Morphological and molecular identification of Monosporascus cannonballus causal agent of melon root rot and plant decline in Iraq
Paper Details
Morphological and molecular identification of Monosporascus cannonballus causal agent of melon root rot and plant decline in Iraq
Abstract
The study was conducted to characterize the causal agent of root rot and melon vine decline based on morphological characteristics and at molecular level. Pieces of melon infected roots were surface sterilized in 1% sodium hypochlorite and placed on Potato Dextrose Agar ( PDA) in petri plates and the fungi grown were purified and identified . Based on morphological characteristics six isolate belong to Monosporascus cannonballus, Mono1 – Mono6 were identified . The isolates Mono1 – Mono4 were found highly pathogenic to radish and melon seedlings with discoloring root length between 4.45 – 5 mm. The morphological characteristics were confirmed by PCR amplification of isolates DNA using specific and general primers. The analysis of PCR amplification products by agarose gel electrophoresis revealed bands of 112bp long when using specific primers , 600bp with general ITS1/ITS4 primers. The neighbor – joining phytogenetic tree and sequence comparison with M. cannonballus isolates in Gen Bank revealed high identify ( Up to 99% ) with the isolates from Tunis, Spain, China and clustered in the same group. The Iraqi isolates were recorded in Gen Bank under accession numbers MH179067 – MH179070.
Abolfazl S, Vadieh, Mohammed R. 2012. Detection of Monosporascus cannonballus from melon plants using PCR. J. Crop Prot. 1(4), 349-359.
Alendri MP, Martigononi D, Reda R, Chilosi G. 2017. Effect of Preconditioning Through Mycorrhizal Inoculation on the Controlof Melon Root Rot and Vine Decline Caused by Monosporascus cannonballus. Phytopathology Journal (163), 898-907.
Ben SI, Correia KC, Boughalleb N, Michereff SJ, Leon M, Abad-Campos P, Garcia-Jimenz J, Armengol J. 2013. M. eutypoides, acause of root rot and vine decline in Tunisia, and evidence that M. cannonballus and M. eutypoides are distinct species. Plant Dis. 97, 737-743.
Cintia DSB, Kamila CC, Marcos PSC, Rui SJ, Josep A, Sami JM. 2013. Population structure of Monosporascus cannonballus isolated from melons produced in Northeastren Brazil based on mycelium from compatibility groups. Acta Scientiarum 35, 161-167.
Cluck TW, Biles CL, Duggen M, Jackson T, Carson K, Armengol J, Garcia-Jimenez J, Bruton BD. 2009. Association of dsRNA to Down Regulation of Perthecia Synthesis in Monosporascus cannonballus The Open Mycology Journal 3, 9-19.
Guo Y, Liu L, Zhao J, Bi Y. 2007. Use of silicon oxide and sodium silicate for controlling Trichothecium roseum postharvest rot in Chinese cantaloupe ( Cucumis melo L. ). International Journal of Food Science Technology 42(8), 1012-1018.
Martyn RD. 2002. Monosporascus root rot and vine decline of melons. The Plant Helth nstructor, http://dx.doi.org/10.1094/PH-I-2002-0612-01.
Siguenza C, Schochow M, Turiniand T, Ploeg A. 2005 . Use of Cucumis metuliferus as aroot stock for melon to manage Meloidogyne incognita J Nematol 37(3), 276–280.
Sneh B, Yamoah E, Stewart A. 2004. Hypovirulent Rhizoctonia spp Isolates from New Zealand soils protect Radish seedling against damping – off caused by R. solani. New Zealand Plant Protection Society (57), 54–58.
Stanghellini ME, Kim DM, Waugh MM, Ferrin DM, Alcanatara T, Rasmussen SL. 2004. Infection and colonization of melon by Monosporascus cannonballus in Arizona and California. Plant Pathology 53, 54-57.
Hanan W. Najem, T. A. Kareem, 2018. Morphological and molecular identification of Monosporascus cannonballus causal agent of melon root rot and plant decline in Iraq. J. Biodiv. Environ. Sci., 13(6), 83-88.
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