Welcome to International Network for Natural Sciences I INNSpub

Paper Details

Research Paper | November 1, 2018

| Download 1

Mycoendophytes as pest control agents, using culture filtrates, against green apple aphid; Aphis pomi (Hemiptera, Aphididae)

Khamssa Rouabah, Nadia Lombarkia, Oussama Ali Bensaci, Tunali Berna

Key Words:

Int. J. Biosci.13(5), 16-26, November 2018

DOI: http://dx.doi.org/10.12692/ijb/13.5.16-26


IJB 2018 [Generate Certificate]


Aphis pomi is a significant pest of apples. Chemical pesticides have been the practical method used for the control of economically important pest insects. Today, it is recognized that the use of constantly increasing pesticides in agriculture, constitute a serious problem in different fields. Why, the recourse to alternative strategies of control is considered like one of the most promising solutions. Biological control using endophytic fungi is a promising alternative to chemical control. The purpose of the research was to determine the effectiveness of some isolates of endophytic fungi, for the control of the green apple aphid. The study consisted of four isolated treatments applied as culture filtrates pure bioformulations against Aphis pomi at different concentrations (25%, 50%, 75% and 100%), where mortality rate was determined. To show a possible relationship between aphicid enzymatic activities, protease enzyme was quantified and proteolytic index was determined. Results showed that fungal filtrates having a variable aphicid activity. We recorded variable mortality rates according to the type of filtrates and the gradient of concentration where Trichoderma sp. had the best activity. Thus, the evolution of mortality is not more proportional in relation to the gradient of concentration; it is rather about a maximal mortality (91, 76%) that corresponds to an optimal concentrations (25% and 100%). We noticed that the colony diameter and the proteolytic activity are two parameters correlated negatively; this relation is not expressed in the same way for fungi that have recorded a proteolytic activity. All fungal filtrates have an aphicid activity towards A. pomi with a negative correlation between colony diameter and proteolytic activity detected in isolates.


Copyright © 2018
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Mycoendophytes as pest control agents, using culture filtrates, against green apple aphid; Aphis pomi (Hemiptera, Aphididae)

Abbott WS. 1925. A method of computing the effectiveness of an insecticide. Journal of Economic Entomology 18, 265- 267.

Anderson CMT, Mcgee PA, Nehl DB, Mensah RK. 2007. The fungus Lecanicillium lecanii colonises the plant Gossypium hirsutum and the aphid Aphis gossypii. Australasian Mycologist 26, 65- 70.

Athman SY, Dubois T, Viljoen A, Labuschagne N, Coyne D, Ragama P, Gold CS, Niere B. 2006. In vitro antagonism of endophytic Fusarium oxysporum isolates against the burrowing nematode Radopholus similis. Nematology 8, 627- 636.

Azevedo JL, Maccheroni JRW, Pereira JO, Dearaujo WL. 2000. Endophytic microorganisms: a review on insect control and recent advances on tropical plants. Electronic Journal of Biotechnology 3, 40- 65.

Batta YA. 2004. Control of rice weevil (Sitophilus oryzae L., Coleoptera: Curculionidae) with various formulations of Metarhizium anisopliae. Crop Protection 23, 103- 108. http://dx.doi.org/10.1016/j.cropro.2003.07.001.

Belloncik S, Parent N. 1976. Toxicity of entomopathogenic fungi Cordyceps militaris against larvae of Culicidae. Entomophaga 21, 343- 347. (in French).

Bensaci OA, Daoud H, Lombarkia N, Rouabah K. 2015. Formulation of the endophytic fungus Cladosporium oxysporum Berk. & M.A. Curtis, isolated from Euphorbia bupleuroides subsp. luteola, as a new biocontrol tool against the black bean aphid (Aphis fabae Scop.). Journal of Plant Protection Research 55, 80- 87. http://dx.doi.org/10.1515/jppr-2015-0011.

Bermek H, Gulseren I, Li K, Jung H, Tamerler C. 2004. The effect of fungal morphology on ligninolytic enzyme production by a recently isolated wood-degrading fungus Trichophyton rubrum LSK-27. World Journal of Microbiology and Biotechnology 20, 345- 349.

