Morphological and biochemical study of Bulgarian species Agaricus bohusii

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Morphological and biochemical study of Bulgarian species Agaricus bohusii

Maria Lacheva
J. Bio. Env. Sci.4( 3), 32-39, March 2014.
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Тhe study is first report on the biochemical composition of Agaricus bohusii Bon. The results obtained contribute to the knowledge of the chemical composition of mushrooms belonging to the Agaricus genus. There has been isolation, identification and analysis of the polar fraction, lipid and mineral content, as well as of pigments. Proteins contained 18 amino acids. Amino acid profile showed A. bohusii proteins are rich in valin, glutamic acid, aspartic acid, glycine, isoleucine and lysine. A detailed macro– and microscopic description is given. The species are described and illustrated on the basis of Bulgarian specimens.


Becker ZE. 1988. Physiology and Biochemistry of fungi. Moscow, Publishing House. Moscow University, 1- 229.

Bernando D, Mendoza C, Calonje M, Novaes-Ledieu M. 1999. Chemical analysis of the lamella walls of Agaricus bisporus fruit bodies. Current Microbiology 38(6), 364- 367.

Bohus G. 1971. Agaricus Studies III. Annales historico-naturales Musei nationalis hungarici 63, 77-82.

Bon M. 1983. Novitates. Validations de taxons et combinaisons nouvelles. Documents Mycologiques 13(49), p. 56.

Bernaš  E,  Jaworska  G,  Lisiewska  Z.  2006. Edible mushrooms as a source of valuable nutritive constituents. Acta Scientiarum Polonorum, Technologia Alimentaria 5, 5- 20.

Bonzom P, Nicolaou A, Eloh M, Baldeo W, Gibbons W. 1999. NMR lipid profile of Agaricus bisporus. Phytochemistry 50(8), 1311- 1321.

Britton G. 1983. The biochemistry of natural pigments. Cambridge University Press, 1- 421.

Brondz I, Hoiland K, Ekeberg D. 2004. Multivariatate analysis of fatty acids in spores of higher basidiomycetes: a new method for chemotaxonomical classification of fungi. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 800(1- 2), 303–307.

Buhalo AS. 1988. Higher edible Basidiomycetes in pure culture. Naukova Dumka. Kiev, 1- 144.

Cappelli A. 1983. Il genere Agaricus L. ex Fr. s. Karsten. Sezione “Rubescentes” dei bischi. Bollettino del Gruppo micologico G. Bresadola, Trento 26(1-2), 4- 38.

Cappelli A. 1984. Agaricus L.: Fr. ss. Karsten (Psalliota Fr.). Fungi Europaei. Libreria editrice Biella Giovanna. Saronno.

Chang S, Hayes W. 1978. The Biology and Cultivation of Edible Mushrooms. Academic Press, 1-840.

Dabbour I, Takruri HR. 2002. Protein Digestibility using Corrected Amino Acid Score method (PDCAAS) of four types of mushrooms in Jordan. Plant Foods for Human Nutrition 57(1), 13-24.

Didukh MY, Wasser SP, Nevo E. 2003. Medicinal value of species of the family Agaricaceae Cohn (Higher Basidiomycetes): Current stage of Knowledge and Future Perspecrives. International Journal of Medicinal Mushrooms 5, 133- 152.

Foret V, Arpin N. 1991. Space-time distribution of some soluble nitrogenous compounds of the Agaricus bisporus sporophore. Mushroom Science XII. Proceedings of the 13-th international congress on the science and cultivation of edible fungi. Dublin, Irish Republic, 1-6 September, 1991, p. 729- 735.

Gyosheva MM, Denchev CM, Dimitrova EG, Assyov B, Petrova RD, Stoichev GT. 2006. Red List of Fungi in Bulgaria. Mycologia Balcanica 3(1), 81- 87.

Kalač P. 2009. Chemical composition and nutritional value of European species of wild growing mushrooms: A review. Food Chemistry 113, 9- 16.

Kalač P. 2012. Chemical composition and nutritional value of European species of wild growing mushrooms. In: S. Andres and N. Baumann (Eds.). Mushrooms: Types, Properties and Nutrition, Nova Science Publisher, Inc, 1- 26.

Kamagai T. 1988. Photocontrol of fungal development. Photochemistry and Photobiology 47(6), 889 896.

Klyshev  LK,  Bandyukova  VA,  Alyukina  LS. 1978. Flavonoids are plant (distribution, physical and chemical properties, methods of research). Alma-Ata: Nauka, p. 220.

Lacheva M N. 2006. Genus Agaricus L.: Fr. emend. P. Karst. (mushroom) in Bulgaria – taxonomy, ecol-ogy, chorology and economical importance. PhD thesis, Agricultural University-Plovdiv,Bulgaria. [].

