Morphological and molecular characterization of three fungi strains isolated from locals foods in Burkina Faso
Paper Details
Morphological and molecular characterization of three fungi strains isolated from locals foods in Burkina Faso
Abstract
In this study we aims to characterize three fungi isolates S1, S2 and S3respectively collected from maize, groundnuts and rice. These isolates have antibacterial activities against two (2) Gram negative and two (2) Gram positive bacteria. Firstly identification was performed using phenotypic methods including macroscopic colony characteristics and microscopic morphology by identification key. Secondly, molecular methodwas used by two (2) PCR based on 28S ribosomal sub unit (D1-D2 region) and the hyper variable ITS1-5.8S-ITS2 region.The morphological characterization of the isolates has been confirmed by molecular characterization. Phylogenetic trees obtained allowed us to clearly identify and classify the isolates. Indeed, according to phylogenetic trees fungi isolates S1, S2 and S3 collected from local food in Burkina Faso were in the same clusters to those whose sequences are from toGenBank. As indicated by the results, isolates S1, S2 and S3 are respectively Aspergillus fumigatus, Aspergillus flavus and Penicillium citrinum.
Bruce WJ, Brian LW, Les MH. 2006.Direct genomic DNA sequencing for rapid fungal identification. Epicentre Biotechnologies Forum13 (4),8-13.
Chen YC, Eisner JD, Kattar MM, Rassoulian-Barrett SL, Lafe K, Bui U, Limaye AP, Cookson BT. 2001. Polymorphic internal transcribed spacer region 1 DNA sequences identify medically important yeasts. Journal of Clinical Microbiology39, 4042-4051.
Compaoré H, Sawadogo-Lingani H, Guira F, Samandoulougou S, Savadogo A, Dianou D, Traoré SA. 2016a.Enhancement of antibacterial compounds production by Aspergillus flavus and Penicillium citrinum isolated from locals foods in Bobo Dioulasso and Ouahigouya, Burkina Faso. European Journal of Pharmaceutical and Medical Research3(10),354-363. http://www.ejpmr.com/home/current_issues
Compaoré H, Sawadogo-Lingani H, Guira F, Waré LY, Samandoulougou S, Savadogo A, Dianou D, Traoré SA. 2016b. Optimization of antimicrobial compound production by Aspergillus fumigatus isolated from maize in Ouagadougou, Burkina Faso. Current Research in Microbiology and Biotechnology4(4), 903-911. http://crmb.aizeonpublishers.net/content/2016/4/crmb903-911.pdf
Cooney DG, Emerson R. 1964.Thermophilic fungi ed. W.H. Freeman and Company. An account of their biology, activities and classification.
Frisvad JC, Bridge PD, Arora DK. 1998.Chemical fungal taxonomy ed. Marcel Dekker: New York.
Goltapeh EM, Aggarwal R, Pakdaman BS, Renu. 2007. Molecular characterization of Aspergillus species through Amplicon Length Polymorphism (ALP) using universal rice primers. Journal of Agricultural Technology3(1),29-37.
Gonzalez A, Hierro N, Poblet M, Mas A, Guillamon JM. 2005. Application of molecular methods to demonstrate species and strain evolution of acetic acid bacteria population during wine production. International Journal of Food Microbiology, 102, 295-304.
Gouy M, Guindon S, Gascuel O. 2010. SeaView version 4: A multiplatform graphical user interface for sequence alignment and phylogenetic tree building. Molecular Biology and Evolution27(2), 221-224.
Kurtzman CP. 1993. DNA-DNA hybridization approaches to species identification in small genome organisms. Methods in Enzymology, 224, 335-348.
La Guerche S, Gracia C, Darriet P, Dubourdieu D, Labarère J. 2004.Characterization of Penicillium species isolated from grapes berries by their internal transcribed spacer (ITS1) sequences and by gas chromatography-mass spectrometry analysis of geosmin production. Current Microbiology,48(6), 405-411.
Lamrani K. 2009. Etude de la biodiversité des moisissures nuisibles et utiles isolées à partir des Maâsra du Maroc. Thèse de Doctorat d’Etat, Université Mohamed V- Agdal, Rabat, 213 p.
Lamrani K, Lakhtar H, Ismaili Alaoui M, Ettalibi M, Boiron P, Augur C, Gaime Perraud I, Roussos S. 2008.Production of fumagillin by Aspergillus fumigatus isolated from traditional trituration units, ”Maasra”, in Morocco Micologia Aplicada International20(1), 35-41.
Nguyen M. 2007. Identification des espèces de moisissures, potentiellement productrices de mycotoxines dans le riz commercialisé dans cinq provinces de la région centrale du vietnam – étude des conditions pouvant réduire la production des mycotoxines. Thèse de Doctorat, Université de Toulouse, Toulouse, 147 p.
O’Donnell K. 1992.Ribosomal DNA internal transcribed spacers are highly divergent in the phytopathogenic Ascomycete Fusarium sambucinum (Gibberella pulicaris). Current Genetique22, 213-220.
Ouattara-Sourabie PB, Nikiema PA, Traore AS. 2011. Caractérisation de souches d’Aspergillusspp isolées des graines d’arachides cultivées au Burkina Faso, Afrique de l’Ouest. International Journal of Biological and Chemical Science 5(3), 1232-1249. http://ajol.info/index.php/ijbcs
Patino B, Gonzalez-Salgado A, Gonzalez-Jaen MT, Vazquez C. 2005. PCR detection assays for the ochratoxin-producing Aspergillus carbonarius and Aspergillus ochraceus species. International Journal of Food Microbiology 104(2),207-214.
Pitt JI. 1985. The genus Penicillium ed. Academic Press. 634 p.
Raper KB, Fennell DI. 1965. The Genus Aspergillus ed. The Williams and Wilkins Company. 686 p.
Schoch CL, Seifert KA, Huhndorf S. 2012. Nuclear ribosomal Internal Transcribed Spacer (ITS) region as a universal DNA barcode marker for Fungi. Proceeding of the National. Academyof Sciences of the United States of America109, 6241-6246. www.pnas.org/cgi/doi/10.1073/pnas.1117018109
White TJ, Bruns Lee S, Taylor T. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In “PCR protocols: A guide to methods and applications”. Innis N, Gelfland J, White T, ed. New York. Academic Press. p. 315-322.
Hamidou Compaore, Hagrétou Sawadogo-Lingani, Larissa Yacine Ware, Filbert Guira, Serge Samandoulougou, Aly Savadogo, Alfred S. Traore (2016), Morphological and molecular characterization of three fungi strains isolated from locals foods in Burkina Faso; IJB, V9, N6, December, P374-382
https://innspub.net/morphological-and-molecular-characterization-of-three-fungi-strains-isolated-from-locals-foods-in-burkina-faso/
Copyright © 2016
By Authors and International
Network for Natural Sciences
(INNSPUB) https://innspub.net
This article is published under the terms of the
Creative Commons Attribution License 4.0