Mortality response of wheat aphid (Rhopalosiphum padi) against most commonly used insecticides in Pakistan

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Research Paper 01/02/2016
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Mortality response of wheat aphid (Rhopalosiphum padi) against most commonly used insecticides in Pakistan

Muhammad Saleem Khan, Naureen Aziz Quershi, Muhammad Usman Khan, Farhat Jabeen, Muhammad Shakeel, Ali Umar, Muhammad Saleem Asghar
Int. J. Biosci.8( 2), 1-8, February 2016.
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Rhopalosiphum padi is one of the most common wheat inhabiting pest and reduce the crop yield up to `15%. This pest species was collected from the wheat fields located in the vicinity of Faisalabad. The mortality response of said pest was find out in the different insecticides by standard nymphal dip method. The results showed that LC50 values of 9 tested insecticides against R. padi are statically different (5% probability level) and varied from 1.33±0.52 ppm for Imidacloprid to119.17± 16.55 ppm for Charphyrifos. Imidacloprid which caused 100% mortality at 64, 32 and 16 ppm more than 80% mortality at 8, 4 and 2 ppm was proved to be the most toxic insecticide against the R. padi. The Charphyrifos was least toxic as it caused only 41.53±3.21% mortality even at highest concentration. This study recommended the minimum dose recommendation in ppm for controlling this damaging insect. This study could be used as a reference for resistance monitoring or other related researches.


Akhtar  HI,  Khaliq  A.  2003.  Impact of plant phenology and coccinellid predators on the population dynamic of rose aphid Macrosiphum rosaeiformis das (Aphididae: Homoptera) on rose. Asian Journal of Plant Sciences (Pakistan). 01/2003;2.

Brault V, Herrbach ÉReinbold C. 2007. Electron microscopy studies on luteovirid transmission by aphids. Micron 38(3), 302-312.

Ghafoor A, Khan MS, Mahmood A, Ahmad F, Hassan M, Anees A. 2011. Resistance status of Helicoverpa armigera (Hub) against mixture of Profenofos and Indoxacarb (1:1) insecticides at Faisalabad, Pakistan. World Applied Sciences Journal 13(1), 90-94.

Ghafoor A, Saba I, Khan MS, Farooq HA, mjad I. 2011. Predatory potential of Cryptolaemus montrouzieri for Cotton Mealybug under laboratory conditions. JAPS, Journal of Animal and Plant Sciences 21(1), 90-93.

Goggin FL. 2007. Plant–aphid interactions: molecular and ecological perspectives. Current opinion in plant biology 10(4), 399-408.

Huang S, Xu JHan Z. 2006. Baseline toxicity data of insecticides against the common cutworm Spodoptera litura Fabricius and a comparison of resistance monitoring methods. International Journal of Pest Management 52(3), 209-213.

Joshi N, Sharma V. 2009. Efficacy of imidacloprid (confidor 200 SL) against aphids infesting wheat crop. Journal of Central European Agriculture 10(3), 217-221.

Khan AM, Khan AA, Afzal M, Iqbal MS. 2012. Wheat crop yield losses caused by the aphids infestation. J Biofertil Biopestici 3(122), 2.

Khan MS, Asghar MS, Maqsood I, Bukhari M, Han L, Li-jie T, Yi-jing L, Shahla AKhalil U. 2015. Laboratory Observations Regarding Different Instars of Cyclosainsulana (Costa, 1834) (Araneidae) During Developmental Stages. Journal of Northeast Agricultural University (English Edition) 22(2), 52-59. http://10.1016/S1006-8104(15)30032-5.

Khan SA, Ullah F, Hussain N, Saljoqi A, Hayat Y, Sattar S. 2006. Distribution pattern of the cereal aphids in the wheat growing areas of the North West Frontier Province (NWFP) of Pakistan. Sarhad Journal of Agriculture 22(4), 655.

Khattak MK, Riazuddin, Anayatullah M. 2007. Population dynamics of aphids (Aphididae: Homoptera) on different wheat cultivars and response of cultivars to aphids in respect of yield and yield related parameters. Pakistan Journal of Zoology 39(2), 109-115.

