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Research Paper | December 1, 2018

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Morphological and molecular identification of Monosporascus cannonballus causal agent of melon root rot and plant decline in Iraq

Hanan W. Najem, T. A. Kareem

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J. Bio. Env. Sci.13(6), 83-88, December 2018

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Abstract

The study was conducted to characterize the causal agent of root rot and melon vine decline based on morphological characteristics and at molecular level. Pieces of melon infected roots were surface sterilized in 1% sodium hypochlorite and placed on Potato Dextrose Agar ( PDA) in petri plates and the fungi grown were purified and identified . Based on morphological characteristics six isolate belong to Monosporascus cannonballus, Mono1 – Mono6 were identified . The isolates Mono1 – Mono4 were found highly pathogenic to radish and melon seedlings with discoloring root length between 4.45 – 5 mm. The morphological characteristics were confirmed by PCR amplification of isolates DNA using specific and general primers. The analysis of PCR amplification products by agarose gel electrophoresis revealed bands of 112bp long when using specific primers , 600bp with general ITS1/ITS4 primers. The neighbor – joining phytogenetic tree and sequence comparison with M. cannonballus isolates in Gen Bank revealed high identify ( Up to 99% ) with the isolates from Tunis, Spain, China and clustered in the same group. The Iraqi isolates were recorded in Gen Bank under accession numbers MH179067 – MH179070.

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Morphological and molecular identification of Monosporascus cannonballus causal agent of melon root rot and plant decline in Iraq

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