Paper Details

Molecular variation of improved durum wheat genotypes based on inter-simple sequence repeats fingerprinting

Samar Shirnasabian, Alireza Etminan, Reza Mohammadi, Lia Shooshtari

DOI: https://dx.doi.org/10.12692/ijb/5.12.222-228

Int. J. Biosci. 5(12), 222-228. December, 2014. (PDF)

Abstract:

Durum wheat (Triticum turgidum L. var. durum) is an important crop with high protein content and superior cooking quality which used for Pasta production. In present  study, the molecular diversity of eighteen durum wheat breeding lines along with one durum wheat check cultivar were evaluated based on inter simple sequence markers. Eight used primers amplified a total of 93 bands, among which 77 bands (about 83%) were polymorphic. A total of 77 polymorphic fragments were scored with average 9.6 polymorphic bands per primer. The polymorphism information content value ranged between 0.27 and 0.36 for ISSR primers. Cluster analysis using UPGMA method and Dice similarity coefficient grouped the 19 genotypes into four separated clusters. The Dice similarity coefficients revealed maximum similarity (0.91) between 20A-URDYT-10 and  20A-URDYT-11 genotypes. PCA based on molecular data distinguished genotypes similar to the cluster analysis results. The results revealed that ISSR technique is a simple, informative and suitable approach for assessment of molecular diversity in durum wheat.