Mollifying actions of garlic (Allium sativum) against Chloropyrifos induced toxicity by using rabbit (Oryctolagus cuniculus) as a model subject
Paper Details
Mollifying actions of garlic (Allium sativum) against Chloropyrifos induced toxicity by using rabbit (Oryctolagus cuniculus) as a model subject
Abstract
Potency of garlic is being acknowledged for years and is frequently used as a remedy for different diseases. In developing countries, the widespread use of pesticides in daily life has caused severe environmental pollution and consequently health hazards, with cases of severe acute and chronic poisoning in organisms. Recently, a growing interest has been shown into utilizing the biological activities of different medicinal herbs against diseases, due to their natural origin, lesser side effects and cost effectiveness. The current study was designed to evaluate the therapeutic role of garlic against the Chloropyrifos (CPF) induced toxicity in male rabbits (Oryctolagus cuniculus). For this purpose, male healthy rabbits (N=24) were procured from the local market and were divided into eight groups on the basis of acute and chronic toxicity evaluations. Group “A” and A1 were left untreated and were considered as the control group for acute and chronic toxicity trials respectively. Group “B” and B1 were the Garlic fed group while group “C” and C1 were the Chloropyrifos (CPF) treated group, while group “D” and D1 were garlic co Chloropyrifos (CPF) administered group. Rabbits were orally administered with doses of garlic and CPF for a period of 14 and 60 days on daily basis for evaluating acute and chronic toxicity respectively. At the end of the trials, blood samples were collected in tubes for investigation of the hematological indices. Hematological analyzer (Sysmex KX-21 N, USA made) was used for analysis. The tested parameters showed a variability in values in comparison to the untreated group. In the acute toxicity experiment, a significant percent decrease in the level of Hb (9.83%), RBCs (16.54%), WBCs (20.64%), HCT(12.63%), Plts(5.5%) were observed in pesticide treated group while in garlic fed group the level of Hb (13.44%), RBCs (15.8%) ,WBCs (29.8%), HCT(21.5%), Plts (15%) showed % increase as compare to CPF and Control groups. In CPF co- Garlic administered group, garlic has attenuated the toxic effects of CPF. In conclusion CPF is found to be hematotoxic in nature both in acute and chronic doses and Garlic was found to be protective against its toxicity hence reflecting its importance. Therefore it could be advised as an effective dietary supplement in the developing countries where the use of pesticide is high.
Abou-Donia, Mohamed B. 2003. Organophosphorus ester-induced chronic neurotoxicity.” Archives of Environmental Health: An International Journal 58(8), 484-497.
Ahlam FAS, 2009. Some toxicological studies of some pesticides on male albino rats. PH. D., V.Sc. Foresinic Medicine and Toxicology, Benha University Egypt.
Ahmad L, Khan A, Khan MZ, Hussain I. 2009. Cypermethrin induced anemia in male Rabbits. Pakistan Veterinary Journal 29(4), 191-195.
Al-Jowari SAK. 2014. Effect of Garlic Powder (Allium sativum) on Blood Constituents in Male Rabbits. Al-Nahrain Journal of Science 17(3), 132-137. https://anjs.edu.iq/index.php/anjs/article/view/416/360
Ambali SF, Akanbi D, Igbokwe N, Shittu M, Kawu M, Ayo J. 2007. Evaluation of subchronic Chlorpyrifos poisoning on hematological and serum biochemical changes in mice and protective effect of vitamin C. Journal Of Toxicological Sciences 32(2), 111-120. https://doi.org/10.2131/jts.32.111
Ambali SF, Ayo JO, Ojo SA, Esievo KAN. 2010. Co-administration of vitamins C and E ameliorates chronic chlorpyrifos-induced erythrocyte osmotic fragility in Wistar rats. Australian Journal of Basic and Applied Sciences 4(6), 1051-1021. http://www.insipub.com/ajbas/2010/1015-1021.pdf
Ambali SF, Ayo JO, Esievo KA, Ojo SA. 2011. Hem toxicity induced by chronic chlorpyrifosexposure in wistar rats: mitigating effect of Vitamin C. Veterniery Medicine International 2011. 945439. http://dx.doi.org/10.4061/2011/945439
Ambali SF, ANGANI M, ADOLE AO, KAWU MU, SHITTU M. 2011. Protective effect of vitamin C on biochemical alterations induced by subchronic co-administration of chlorpyrifos and lead in Wistar rats. Journal of Environmental and Analytical. Toxicology 1, 108-10. http://dx.doi.org/10.4172/2161-0525.1000108
Araujo CF, Lacerda MVG, Abdalla DSP, Lima ES. 2008. The role of platelets and plasma markers of antioxidant status and oxidative stress in thrombocytopenia among patients with vivax malaria. Memorias do Instituto Oswaldo Cruz 103(6), 517–521. http://dx.doi.org/10.1590/S007402762008000600001
Auta J. 2001. Toxicity of dimethoate to juveniles of Oreochromis niloticus and Clarias gariepinus. Ph.D.thesis. Biological sciences department,Ahmedo Bello University Zaria, Nigeria.
