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Morphological and molecular analysis of commercial Cordyceps militaris strains in Thailand

Junya Nopparat, Kawee Sujipuli, Wassana Chatdumrong

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Int. J. Biosci.13(4), 378-386, October 2018

DOI: http://dx.doi.org/10.12692/ijb/13.4.378-386


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Cordyceps militaris, an entomopathogenic fungusi, has been extensively utilized for many years as a functional food and for medicinal activities in Thailand. The fungus has numerous strains which are commonly sold in herbal markets and not all have been identified. Seven commercial C. militaris strains (CMs1, CMs2, CMs3, CMs4, CMs5, CMs6 and CMs7) were studied using macroscopic methods (mycelium growth), microscopic methods (cross section and SEM techniques), and molecular markers. Most strains recorded high mycelial growth rates at colony diameters of 7.81±0.20 to 8.50±0.00cm after 18 days, with strain CMs4 the lowest at 6.24±0.19cm. Characteristics of the stromal cross section in all strains observed under a microscope were golden yellow at the edge with colorless hyphae. A few conidia of all strains observed by SEM had oval shapes but most had globose shape with more single than short chain conidia. Genetic diversity among the seven strains gave 100% nucleotide-sequence homology at the ITS and 28S rRNA regions. These data suggested that the macroscopic method was a highly powerful tool for strain identification of C. militaris.


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Morphological and molecular analysis of commercial Cordyceps militaris strains in Thailand

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