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Mollifying actions of garlic (Allium sativum) against Chloropyrifos induced toxicity by using rabbit (Oryctolagus cuniculus) as a model subject

Baseerat Shaheen, Ali Muhammad Yousafzai

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Int. J. Biosci.15(1), 129-143, July 2019

DOI: http://dx.doi.org/10.12692/ijb/15.1.129-143


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Potency of garlic is being acknowledged for years and is frequently used as a remedy for different diseases. In developing countries, the widespread use of pesticides in daily life has caused severe environmental pollution and consequently health hazards, with cases of severe acute and chronic poisoning in organisms. Recently, a growing interest has been shown into utilizing the biological activities of different medicinal herbs against diseases, due to their natural origin, lesser side effects and cost effectiveness. The current study was designed to evaluate the therapeutic role of garlic against the Chloropyrifos (CPF) induced toxicity in male rabbits (Oryctolagus cuniculus). For this purpose, male healthy rabbits (N=24) were procured from the local market and were divided into eight groups on the basis of acute and chronic toxicity evaluations. Group “A” and A1 were left untreated and were considered as the control group for acute and chronic toxicity   trials respectively. Group “B” and B1 were the Garlic fed group while group “C” and C1 were the Chloropyrifos (CPF) treated group, while group “D” and D1 were garlic co Chloropyrifos (CPF) administered group.  Rabbits were orally administered with doses of garlic and CPF for a period of 14 and 60 days on daily basis for evaluating acute and chronic toxicity respectively.  At the end of the trials, blood samples were collected in tubes for investigation of the hematological indices. Hematological analyzer (Sysmex KX-21 N, USA made) was used for analysis. The tested parameters showed a variability in values in comparison to the untreated group. In the acute toxicity experiment, a significant percent decrease in the level of Hb (9.83%), RBCs (16.54%), WBCs (20.64%), HCT(12.63%), Plts(5.5%) were observed in pesticide treated group while in garlic fed group the level of Hb (13.44%), RBCs (15.8%) ,WBCs (29.8%), HCT(21.5%), Plts (15%) showed % increase as compare to CPF and Control groups. In CPF co- Garlic administered group, garlic has attenuated the toxic effects of CPF. In conclusion CPF is found to be hematotoxic in nature both in acute and chronic doses and Garlic was found to be protective against its toxicity hence reflecting its importance. Therefore it could be advised as an effective dietary supplement in the developing countries where the use of pesticide is high.


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Mollifying actions of garlic (Allium sativum) against Chloropyrifos induced toxicity by using rabbit (Oryctolagus cuniculus) as a model subject

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