Banaee M, Mirvagefei AR, Rafei GR, Majazi Amiri B. 2008. Effect of sub-lethal diazinon concentrations on blood plasma biochemistry. International Journal of Environmental Research 2(2), 189-198.
Banaee M, Sureda A, Mirvaghefi AR, Ahmadi K. 2011. Effects of diazinon on biochemical parameters L,of blood in rainbow trout (Oncorhynchus mykiss). Pesticide biochemistry and physiology 99(1), 1-6. https://doi.org/10.1016/j.pestbp.2010.09.001
Banerjee SK, Maulik SK. 2002. Effect of garlic on cardiovascular disorders: a review. Nutrition journal 1(1), 4. https://doi.org/10.1186/1475-2891-1-4
Blakley BR, Yole MJ, Brousseau P, Boermans H, Fournier M. 1999. Effect of chlorpyrifos on immune function in rats. Veterinary and Human Toxicology 41, 140-144.
Bordia A, Arora SK, Kothari LK, Jain KC, Rathore BS, Rathore AS, Bhu N. 1975. The protective action of essential oils of onion and garlic in cholesterol-fed rabbits. Atherosclerosis 22(1), 103 109. https://doi.org/10.1016/0021-9150(75)90071-4
Cox C. 1994. Chlorpyrifos, Part 1 (Toxicology). Journal of Pesticide Reforms 14(4), 15- 20.
Chan KC, Yin MC, Chao W J, 2006. Effect of dially trisulfide rich garlic oil on blood coagulation and plasma activity of anticoagulation factors in rats. Food Chemical Toxicology 45(3), 502–507. https://doi.org/10.1016/j.fct.2006.10.005
Dam K, Garcia SJ, Seidler FL, Slotkin TA .1999. Neonatal chlorpyrifos exposure alters synaptic development and neuronal activity in cholinergic and catecholaminergic pathways. Developmental Brain Research 116, 9- 12. https://doi.org/10.1016/S0165-3806(99)00067-X
Dargel R. 1992. Lipid peroxidation—a common pathogenetic mechanism. Experimental and Toxicologic Pathology 44(4), 169–181. https://doi.org/10.1016/S0940-2993
Das KK, Gupta AD, Dhundasi SA, Patil AM, Das SN, Ambekar JG. 2007. Protective Role of L-ascorbic acid on Antioxidant Defense System in Erythrocytes of Albino Rats Exposed to Nickel Sulfate. Biometals 20, 177–184. https://doi.org/10.1007/s10534-006-9025-z
Flora S J, Mehta A, Gupta R. 2009. Prevention of arsenic-induced hepatic apoptosis by concomitant administration of garlic extracts in mice. Chemico-biological interactions 177(3), 227-233. https://doi.org/10.1016/j.cbi.2008.08.017
Gamble SC, Goldfarb PS, Porte C, Livingstone DR. 1995. Glutathione peroxidase and other antioxidant enzyme function in marine invertebrates (Mytilus edulis, Pecten Maximus, Carcinus maenas and Asterias rubens). Marine Environmental Research 39, 191–195. https://doi.org/10.1016/0141-1136(94)00031-J
Gill TS, Pant JC. 1985. Mercury-induced blood anomalies in the freshwater teleost. Water,Air and Soil Pollution 24(2), 165-171, 0049. https://doi.org/6979/85/0242-0165501.05.
Gultekin F, Ozturk M, Akdogan M. 2000. The effect of organophosphate insecticide, chlorpyrifos-ethyl on lipid peroxidation and antioxidant enzymes (in-vivo). Archives of Toxicology 74, 533-538. https://doi.org/10/1007/s00204000167
Gultekin F, Patat S, Akca H, Akdogan M, Altuntas I.2006. Melatonin can suppress the cytotoxic effects of chlorpyrifos on human hepG2 cell lines. Human Experimental Toxicology 25(2), 47-55. https://doi.org/10.1191%2F0960327106ht584oa
Guyton A, Hall J. 2006.text book of medical physiology,11th.
