering plant tolerance to abiotic stress: achievements and limitations. Curr. Opin. Biotechnol 16,123-132.

Warran CR, Livingston NJ, Turpin DH. 2004. Water stress decreases the transfer conductance of Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) seedlings. Tree Physiol 24, 971–979

Wright S. 1931. Evolution in Mendelian populations. Genetics 16, 97-159.

Wu CJ, Cheng ZQ, Huang XQ, and Yin SH. 2004. Genetic diversity among and within population of Oryza Granulate from Yunnan of China revealed by RAPD and ISSR markers: implications for conservation of the endangered species. Plant Sci 167, 35-42.

Yeh FC, Yang R and Boyle T. 1999. POPGENE: Microsoft Windows-based freeware for population genetic analysis. Release 1.31. University of Alberta. Canada. http://www. ualberta.ca/~fyeh/index.htm.

Zaharieva M, Prosperi JM, Monneveux P. 2004. Ecological distribution and species diversity of Aegilops L. genus in Bulgaria. Biodivers. Conserv 13, 2319-2337.

Zaharieva M, Gaulin E, Havaux M, Acevedo E and Monneveux P. 2001. Drought and heat responses in the wild wheat relative Aegilops geniculata Roth: potential interest for wheat improvement. Crop Sci 41,1321-1329