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Morpho-Biochemical characterization of cutaneous bacterial isolates of three endemic frogs from Mindanao Island, Philippines

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Research Paper 01/07/2018
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Morpho-Biochemical characterization of cutaneous bacterial isolates of three endemic frogs from Mindanao Island, Philippines

Elsa May D. Baron, Vanessa L. Calimbo, Sheryl Tanguanco, Christine Young, Boyeth Pelone, Pebe Ahinga, Rizza Lumangco, Lorelie Gloria A. Samaniego
Int. J. Micro. Myco.7( 4), 35-40, July 2018.
Certificate: IJMM 2018 [Generate Certificate]
Key: AnuransBiochemical testsCutaneous microbiotaEcological associationsMorphology

Abstract

We characterized through morphological features and some biochemical tests bacterial isolates from the skin of three endemic frog species: Kalophyrnus sinensis (Peters, 1867), Limnonectes magnus (Stejneger, 1910), and Megophrys stejnegeri (Taylor, 1920) from Mt. Andapon Barangay Campawan, in Baganga, Davao Oriental, Philippines. The bacterial isolates were acquired through skin swabs from five representative adult individuals per species, grown in select solid media, and subjected to various standard biochemical tests. Nine bacterial isolates were obtained: Citrobacter sp., Salmonella sp., Pseudomonas sp., Enterobacter sp., Micrococcus sp., Proteus sp., Staphylococcus sp., Staphylococcus aureus, and Diplococcus sp. Eight of these isolates were found in Megophrys stejnegeri (Taylor, 1920). Many of the bacterial isolates obtained were associated with soil. Citrobacter sp. was the common bacterial isolate found in all the frog species on both dorsal and ventral sides. The presence of bacterial isolates on these frogs maybe suggestive of a mutualistic relationship. Further studies maybe done to decipher role of these bacterial isolates and to validate if these are the only microorganism thriving on the skin of these Philippine endemic frogs.

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