Khuhro R, Ghafoor A, Mahmood A, Khan MS, Andleeb S, Bukhari M, Maqsood I, Shahjahan M, Baloch N. 2012. Assessment of potential of predatory spiders in controlling the cotton jassid (Amrasca devastans) under laboratory conditions. The Journal of Animal and Plant Sciences 22(3), 635-638.

Kibe A, Singh S, Kalra N. 2006. Water–nitrogen relationships for wheat growth and productivity in late sown conditions. Agricultural Water Management 84(3), 221-228.

Kuroli G. 2000. Aphid flight and change in abundance of winter wheat pests. Archives of Phytopathology & Plant Protection 33(4), 361-373. http://dx.doi./10.1080/03235400009383356.

Memon RA, Bhatti GR, Khalid S, Mallah A, Ahmed S. 2013. Illustrated weed flora of wheat crop of Khairpur District, Sindh. Pakistan Journal of Botany 45(1), 39-47.

Mohsin SB, Li Yi-jing TL-j, Maqsood I, Ting Ma-sun, Han Le-meng, Khalil U R, Andleeb S, Khan MS, Asghar SM. 2015. Predatory Efficacy of Cotton Inhabiting Spiders on Bemisiatabaci, Amrascadevastans Thripstabaci and Helicoverpa armigera in Laboratory Conditions. Journal of Northeast Agricultural University (English Edition) 22(3), 48-53.

Mushtaq S, Rana SA, Khan HA, Ashfaq M. 2013. Diversity and abundance of family aphididae from selected crops of Faisalabad, Pakistan. Pakistan Journal of Agriculture Science 50(1), 103-109.

Oerke EC. 2006. Crop losses to pests. The Journal of Agricultural Science 144(01), 31-43.

PAR. (2015). Pakistan Wheat Production. from

Pathak L, Saxena R, Sharma H. 2013. Haematological changes in major Indian carps under stress of malathion and parathion. Indian Journal of Biological Research and studies 2(2), 112-120.

Rossing W, Daamen R, Jansen M. 1994. Uncertainty analysis applied to supervised control of aphids and brown rust in winter wheat. Part 2. Relative importance of different components of uncertainty. Agricultural systems 44(4), 449-460. http://doi:10.1016/0308-521X(94)90197-N

Sadeghi A, Van Damme EJ, Smagghe G. 2009. Evaluation of the susceptibility of the pea aphid, Acyrthosiphon pisum, to a selection of novel biorational insecticides using an artificial diet. Journal of Insect science 9(1), 65.

Taheri S, RazmJou J, Ra STegaRi N. 2010. Fecundity and development rate of the bird cherry-oat. Plant Protection Science 46, 72-78.

Tang LD, Wu JH, Ali S, Ren SX. 2013. Establishment of baseline toxicity data to different insecticides for Aphis craccivora Koch and Rhopalosiphum maidis (Fitch)(Homoptera: Aphididae) by glass tube residual film technique. Pakistan Journal of Zoology 45(2), 411.

Yanhui L, Ting Y, XiWu G. 2009. Establishment of baseline susceptibility data to various insecticides for aphids Rhopalosiphum padi (Linnaeus) and Sitobion avenae (Fabricius)(Homoptera: Aphididae) by the method of residual film in glass tube. Acta Entomologica Sinica 52(1), 52-58.

Zhang P, Zhang X, Zhao Y, Wei Y, Mu W, Liu F. 2015. Effects of imidacloprid and clothianidin seed treatments on wheat aphids and their natural enemies on winter wheat. Pest Management Science. 2015 Aug 7.

Zhu KY, Gao JR, Starkey SR. 2000. Organophosphate resistance mediated by alterations of acetylcholinesterase in a resistant clone of the greenbug, Schizaphis graminum (Homoptera: Aphididae). Pesticide Biochemistry and Physiology 68(3), 138-147.