Morphological, meristic characteristics and mtDNA analysis of Hampala Fish (Hampala macrolepidota Kuhl & Van Hasselt 1823) from ranau lake, Indonesia

Paper Details

Research Paper 01/08/2014
Views (304) Download (18)
current_issue_feature_image
publication_file

Morphological, meristic characteristics and mtDNA analysis of Hampala Fish (Hampala macrolepidota Kuhl & Van Hasselt 1823) from ranau lake, Indonesia

Safran Makmur, Diana Arfiati, Gatut Bintoro, Arning Wilujeng Ekawati
J. Bio. Env. Sci.5( 2), 447-455, August 2014.
Certificate: JBES 2014 [Generate Certificate]

Abstract

Hampala (Hampala macrolepidota) in Ranau Lake is locally known by three size-based names: small size as Kemencut, medium as Arongan and large as Sebarau. To know whether these local names are the same or different species, morphometric measurements, meristic counts and mtDNA analysis were carried out. Five individuals of each size, 157–172 mm total length for Kemencut, 264–295 mm for Arongan and 374–445 mm for Sebarau were taken. Morphometric measurements showed significant correlation (p > 0.01). The strongest correlation between body part and percent of total length was predorsal length (PL) with r = 0.980. Meristic character counts fit to the identification of Weber and Beaufort (1916). Number of scales along the lateral line and total number of pectoral fin were 27-28 and 15-16, respectively. Nucleotide base composition of H. macrolepidota did not show any variation in nucleotide situs. Intraspecific COI (Cytochrome Oxidase Subunit I) gene nucleotide-based phylogram of Hampala created a genetic relationship supported by boostrap value of 100%. No haplotypic variation was formed. All Hampala samples were included in H. macrolepidota (Kuhl & Van Hasselt 1823).

VIEWS 19

Abbaspour R, Rahbar M, Karimi JM. 2013. Comparative survey of morphometric-meristic male and female anjak fish (Schizocypris brucei, Annandale and Hora, 1920) of Hamoun Wetland in South East Iran. Middle-East Journal of Science Research 14, 620–623. http://dx.doi.org/ 10.5829/idosi.mejsr.2013.14.5.73148.

Alo D, Correa C, Arias C, Cordenas L. 2013. Diversity of Aplichiton fishes (Galaxiidae) and taxonomic resurrection of A. marinus. Plos One Italy 8 (e71577), 1–11.

Anisimova M, Gascuel O. 2006. Approximate likelihood ratio test for branchs: a fast, accurate and powerful alternative. Systematic Biology 55, 539–552.

Cailet GM, Love MS, Ebeling AW. 1986. Fishes: a field and laboratory manual on their structure identification and natural history. California: Wadsworth Publishing Company Belmont.

Castresana J. 2000. Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Molecular Biology and Evolution 17, 540–52.

Dereeper A, Guignon V, Blanc G, Audic S, Buffet S, Chevenet F, Dufayard JF, Guindon S, Lefort V, Lescot M, Claverie JM, Gascuel O. 2008. Phylogeny.fr: robust phylogenetic analysis for the  non-specialist. Nucleic  Acids  Research  1, 465–469. http://dx.doi.org/10.1093/nar/gkn180.

Doi A, Taki Y. 1994. A new Cyprinid fish, Hampala salweenensis, from the Mae Pae River System, Salween Basin, Thailand. Japanese Journal of Ichthyology 40, 405–412.

Gharaei A. 2012. Morphometric and meristic studies of snow trout Schizothorax zarudnyi (Nikolskii, 1897) as a threatened endemic fish. World Journal of Fish and Marine Sciences 4, 426–429. http://dx.doi.org/10.5829/idosi.wjfms.2012.04.04.63123.

Guindon S, Dufayard JF, Lefort V, Anisimova M, Hordijk W, Gascuel O. 2010. New algorithms and methods to estimate Maximum-Likelihood Phylogenies: assessing the performance of PhyML 3.0. Systematic Biology 59, 307–21. http://dx.doi.org/10.1093/sysbio/syq010.