Bharathidasan R, Panneerselvam A. 2011. Biodiversity of the endophytic fungi isolated from Avicennia marina in Ramanathapuram district, Karankadu. World Journal of Science and Technology 1, 01- 05.

Budiarto BR, Mustopa AZ, Tarman K. 2015. Isolation, purification and characterization of extracellular protease produced by marine-derived endophytic fungus Xylaria psidii KT30. Journal of Coastal Life Medicine 3, 56- 63. http://dx.doi.org/10.12980/JCLM.3.2015.APJTB-2014-0364.

Butt TM, Goettel MS. 2000. Bioassays of entomogenous fungi. In: Navon A,  Ascher KRS, Ed. Bioassays of Entomopathogenic Microbes and Nematodes. CAB International, Wallingford, UK. 141- 195.

Chougule NP, Bonning BC. 2012. Toxins for transgenic resistance to Hemipteran pests. Toxins 4, 405- 429. http://dx.doi.org/10.3390/toxins4060405.

Fattah FA, Webster JM. 1989. Ultra structural modifications of Meloidogyne javanica induced giant cells caused by fungal culture filtrates. Revue Nematology 12, 197-210.

Glare T, Caradus J, Gelernter W, Jackson T, Keyhani N, Kohl J, Marrone P, Morin L, Stewart A. 2012. Have biopesticides come of age?. Trends in Biotechnology 30, 250- 258. http://dx.doi.org/10.1016/j.tibtech.2012.01.003.

Gratzner HG. 1972. Cell wal alterations associated with the hyperproduction of extracellular enzymes in Neurospora crassa. J. Bacteriol 111, 443- 446.

Harrison RL, Bonning C. 2010. Proteases as insecticidal agents. Toxins 2, 935- 953.

Hassan AEHA. 2007. Novel natural products from endophytic fungi of Egyptian medicinal plants-chemical and biological characterization. Doctoral thesis, University of Düsseldorf, Egypte, 16- 28.

Kim YM, Song HG. 2009. Effect of fungal pellet morphology on enzyme activities involved in phthalate degradation. The Journal of Microbiology 47, 420- 424.

Kucera M. 1980. Proteases from the fungus Metarhizium anisopliae toxic for Galleria mellonella larvae. Journal of Invertebrate Pathology 35, 304- 310.

Lacey LA, Frutos R, Kaya HK, Vail P. 2001. Insect pathogens as biological control agents: do they have a future?. Biological Control 21, 230- 248.

Lopez-Llorca LV, Carbonell T, Gomez-Vidal S. 2002. Degradation of insect cuticle by Paecilomyces farinosus proteases. Mycological Progress 1, 249- 256.

Lowery DT, Smirle MJ, Foottit RG, Beers EH. 2006. Susceptibilities of Apple aphid and Spirea aphid collected from apple in the Pacific Northwest to selected insecticides. Journal of Economic Entomology 99, 1369- 1374.

Mesquitta ALM, Lacey LA, Mercadier G, LeClant F. 1996. Entomopathogenic activity of a whitefly-derived isolate of Paecilomyces fumosoroseus (Deuteromycotina: Hyphomycetes) against the Russian wheat aphid, Diuraphis noxia (Hemiptera: Sternorryncha Aphididae) with the description of an effective bioassay method. European Journal of Entomology 93, 69- 75.

Monod M, Capoccia S, Lechenne B, Zaugg C, Holdom M, Jousson O. 2002. Secreted proteases from pathogenic fungi. International Journal of Medicinal Microbiology 292, 405- 419.

Moscoso IL, Rosato YB. 1987. Extracellular enzyme production by haploids, heterocaryons and diploids of Aspergillus nidulans. Applied in Microbiol Biotechnology 26, 365- 368.

Orlandelli RC, Almeida TT, Alberto RN, Polonio JC, Azevedo JL, Pamphile JA. 2015. Antifungal and proteolytic activities of endophytic fungi isolated from Piper hispidum Sw. Brazilian Journal of Microbiology 46, 359- 366. http://dx.doi.org/10.1590/S1517-838246220131042.

