Morphological variability survey of cyanobacterium Anabaena sp. ISC55 in liquid mixotrophe treatment

Paper Details

Research Paper 01/08/2014
Views (242) Download (21)

Morphological variability survey of cyanobacterium Anabaena sp. ISC55 in liquid mixotrophe treatment

Ehteram Deylami, Taher Najadsattari, Younes Ghasemi, Shadman Shokravi
J. Bio. Env. Sci.5( 2), 77-84, August 2014.
Certificate: JBES 2014 [Generate Certificate]


The variety of phenotypes of cyanobacteria is accompanied by morphological plasticity changing accordingly to different environmental or culture conditions. In this research, the effect of different treatments of liquid mixotrophe on morphological responses, structural changes and biometrical of Anabaena sp. ISC55 were studied. This investigation was performed in time periods of weekly and daily. Due to the morphological variability of cyanobacteria in different conditions, molecular and genetics studies should be performed for identify of this cyanobacterium. In this study identification of Anabaena sp. ISC55 was performed based on diacritical morphological such as cell shape, cell size, presence or absence of sheath and 16S rRNA gene sequencing. The results indicated that shape and size of vegetative cells and heterocytes in this species have changed in different treatments of liquid mixotrophe compared with control treatment; also this species in glucose treatment has produced more biomass.


Anagnostidis K, Komarek J. 1990. Modern approaches to the classification of Cianobacteria. Stigonematales. Archiv für Hydrobiologie, 14(8), 224-286.

Andersen RA. 2005.  Algal  culturing  techniques. USA: Academic press.

Bennet A, Bogorad L. 1973. Complimentary chromatic adaptation in a filamentous blue-green alga. Journal of Cell Biology 58, 419–425.

Bird DF, Kalff J. 1987. Algal phagotrophy: regulating factors and importance relative to photosynthesis in Dinobryon (Chrysophyceae). Limnol. Oceanogr 32, 277–284.

Blankenship, RE. 1992. Origin and early evolution of photosynthesis. Photosynthesis Research 33, 91-111.

Bockstahler KR, Coats DW. 1993. Grazing of the mixotrophic dinoflagellate Gymnodinium sanguineum on ciliate populations of Chesapeake Bay. Marine Biology 116, 477– 487.

Brocks, JJ, Logan GA, Buick R, Summons RE. 1999. Archean Molecular Fossils and the Early Rise of Eukaryotes. Science 285, 1033-1036

Case RJ, Boucher Y, Dahllof I, Holmstrom C, Doolittle WF, Kjelleberg S. 2007. Use of 16S rRNA and rpoB genes as molecular markers for microbial ecology studies. Applied and Environmental Microbiology 73, 278–288.

Cloern JE, Dufford R. 2005. Phytoplankton community ecology: principles applied to San Francisco Bay. Marine Ecology Progress Series, 285, 11–28.

Desikachary TV. 1959. Cyanophyta. Indian council of agricultural research. Monographs on Algae, New Delhi, India.

Ducat DC, Way JC, Silver PA. 2011. Engineering cyanobacteria to generate high-value products. Trends in Biotechnology 29(2), 95-103.

Geitler L. 1932. Cyanophyceae von Europa Kryptogamen flora Akademiche Verlagsgesellschaft. Leipzig

Gillis M, Vandamme P, De Vos P, Swings J, Kersters K. 2005. Polyphasic taxon-omy. In DJ Brenner, NR Krieg, JT Staley, eds, Bergey’s Manual of System-atic Bacteriology. The Proteobacteria, Vol. 2A, 2nd ed. Springer-Verlag, New York, USA, 43-48 P.

Grossman AR, Bhaya D, He Q. 2001. Tracking the light environment by cyanobacteria and the dynamic nature of light harvesting. Journal of Biological Chemistry 276, 11449–11452.

Han D, Fan Y, Hu Z. 2009. An evaluation of four phylogenetic markers in Nostoc: Implications for cyanobacterial phylogenetic studies at the intrageneric level. Current Microbiology 58, 170–176.

Hayes PK, El Semary NA, Sa´nchez-Baracaldo P. 2007. In Brodie, J. & J. Lewis (eds), Unravelling The Algae: The Past, Present, and Future of Algal Systematics. CRC Press/Taylor & Francis Group, Boca Raton, 93–101.

John DM, Whitton BW, Brook AJ. 2002 The freshwater algae of the British Isles, Cambridge University Press.

Knoll AH. 2008. Cyanobacteria and earth history. In Hererro A & Flores E (Eds.) The Cyanobacteria: Molecular Biology, Genomics and Evolution. Cais- ter Academic Press, Norfolk, UK, 1-20 P.

Komárek J. 2010. Modern taxonomic revision of planktic nostocacean cyanobacteria: a short review of genera. Hydrobiologia 639, 231–243.

