Natural Resistance of Avian Sera to Trypanosoma brucei brucei (Plimmer & Bradford)

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Natural Resistance of Avian Sera to Trypanosoma brucei brucei (Plimmer & Bradford)

Djieyep-Djemna Felicite, Nock Ishaya Haruna, Aken’Ova Thelma, Kogi Ezekiel, Djieyep Noundo Armand Claude
Int. J. Biosci.17( 6), 54-61, December 2020.
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This study evaluated the natural anti-Trypanosoma brucei brucei activity of sera from some birds viz: Broiler chicken (Gallus gallus domesticus), Shika brown chicken (Gallus gallus domesticus), Parent Stock White chicken (Gallus gallus domesticus), Parent Stock brown chicken (Gallus gallus domesticus), Guinea fowl (Numida meleagris), Domestic pigeon (Columba livia domestica), Goose (Plectropterus gambensis), Crowned crane (Balearica regulorum), Peacock (Pavo cristatus), Spotted eagle owl (Bubo africanus), Hooded vulture (Necrosyrtes monachus) and Buzzard (Buteo buteo). To ensure that the donor birds were not infected with trypanosomes, their blood samples were screened using the thick film and haematocrit centrifugation technique (HCT). Trypanosoma brucei brucei (Federe strain) was suspended in RPMI supplemented with FCS and the motility of the parasite was used as an index of viability after the addition of each test serum. Bird sera generally exhibited some degree of anti-Trypanosoma brucei brucei activities in vitro. Serum from broiler hen had the highest anti-Trypanosoma brucei brucei activity showing an inhibition index of 0.83, with a marked and significant reduction (P<0.05) in survival time of 5.00±0.00 hours compared with that of all the sera from other birds including the control (30.00±0.00 hours). The in vitro anti-trypanosomal activity in all the serum samples was cidal in nature and appeared to be mediated by xanthine oxidase (XO), except in the serum of buzzard. The highest anti-Trypanosoma brucei brucei activity in the serum of broiler hen also coincided with the highest XO content. This study shows that sera from the selected birds have innate immunity against T. brucei brucei.


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