Lacheva M. 2011a. Rare and threatened taxa of Agaricus in Bulgaria. In: E. Ivanova [ed.]. Jubilee National Scientific Conference with International Participation “The man and the universe”, Smolyan, 6-8 Oct 2011, Union of Scientists in Bulgaria, Proceedings, 625–632 p.

Lacheva M. 2012c. New data of some rare larger fungi of Agaricaceae (Agaricales) inBulgaria. Science and Technologies 2(6), 24- 29.

Lacheva M. 2014. New data about Agaricales in Bulgaria. Second Anniversary Scientific Conference on Ecology, 25 years Department of Ecology and Environmental Conservation, 1st November 2013, Plovdiv, Bulgaria. (In press.)

Lowry OH, Rosenbrough HJ, Farr AL, Randal RJ. 1951. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193(1), 265-275.

Marh AT, Krzhevova RV. 1962.  Chemical  and technical control of canning production, Pishtepromizdat, 1- 435.

Oleshko VS, Babickaya VG. 1991. Amino acid and fractional composition of proteins offungal origin. Mycology and Phytopathology 25(3), 233- 239.

Parra LA. 2005. Nomenclatural study of the genus Agaricus L. (Agaricales, Basidiomycotina) of the Iberian Peninsula and Balearic Islands. Cuadernos de Trabajo de Flora Micolόgica Ibérica 21, 1- 101.

Pedneault K, Angers P, Gosselin A, Tweddell RJ. 2008. Fatty acid profilles of polar and neutral lipids of ten species of higher basidiomycetes indigenous to eastern Canada. Mycological Research 112, 1428- 1434.

Peev D et al. (Eds). 2011. Red Data Book if the Republic of Bulgaria. Vol. 1. Plant andFungi. IBEI-BAS & MOEW, Sofia.

Petrova A, Alipieva K, Kostadinova E, Antonova D, Lacheva M, Gyosheva M, Popov S, Bankova V. 2007. GC–MS studies of the chemical composition of two inedible mushrooms of the genus Agaricus. Chemistry Central Journal 1, 33-37.

Reis FS, Stojković D, Soković M, Glamočlija J, Ćirić A, Barros L, Ferreira I. 2012. Chemical characterization of Agaricus bohusii, antioxidant potential and antifungal preserving properties when incorporated in cream cheese. Food Research International 48(2), 620- 626.

Ribeiro B, Lopes R, Andrade PB, Seabra RM, Gonçalves RF, Baptista P, Quelhas I, Valentão P. 2008a. Comparative study of phytochemicals and antioxidant potential of wild edible mushroom caps and stipes. Food Chemistry 110, 47- 56.

Ribeiro B, Andrade PB, Baptista P, Quelhas I, Valentão P. 2009. Fatty acid composition of wild edible mushroom species: A comparative study. Microchemical Journal 93, 29- 35.

Sawickii AN. 1967. Determination of tryptophan content in yeast. Microbiological synthesis 7, 12- 15.

Schäeffer J, Møller FH. 1938. Beitrag zur Psalliota Forschung. Annales Mycologici Editi in Notitiam Scientiae Mycologicae Universalis 36, 64- 82.

Shnyukova EI. 1994. Algologiya 4(3), 29- 35.

Staheev IV. 1978. Cultivation of yeast and fungi producing protein on potato processing waste, Minsk, 1978.

Sudina EG. 1978. Biochemistry algae. Naukova Dumka. Kiev, 1- 228.

Uzun Y, Gençcelep H, Tunçtürk Y, Demirel K. 2009. Determination of protein and nitrogen fractions of wild edible mushroom. Asian Journal of Chemistry 21, 2769- 2776.

Velíšek J, Cejpek K. 2011. Pigments of higher fungi: A review. Czech Journal of Food Sciences 29, 87- 102.

Wasser SP, Shnyukova EI, Brun GO, Mushak PO, Sivash OO, Los SI, Fomishyna RM. 1999. Morphological and biochemical characteristics of Agaricus geesteranii Bas & Heinem. (Agaricales s.l., Basidiomycotina). Ukrainian Botanical Journal 56(6), 588- 592.

Wasser SP, Brun GO, Shnyukova EI, Mushak PO, Sivash OO, Los SI, Fomishyna RM. 2001. Biochemical characteristics of Agaricus geesteranii Bas & Heinem. (Agaricales s.l., Basidiomycotina). International Journal of Medicinal Mushrooms 3(2-3), 246.

Wasser SP, Weis AL. 1999. Medicinal properties occurring in higher Basidiomycetes mushrooms: current perspectives (revivew). International Journal of Medicinal Mushrooms 1, 31 62.

Wasser SP, Nevo E, Sokolov D, Reshetnikov SV, Timor-Tismenetsky M. 2000. Dietary supplements from medicinal mushrooms: diversity of types and variety of regulations. International Journal of Medicinal Mushrooms 2(1), 1- 18.

Websait: ODONAT (Coord.) 2003. Les listes rouges de la nature menacée en Alsace. Version électronique