Latała A, Misiewicz S. 2000. Effects of light, temperature and salinity on the growth and chlorophyll-a content of Baltic cyanobacterium Phormidium sp. Arch. Hydrobiol. 136, Algological Studies 100, 157–180.

Li R, Wilhelm SW, Carmichael WW, Watanabe MM. 2008. Polyphasic characterization of water bloom forming Raphidiopsis species (cyanobacteria) from central China. Harmful Algae 7, 146–153.

Lyra C, Suomalainen S, Gugger M, Vezie C, Sundman P, Paulin L, Sivonen K. 2001. Molecular characterization of planktic cyanobacteria of Anabaena, Aphanizomenon, Microcystis and Planktothrix genera. International Journal of Systematic and Evolutionary Microbiology 51, 513-526.

Maranger R, Bird DF, Price NM, 1998. Iron acquisition by photosynthetic marine phytoplankton from ingested bacteria. Nature 396, 248–251.

Nayak S, Prasanna R, Prasanna BM, Sahoo DB. 2007. Analysing diversity among Indian isolates of Anabaena (Nostocales, Cyanophyta) using morphological, physiological and biochemical characters. World Journal of Microbiology and Biotechnology 23,1575–1584.

Nübel U, Garcia-Pichel F, Kühl M,  Muyzer G. 1999. Quantifying microbial diversity: Morphotypes, 16S rRNA genes, and carotenoids of oxygenic phototrophs in microbial mats. Applied and Environmental Microbiology, 422-430.

Nübel U, Garcia-Pichel F, Clavero E, Muyzer G. 2000. Matching molecular diversity and ecophysiology of benthic cyanobacteria and diatoms in communities along a salinity gradient. Environmental Microbiology 2, 217-26.

Ostroff CR, Karlander EP, Van Valkenberg SD. 1980. Growth rates of Pseudopendinella pyriformis (Chrysophyceae) in response to 75 combinations of light, temperature, and salinity, Journal of Phycology 16,421–423.

Pan X, Chang F, Kang L, Li G, Li D, Liu Y, Shen Y, Wei Z. 2008. Morphological characteristics and phylogenetic relationship of Anabaena species from Lakes Dianchi and Erhai, China. Hydrobiologia 614, 353-362.

Prescott GW.1962. Algae of the western Great Lakes area, W.C. Brown Company publishers.

Rajaniemi P, Hrouzek P, Kastovská K, Willame R, Rantala A, Hoffmann L, Komárek J, Sivonen K. 2005. Phylogenetic and morphological evaluation of the genera Anabaena, Aphanizomenon, Trichormus and Nostoc (Nostocales, Cyanobacteria). International Journal of Systematic and Evolutionary Microbiology 55, 11–26.

Rippka R, Deruelles J, Waterbury JB, Herdman M, Stanier RY. 1979. Generic assignments, strain histories and properties of pure cultures of Cyanobacteria. Journal of General Microbiology 111, 1–61.

Saker M, Moreira C, Martins J, Neilan B, Vasconcelos VM. 2009. DNA profiling of complex bacterial populations: toxic cyanobacterial blooms. Applied Microbiology and Biotechnology 85, 237–252.

Sanders RW, Porter KG, Caron DA. 1990. Relationship between phototrophy and phagotrophy in the mixotrophic chrysophyte Poterioochromonas malhamensis. Microbial Ecology 19, 97–109.

Sanders RW. 1991. Mixotrophic protists in marine and freshwater ecosystems. Journal of Protozoology. 38, 76-81.

Schirrmeister BE, Dalquen DA, Anisimova M, Bagheri HC. 2012 Gene copy number variation and its significance in cyanobacterial phylogeny. BMC Microbiolgy 12(1), 177.

Schleifer KH. 2009. Classification of Bacteria and Archaea: past, present and future. Systematic & Applied Microbiology 32, 533–542.

Stibor H, Sommer U. 2003. Mixotrophy of a photosynthetic flagellate viewed from an optimal foraging perspective. Protist 154, 91–98.

Tandeau de Marsac N. 2003. Phycobiliproteins and phycobilisomes: the early observations. Photosynthesis Research 76, 197–205.

Vigaya V, Narayanaswamy A. 2009. Blue light enhance the pigment synthesis in cyanobacterium Anabaena ambigua rao(Nostacales). ARPN Journal of Science and Technology 4(3), 36-43.

Willame R, Boutte C, Grubisic S, Wilmotte A, Hoffmann L. Komárek J. 2006. Morphological and molecular characterization of planktonic cyanobacteria from Belgium and Luxembourg. Journal of Phycology 42, 1312–1332.

Zapomeˇlova´ E, Rehakova K, Jezberova J, Komarkova J. 2010. Polyphasic characterization of eight planktonic Anabaena strains (Cyanobacteria) with reference to the variability of 61 Anabaena populations observed in the field. Hydrobiologia 639, 99-113.