Raju DC, Thomas SM, Thomas SE. 2015. Screening for extracellular enzyme production in endophytic fungi isolation from Calophyllum inophyllum. L leaves. Journal of Chemical and Pharmaceutical Research 7, 900- 904.

Raviraja NS, Maria GL, Sridhar KR. 2006. Antimicrobial evaluation of endophytic fungi inhabiting medicinal plants of the Western Ghats of India. Engineering in Life Sciences 6, 515- 520.

Ritu A, Anjali C, Nidhi T, Sheetal P, Deepak B. 2012. Biopesticidal formulation of Beauveria bassiana effective against larvae of Helicoverpa armigera. Biofertilizers and Biopesticides 3, 1- 3. http://dx.doi.org/10.4172/2155-6202.1000120.

Robert A, Messing-Al-Aidroos K. 1985. Acid production by Metarhizium anisopliae: Effect on virulence against mosquitoes and on detection of in vitro amylase, protease, and lipase activity. Journal of Invertebrate Pathology 45, 9- 15.

Sanchez-Perez LC, Barranco-Florido JE, Rodriguez-Navarro S, Cervantes-Mayagoitia JF, Ramos-Lopez MA. 2014. Enzymes of entomopathogenic fungi, advances and insights. Advances in Enzyme Research 2, 65- 76.

Schinya R, Aiuchi D, Kushida A, Tanim, Kuramochi K, Koike M. 2008. Effects of fungal culture filtrates of Verticillium lecanii (Lecanicillium spp.) hybrid strains on Heterodera glycines eggs and juveniles. Journal of Invertebrate Pathology 97, 291-297.

Seye F, Ndiaye M, Faye O, Afoutou JM. 2012. Evaluation of entomopathogenic fungus Metarhizium anisopliae formulated with suneem (neem oil) against Anopheles gambiae s.l. and Culex quinquefasciatus adults. Malaria Chemotherapy, Control and Elimination 1, 1- 6.

Shakeri J, Foster H. 2006. Proteolytic activity and antibiotic production by Trichoderma harzianum in relation to pathogenicity to insects. Enzyme and Microbial Technology 1- 8.

Stekoll M, West CA. 1978. Purification and properties of an elicitor of castor bean phytoalexin from culture filtrates of the fungus Rhizopus stolonifer. Plant Physiology 61, 38- 45.

Tejesvi MV, Mahesh B, Nalini MS, Prakash HS, Kini KR, Subbiah V, Shetty HS. 2006. Fungal endophyte assemblages from ethno pharmaceutically important medicinal trees. Canadian Journal of Microbiology 52, 427- 435. http://dx.doi.org/10.1139/w05-143.

Vega FE, Goettel MS, Blackwell M, Chandler D, Jackson MA, Keller S, Koike M, Maniania NK, Monzon A, Ownley BH, Pell JK, Rangel DEN, Roy HE. 2009. Fungal entomopathogens: new insights on their ecology. Fungal Ecology 2, 149- 159.

Vega FE, Posada F, Aime MC, Pava-Ripoll M, Infante F, Rehner SA. 2008. Entomopathogenic fungal endophytes. Biological Control 46, 72- 82. http://dx.doi.org/10.1016/j.biocontrol.2008.01.008.

Yeo H. 2000. Mycoinsecticides for aphid management: A biorational approach. Doctoral thesis, Sutton Bonington Campus, University of Nottingham, 58- 68.

Zaki FA. 1994. Effect of culture filtrates of Paecilomyces lilacinus on Meloidogyne javanica. Nematology Mediterranean 22, 41- 43.

Zaki MJ. 1999. Effect of fungal culture filtrates on mortality and hatching of Meloidogyne javanica. Pakistan Journal of Biological Sciences 2, 161- 163.

Zheng Y, Xue QY, Xu LL, Xu Q, Lu S, Gu C, Guo JH. 2011. A screening strategy of fungal biocontrol agents towards Verticillium wilt of cotton. Biological Control 56, 209- 216.

Znidarsic P, Pavko A. 2001. The morphology of filamentous fungi in submerged cultivations as a bioprocess parameter. Food Technology and  Biotechnology 39, 237- 252.


Style Switcher

Select Layout
Chose Color
Chose Pattren
Chose Background