Morphometric contribution and molecular markers for the characterization and identification of olive tree (Olea europaea L.) varieties

Paper Details

Research Paper 01/02/2016
Views (387) Download (9)
current_issue_feature_image
publication_file

Morphometric contribution and molecular markers for the characterization and identification of olive tree (Olea europaea L.) varieties

M. Laib, Z. Djerrou, M.M. Bentchikou
J. Bio. Env. Sci.8( 2), 337-345, February 2016.
Certificate: JBES 2016 [Generate Certificate]

Abstract

This study focused the variability of morphological types and polymorphism level in olive tree. Ten SSR microsatellite markers and 23 morphological descriptors were used to characterize 15 indigenous cultivars of this species growing in Algeria. A significant morphological variability was observed between the cultivars. The type of the endocarp exhibited a discrimination potential higher than that of the fruit, leaf and the inflorescence. Molecular markers have also shown a very high level of polymorphism and a high discriminatory power to discriminate easily the whole sample. It is possible to achieve the same results using only 3 markers (ssr Oc UADCA 18, UDO 099-043 and ssrOcUA-DCA9). These results underline the importance of using ssr markers and morphological descriptors for the inventory and identification of olive varieties.

VIEWS 12

Alba V, Sabetta W, Blanco A, Pasqualone A, Montemurro C. 2009. Microsatellite markers to identify specific alleles in DNA extracted from monovarietal virgin olive oils. European Food Research and Technology 229, 375–382.

Bandelj D, Akse J, Javornik B. 2002. DNA fingerprinting of olive varieties by microsatellite markers. Food Technology and Biotechnology 40, 185–190.

Barranco D, Cimato A, Fiorino P, Rallo L, Touzani A, Castañeda C, Trujillo I. 2000. World catalogue of olive varieties. Intl. Olive Oil Council. Madrid, Spain.

Bartolini G, Prevost G, Messeri C, Carignani C. 2005. Olive Germplasm: Cultivars and world-Wide Collection. FAO, Rome, Italy.

Belaj A, Cipriani G, Testolin R, Rallo L, Trujillo I. 2004. Characterization and identification of the main Spanish and Italian olive cultivars by simple sequence-repeat markers. Hort Science 39, 1557-1561.

Belaj A, Satovic Z, Rallo L, Trujillo I. 2002. Genetic diversity and relationships in olive (Olea europaea L.) germplasm collections as determined by randomly amplified polymorphic DNA. Theoretical and Applied Genetics 105, 638-644.

Belaj A, Trujillo I, De La Rosa R, Rallo L. 2001. Polymorphism and Discrimination Capacity of Randomly Amplified Polymorphic Markers in an olive Germplasm Bank. Journal of the American Society for Horticultural Science 126(1), 64-71.

Benzecri JP. 1982. Construction d’une classification ascendante hiérarchique par la recherche en chaine des voisins réciproques. Cahiers de l’Analyse des Données 7, 209-218.

Besnard G, Baradat P, Bervillé A. 2001. Genetic relationships in the olive (Olea europaea L.) reflect multilocal selection of cultivars. Theoretical and Applied Genetics 102, 251–258.

Besnard G, Khadari B, Villemur P, Bervillé A. 2000. Cytoplasmic male sterility in olive (Olea europaea L.). Theoretical and Applied Genetics 100, 1018–1024.

Bracci T, Sebastiani L, Bosconi M, Fogher M, Belaj A, Trujillo I. 2009. SSR markers reveal the uniqueness of olive cultivars from the Italian region of Liguria. Scientia Horticulturae 122, 209-215.

Charafi J, El Meziane A, Moukhli A, Boulouha B, El Modafar C, Khadari B. 2008. A Moroccan olive germplasm collection identified by a SSR locus-based genetics study. Genetic Resources and Crop Evolution 55, 893-900.

Chaux C. 1955. Méthodes de recherches adoptées en matière de biologie florale de l’olivier. Fruits et primeurs de l’Afrique du nord 25, 202-207.

COI. 1997. Métrologie pour la caractérisation primaire des variétés d’oliviers. Projet RESGEN-CT (67/97), Union Européenne/Coi.

De LA Rosa R, James CM, Tobutt KR. 2002. Isolation and Characterization of polymorphic microsatellites in olive (Olea Europaea L.) and their transferability to other genera in the Oleaceae. Molecular Ecology Notes 2, 265-267.

Grati-Kamoun N, Lamy Mahmoud Reba F, Gargouri A, Panaud O, Saar A. 2006. Genetic diversity of Tunisian olive tree (Olea europaea L.) cultivars assessed by AFLP markers. Genetic Resources and Crop Evolution 53, 265–275.

Khadari B, Charafi J, Moukhli A, Ater M. 2008. Substantial genetic diversity in cultivated Moroccan olive despite a single major cultivar: a situation evidenced by the use of SSR loci. Tree Genetics & Genomes 4, 213-221.

Mendil M, Sebai A. 2006. Catalogue des Variété Algériennes de l’Olivier. Ministère de l’agriculture et du développement rural, ITAF Alger, Algeria. 98 p.

Nei M. 1977. F- Statistics and analysis of gene diversity in subdivided populations. Annals of Human Genetics 41, 225-233.

Ouazzani N, Lumaret R, Villemur P. 1996. Genetic variation in the olive tree (Olea europaea L.) cultivated in Morocco. Euphytica 91, 9–20.

Rekik I, Salimonti A, Kammoun NG, Lepais O, Gerber S, Perri E, Rebai A. 2008. Characterization and identification of Tunisian olive tree varieties by microsatellite markers. Hort Science 43, 1371–1376.

Sarri V, Baldoni L, Porceddu A, Cultrera NGM, Contento A, Frediani M, Belaj A, Trujillo I, Cionini PG. 2006. Microsatellite markers are powerful tools for discriminating among olive cultivars and assigning them to geographically defined populations. Genome 49, 1606–1615.

Taamalli W, Geuna F, Bassi D, Daoud D, Zarrouk M. 2008. SSR marker based DNA fingerprinting of Tunisian olive (Olea europaea L.) varieties. Journal of Agronomy 7, 176–181.

Trujillo I, Rallo L, Arús P. 1995. Identifying olive cultivars by isozyme analysis. Journal of the American Society for Horticultural Science 120, 318– 324.

Ward JH. 1963. Hierarchical grouping to optimize an objective function. Journal of American Statistical Association 58, 236–244.