Hazarika A, Borah U, Bordoloi L. 2011. Studies on morphometric measurements and meristic counts of Hill Trout (Barilius bendelisis, Hamilton) from the River Buroi at the boundary areas of Assam and Arunachal Pradesh, India. Indian Journal of Fundamental and Applied Life Science 1, 194–198.

Hossain  MdY,  Ohtomi  J,  Ahmed  ZF.  2009. Morphometric, meristic characteristics and conservation of the threatened fish, Puntius sarana (Hamilton, 1822) (Cyprinidae) In the Gangges River, Northwestern Bangladesh. Turkish Journal of Fisheries and Aquatic Science 9, 223–225. http://dx.doi.org/10.4194/trjfas.2009. 0215.

Katoh K, Mizawa K, Kuma K, Miyata T. 2002. Mafft: a novel method for rapid multiple sequence alignment basedon fast furier transform. Nucleic Acid Research 30, 3059–3066.

Kottelat M, Whitten AJ, Kartikasari SN, Wiroatmodjo S. 1993. Freshwater fishes of Western Indonesia and Sulawesi. Indonesia: Periplus Editions Ltd.

Krishan NR, Tarana N. 2010. Analysis of morphometric characters of Schizothorax richardsonii (Gray, 1832) from the Uttarkashi District of Uttarkhand State, India. Biological Sciences 10, 536–540. http://dx.doi.org/10.3923/jbs.2010.536.540.

Meyer CP, Paulay G. 2005. DNA bar coding: Error rates based on comprehensive sampling. PLOS Biology 3, 2229–2238. http://dx.doi.org/10.1371/journal.pbio. 0030122.

Muladno. 2006. Molecular technological application in animal productivity development effort. In: Molecular diagnostic technical training for livestock and fisheries production development in eastern Indonesia. Bogor: Collaboration of Life Science Study Centre, Research Institution and Community Enpowerment IPB, and Directorate General of Higher Education, Dept. National Education.

Ryan JRJ, Esa YB. 2006. Phylogenetic analysis of Hampala fishes (Subfamily Cyprininae) in Malaysia inferred from partial mitochondrial cytochrome b DNA squences. Zoological Sciences 23, 893–901. http://dx.doi.org/10.2108/zsj.23.893.

Sulastri, Badjoeri M, Sudarso Y, Syawal MS. 1999. Physico-chemical and biological condition of Ranau Lake waters, South Sumatera. Research and Development Centre of Limnology, Indonesia Science Institute. Limnotek VI, 25–38.

Tegelstrom H. 1986. Mitochondrial DNA in natural populations: an improved routine for the screening of genetic variation based on sensitive silver staining. Electrophoresis 7, 226–229. http://dx.doi.org/10.1002/elps.1150070508.

Turan C, Oral M, Öztürk B, Düzgüneş E. 2006. Morphometric and meristic variation between socks of Bluefish (Pomatomus saltatrix) in the black, Marmara, Aegean and Northeastern Mediterranean Seas. Fisheries Research 79, 139–147.

Weber M, DeBeaufort LF. 1916. The fishes of Indo-Australian Archipelago III. Ostariophysi: II Cyprinoidea, Apodes, Synbranchi. Leiden: E.J. Brill Ltd., 455.

Yang L, Mayden RL, Sado T, He S, Saitoh K, Miya M. 2010. Molecular phylogeny of the fishes traditionally referred to Cyprinini sensu scripta (Teleostei: Cypriniformes). Zoologica Scripta. The Norwegian Academy of Science and Letters 39, 527–550. http://dx.doi.org/10.1111/j.1463-6409.2010.00443.x.

Zafar M, Nazir A, Akhtar N, Naqvi SMHM, Rehman M.Z. 2002. Studies on meristic and morphometric of Mehseer (Tor putitora) from a spawning ground of Himalayan foot-hill river Korang Islamabad, Pakistan. Pakistan Journal of Biological Sciences 5, 733–735.