Morphological characterization and differential virulence of various Pakistani isolates of Fusarium oxysporum f. sp. tuberosi

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Morphological characterization and differential virulence of various Pakistani isolates of Fusarium oxysporum f. sp. tuberosi

Sania Ahmed, Abid Riaz, Abdul Rauf, M. Kauser Nawaz Shah
Int. J. Biosci.12( 5), 63-71, May 2018.
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Fusarium wilt of potato caused by Fusarium oxysporum is a soil borne fungus that causes huge losses to the potato growers in Pakistan. Limited information is available about this pathogen. Therefore, a survey was conducted to different areas of Punjab to collect the disease samples and they were morphologically identified on the basis of size and shape of microconidia, macroconidia and chlamydospores. Microconidia were oval shaped with no septation whereas macroconidia were straight in shape that were slight curved at apex having 3-5 septations. Pathogenicity test was performed on susceptible potato variety Desiree. Out of 43 isolates, three isolates showed high virulence i.e. W8 (70.80%), S1 (81.75%) and O1 (84.38%). Yield reduction by highly virulent isolates ranged from 59.92 % to 76.26 %.  Ten isolates showed moderately virulent response W7 (53.08 %), O3 (55.76 %), TS1 (53.85 %), TS2 (56.14 %), BM3 (53.60 %), FD1 (44.35 %), CT2 (43.00%), O5 (43.0%), N1 (41.81%), and O2 (49.53 %). Yield reduction by moderately virulent isolates ranged from 45.58 to 52.42 %.  Thirteen isolates viz. FD2, BM1, CT3, N2 , C1, C2, T2, B3, B4 , B1, B2, S5 and W4  yield reduction ranged from 9.64 % to 28.59 %.  Three isolates were highly virulent, 5 exhibited  moderately virulent and rest of the isolates were avirulent. The recent study will help for efficient management of the pathogen through host plant resistance.


Amini  J, Sidovich D. 2010. The Effects of Fungicides on Fusarium oxysporum f. sp. Lycopersici Associated with Fusarium Wilt of Tomato. Journal of Plant Protection Research 50,172-178.

Ahmad B, Baksh K, Hassan S. 2004. Agricultural Economics and R.S., University of Agriculture Faisalabad, Pakistan. A report submitted to Pakistan Agriculture Research Council (PARC) Islamabad, Pakistan.

Altaf R, Rauf Ch A, Naz F, Shabbir G. 2014. Surveillance and morphological characterization of Fusarium isolates associated with lentil wilt. Pakistan Journal of. Phytopathology 26, 85-90.

Arslanoglus F, Aytac S, Oner KE. 2011. Morphological characterization of the local potato (Solanum tuberosum L.) genotypes collected from the Eastern Black Sea region of Turkey. African Journal of Biotechnology 10,922-932.

Ayed F, Remadi MD, Khiareddine JH, Mahjoub ME.2006a. Potato Vascular Fusarium wilt in Tunisia. Incidence and control by Trichoderma. Plant Pathology 5, 92-98.

Bhutta AR. 2008. Survey of tuber-borne diseases of potato in Northern areas, Pakistan. Pakistan Journal of Phytopathology 21, 20-37.

FAOSTAT.(online). 2015. Food and Agriculture Organization of the United Nations – Rome, Italy.

Fiers  M,  Edel-Hermann V, Chatot C,  Le-Hingrat Y, Alabouvette C, Steinberg C. 2012.  Potato soil-borne diseases. A review. Agronomy for Sustainable Development32,93-132.

Leslie JF, Summerell BA. 2006. The Fusarium laboratory manual. Wiley- Blackwell Publishing Professional, Ames, IA, USA, 388 p.

Nauman- Etienne K.1990. Current situation and future strategy in seed potto production. PSPDP / PARC Report, P.O. 1031, Islamabad.

Naz F, Rauf CA, Abbasi NA, Haque I, Ahmad I.2008. Influence of innoculum levels of Rhizoctonia solani and susceptibility on new potato germplasm , Pakistan  Journal of Botany 40, 2199-2209.

Nelson PE, Toussoun TA, Marasas WFO. 1983. Fusarium species: an illustrated manual for identification, 1ST ed. University Park (PA): Pennsylvania state university Press, 1-5 p.

Pakistan Agriculture Research Council. 1998. Annual technical reports of potato project, autumn crop. 1996, 1997. PARC, IBP. Pakistan, 1-235 p.

Perkins DD. 1962. Preservation of Neurospora stock cultures with anhydrous silica gel. Canadian Journal of  Microbiology 8,591-594.

Santosh FMA, Romas B, Sanchez MAG, Eslava AP, Minguez JMD. 2002. A DNA based procedures for in Planta detection of Fusarium oxysporum f. sp. phaseoli. Phytopathology 92, 237-244.

Silva  JC, Bettol W. 2005. Potential of non-pathogenic Fusarium oxysporum isolate for  control of fusarium wilt of tomato. Fitopatologia Brasileria 30, 409-412.

Toussoun TE, Nelson PE. 1976. A pictorial guide to the identification of Fusarium species. Second edition.The Pennsylvania State University Park.USA, 43 p.

Turkensteen. 1992. Survey of potato diseases in control of black scurf and other potato tubers with green crop harvest and antagonistic organisms. In: G. Wzehder, M. L. powelson, P. K. Jansson and K.V. Raman (eds). Advances in potato pest biology and management. APS Press St. Paul M.N. USA. 325-331.

Windels CE, Burnes PM, Kommedahl MT. 1988. Five year preservation of Fusarium species on silica gel and soil . Phytopathology 78, 107-109.

Zaheer  Z, Shafique S, Shafique S, Mehmood T. 2012. Evaluation of pathogenic potential and genetic characterization of Fusarium solani: A cause of Fusarium wilt of potato. African Journel of Biotechnology 6, 1762-1765.