Goel A, Dani V, Dhawan Dk. 2005. Protective effects of zinc on lipid peroxidation, antioxidant enzymes and hepatic histoarchitecture in CPF-induced toxicity. Chemico-Biological Interactions, 156, 131-140. https://doi.org/10.1016/j.cbi.2005.08.004
Goel A, Danni V, Dhawan DK. 2006(b). Role of zinc in mitigating the toxic effects of chlorpyrifos on hematological alterations and electron microscopic observations in rat blood. BioMetals 19, 483-492. https://doi.org/10.1007/s10534-005-5148-x
Iqbal F. 2012. Toxic effects of carbofuran and permethrin on the biochemical and hematological parameters in male rabbit’s .M.S Thesis, Islamia College University, and Peshawar Pakistan p32.
Jeorg HG, Lee YW. 1998. Protective effect of daily sulfide on N-nitrosodimethylamine-induced immunosuppression in mice. Cancer Letter 11, 73-9. https://doi.org/10.1016/S0304-3835(98)00246-8
Kumar N, Prabhu PAJ, Pal AK, Remya S, Aklakur M, Rana RS, Gupta S, Raman RP, Jadhao SB. 2011. Anti-oxidative and immuno-hematological status of Tilapia (Oreochromis mossambicus) during acute toxicity test of endosulfan. Pesticide Biochemistry and Physiology 99, 45 -52. https://doi.org/10.1016/j.pestbp.2010.10.003
Latimer K S, Prasse K W. 2003. Leukocytes. In Duncan and Prasse’s Veterinary Laboratory Medicine: Clinical Pathology (K. S. Latimer, E. A. Mahaffey, and K.W. Prasse, eds.), 46—79. Wiley-Blackwell, Ames, IA.
Miller H, Rigelhof F, Prakash A, Kanter M. 2000. Antioxidant content of whole grain breakfast cereals, Fruits And Vegetables. Journal of American College of Nutrition. 19, 1-8. https://doi.org/10.1080/07315724.2000.10718966
Modesto KA, Martinez CBR. 2010. Effects of Roundup Transorb on fish: hematology, antioxidant defenses, and acetylcholinesterase activity. Chemosphere 81, 781–787. https://doi.org/10.1016/j.chemosphere.2010.07.005
Muttappa K, Reddy HRV, Padmanabha A, Prabhudeva K, Rajanna K, Chethan. 2015. Combined effect of cadmium and chlorpyrifos on hematological Changes in tilapia (Oreochromismossambicus) n2. International journal of recent scientific research 6(3), 2981-2985.
Murray RK, Granner DK, Mayes PA. Rodwell VW. 1999. Harper’s Biochemistry, 26th edition, PrenticeHall, USA, p. 626-27.
Okolie NP, Aligbe JU, Osakue EE. 2004. Phostoxin-induced biochemical and pathomorphological changes in rabbits. Indian Journal of Experimental Biology 42(11), 1096-9.
Olaniyan OT, Meraiyebu AB, Arogbonlo A, Dare JB, Shekins O, Shafe MO. 2013. Effects of Aqueous Extract of Garlic (Allium sativum) on Blood Parameters in Adult Wistar Rats (Rattus novergicus) International Journal of Pharmceutical Scientific Inventions 2, 42-44.
Oluwole FS. 2001. Effects of garlic on some hematological and biochemical parameters. African Journal of Biomedical Research 4, 139-141. http://dx.doi.org/10.4314/ajbr.v4i3.53900
Parekh J, Chanda S. 2007. Antibacterial and phytochemical studies on twelve species of Indian medicinal plants. African Journal of Biomedical Research. 10(2), 175 -181.
Parsad R, Rose MK, Virmani M, Garg GL, Puri JP. 2009. Effect of garlic (Allium sativum) supplementation on Haematological parameters in chicken (Gallus domesticus). Indian journal of Animal Research 43(3), 157-162.
Pedrazza-Chaverri J, Tapia E, Medina-Campos O, De Los Angeles Granados M, Franco M. 2006. Garlic prevents hypertension induced by chronic inhibition of nitric oxide synthesis. Life Sciences 62, 71-77. https://doi.org/10.1016/S0024-3205(97)01155-7
Ramesh M, Saravanan. 2008. Hematological and biochemical responses in a freshwater fish Cyprinus carpio exposed to Chloropyrifos. International Journal of Integrative Biology.3.(1), 80-83.
Riaz M, Yousafzai AM. 2017. Toxic effects of insecticides Malathion and cypermethrin on hematological parameters in blood of male rabbits (Oryctolagus cuniculus). Journal of Entomology and Zoological Studies 5(3), 154-158.
Salih E. 2010. Toxic effect of dimethoate and diazinon on the biochemical and hematological parameters in male rabbits. Jordan Journal of Biological Sciences 147(613), 1-12.
Savithri Y, Ravi Sekhar P, Jacob Doss P. 2010. Changes in hematological profiles of albino rats under chlorpyrifos toxicity. International Journal of Pharmacological and Biological Sciences 1(3), pBS18. http://www.ijpbs.net/issue-3/19.pdf
Shaheen M, Yousafzai AM. 2017. Investigation of acute toxicity of Malathion and Cypermethrin on Clinico-hematological parameters in female New Zealand White Rabbits (Oryctolagus cuniculus). Journal of Entomology and Zoological Studies 5(3), 1045-1050.
Svoboda M, Luskova V, Dratichova J, Zlabek V. 2001. The effect of Diazinon on hematological indices of common carp (Cyprinus carpio L).Journal of ACTA Veternaria Brno 70, 465-475
Toghyani M, Gheisari A, Ghalamkari G, Eghbalsaied S. 2011. Evaluation of cinnamon and garlic as antibiotic growth promoter substitutions on performance, immune responses, serum biochemical and hematological parameters in broiler chicks. Livestock Sciences 138, 167–173. https://doi.org/10.1016/j.livsci.2010.12.018
Slotkin TA, Levin ED, Seidler FJ. 2006. Comparative developmental neurotoxicity of organophosphate insecticides: Effects on brain development are separable from systemic toxicity. Environmental HealthPerspective 114, 746-751. https://doi.org/10.1289/ehp.8828
Thomson M, Al-Amin ZM, Al-Qattan KK, Shaban LH, Ali M. 2007. Anti-diabetic and hypolipidaemic properties of garlic (Allium sativum) in streptozotocin-induced diabetic rats. International Journal of Diabetes & Metabolism 15, 108-115.
Thrasher JD, Madison R, Broughton A. 1993. Immunologic abnormalities in humans exposed to chlorpyrifos: preliminary observations. Archives of Environmental Health 48(2), 89-93. https://doi.org/10.1080/00039896.1993.9938400
Thrasher JD, Heuser G, Broughton A. 2002. Immunological abnormalities in humans chronically exposed to chlorpyrifos. Archives of Environmental Health 57, 181 187. https://doi.org/10.1080/00039890209602934
Tomlin CDS. 2006. The Pesticide Manual, A World Compendium, British Crop Protection Council: Alton, Hampshire, UK., 14th ed: p 186-187.
Uchendu C. 2011. “Evaluation of the effects of ascorbic acid and acetyl-L-carnitine on subacute chlorpyrifos poisoning in Wistar rats”, M.Sc. thesis, Ahmadu Bello University, Zaria, 176 p.
Ural MS. 2013.Chlorpyrifos-induced changes in oxidant/antioxidant status and hematological parameters of Cyprinus carpio: Ameliorative effect of lycopene. Chemosphere 90, 2059–2064. https://doi.org/10.1016/j.chemosphere.2012.12.006
Vale JA.1998. Toxikinetic and toxicodynamic aspects of organophosphorus aspects of insecticide poisioning. Toxicology letters 102, 649-652. https://doi.org/10.1016/S0378-4274(98)00277-X
Whyatt RM, Garfinkel R, Hoepner LA, Andrews H, Holmes D, Williams MK, Barr DB. 2009. A biomarker validation study of prenatal chlorpyrifos exposure within an inner-city cohort duringpregnancy. Environmental health perspectives 117(4), 559. https://doi.org/10.1289/ehp.0800041
Yousafzai AM. 2004. Toxicological effects of industrial effluents dumped in river kabul on mahaseer, Tor putitora at Aman Garh industrial area, nowshera, Peshawar, Pakistan (Doctoral dissertation, University Of Punjab) Pakistan.
Zama D, Meraihi Z, Tebibel S, Benayssa W, Benayache F, Benayache S, Vlietinck AJ. 2007. Chlorpyrifos-induced oxidative stress and tissue damage in the liver, kidney, brain and fetus in pregnant rats: The protective role of the butanolic extract of Paronychia argentea L. Indian Journal of Pharmacology 39(3), 145. https://doi.org.10.4103/0253-7613.33434
Baseerat Shaheen, Ali Muhammad Yousafzai (2019), Mollifying actions of garlic (Allium sativum) against Chloropyrifos induced toxicity by using rabbit (Oryctolagus cuniculus) as a model subject; IJB, V15, N1, July, P129-143
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