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Research Paper | November 1, 2014

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New primer generated bacterial mapping and biofertilizing potentiality assessment of Pseudomonas sp. isolated from cowdung

Gopinath Rana, Arindam Adhikari, Tanusri Mandal

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Int. J. Agron. Agri. Res.5(5), 1-12, November 2014

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Abstract

Modern biofertilizer trend is to use of different plant growth-promoting microorganisms (PGPM), isolated from several sources, including rhizosphere. Here we evaluate the plant growth promoting ability of some fluorescent Pseudomonads, isolated from cow dung. Based on different biochemical tests, we select 7 isolates to observe their effect on Cicer arietinum (Bengal gram) seed germination and sprout growth promotion. A new Pseudomonas sp Strain TMGR (NCBI Accession Number JX094352) showed best effect with Vigor-index value 4470. 16s rRNA gene sequencing revealed that all isolates have 97-99% similarities with Pseudomonas aeruginosa. On the basis of Molecular Evolutionary Genetics Analysis version 6.0 (MEGA6), analysis of 16S rRNA sequences of selected seven isolates with other native Pseudomonas aeruginosa strains were available in GeneBank, a phylogenetic tree was constructed to study their relative position. Adopting BioEdit and Fast PCR software programs, we designed Pseudomonas aeruginosa specific two primers, 103F and 455R, and their validity was rechecked in wet laboratory. These primers may help in isolation and rapid identification of plant growth promoting Pseudomonas aeruginosa strains from different sources in future.

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New primer generated bacterial mapping and biofertilizing potentiality assessment of Pseudomonas sp. isolated from cowdung

Adhikari A, Rana G, Mandal T. 2014. An in silico assessment of molecular evolution at 16S rRNA of Pseudomonas sp. International Journal of Current Microbiology and Applied Sciences 3(8), 518-527.

Ahmadzadeh M, Afsharmanesh H, Javan-Nikkhah M, Sharifi-Tehrani A. 2006. Identification of some molecular traits in fluorescent pseudomonads with antifungal activity. Iranian Journal of Biotechnology 4(4), 245-253.

Chaiharn M, Chunhaleuchanon S, Kozo A, Lumyong S. 2008. Screening of rhizobacteria for their plant growth promoting activities. KMITL Science and Technology Journal 89(1), 18-23.

Chakraborty AK. 2014. Structure and functions of rodents ID retroposon located in the c-Ha-ras oncogene far upstream regulatory region. Current Science 107 (in press).

Fernandez LA, Zalba P, Gomez MA, Sagardoy MA. 2007. Phosphate solubilization activity of bacterial strains in soil and their effect on soybean growth under greenhouse conditions. Biology and Fertility of Soils 43, 805-809.

Glick BR, Changping L, Sibdas G, Dumbroff EB. 1997. Early development of canola seedlings in the presence of the plant growth-promoting rhizobacterium Pseudomonas putida GR12-2. Soil Biology and Biochemistry 29, 1233–1239.

Glick BR. 1995. The enhancement of plant growth by free-living bacteria. Canadian Journal of Microbiology 41, 109–117.

Hall JA, Pierson D, Ghosh S, Glick BR. 1996. Root elongation in various agronomic crops by the plant growth promoting rhizobacterium Pseudomonas putida GR12-2. Israel Journal of Plant Sciences 44, 37–42.

Henri F, Laurette NN, Annette D, John Q, Wolfgang M, François-Xavier E, Dieudonné N. 2008. Solubilization of inorganic phosphates and plant growth promotion by strains of Pseudomonas fluorescens isolated from acidic soils of Cameroon. African Journal of Microbiology Research 2, 171-178.

Jayaprakashvel M, Muthezhilan R, Srinivasan R, Hussain AJ, Gobalakrishnan S, Bhagat J, Kaarthikeyan C, Muthulakshmi R. 2010. Hydrogen Cyanide Mediated Biocontrol Potential of Pseudomonas sp. AMET1055 Isolated from The Rhizosphere of Coastal Sand Dune Vegetation. Advanced Biotech 9(10), 39 – 42.

Karnwal A. 2009. Production of indole acetic acid by fluorescent Pseudomonas in the presence of L-tryptophan and rice root exudates. Journal of Plant Pathology 91, 61-63.

Khan AA, Jilani G, Akhtar MS, Naqvi SMS, Rasheed M. 2009. Phosphorus solubilizing bacteria: Occurrence, mechanisms and their role in crop production. Journal of Agriculture and Soil Science 1, 48-58.

Kibbe WA. 2007. OligoCalc: an online oligonucleotide properties calculator. Nucleic Acids Research 35, 43-46.

King EO, Ward MK, Raney DE. 1954. Two simple media for the demonstration of pyocyanin and fluorescein. Journal of Laboratory and Clinical Medicine 44, 301-307.

Kumari BS, Ram MR, Mallaiah KV. 2009. Studies on exopolysaccharide and indole acetic acid production by Rhizobium strains from Indigofera. African Journal of Microbiology Research 3, 10-14.

Kwon SW, Kim JS, Crowley DE, Lim CK. 2005. Phylogenetic diversity of fluorescent pseudomonads in agricultural soils from Korea. Letters in Applied Microbiology 41, 417–423.

Lemanceau P. 1992. Effects benefiques de rhizobacteries sur les plantes: exemple des Pseudomonas spp. fluorescent. Agronomie 12, 413– 37.

Leong J. 1986. Siderophores: their biochemistry and possible role in the biocontrol of plant pathogens. Annual Review of Phytopathology 24, 187–208.

Mahdi SS, Hassan GI, Hussain A, Rasool F. 2011. Phosphorus Availability Issue- Its Fixation and Role of Phosphate Solubilizing Bacteria in Phosphate Solubilization. Research Journal of Agricultural Science 2(1), 174-179.

Manjunatha H, Naik MK, Patil MB, Lokesha R, Vasudevan SN. 2012. Isolation and characterization of native fluorescent pseudomonads and antagonistic activity against major plant pathogens. Karnataka Journal of Agricultural Science 25(3), 346-349.

Muhammad S, Amusa NA. 2003. In-vitro inhibition of growth of some seedling blight inducing pathogens by compost-inhabiting microbes. African Journal of Biotechnology 2(6), 161-164.

Murray MG, Thormpson WF. 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research 8, 4321–4326.

Naik PR, Raman G, Narayanan KB, Sakthivel N. 2008. Assessment of genetic and functional diversity of phosphate solubilizing fluorescent pseudomonads isolated from rhizospheric soil. BMC Microbiology 8, 1-14. http://dx.doi.org/10.1186/1471-2180-8-230

Oliveira CA, Alves VMC. 2008. Phosphate solubilizing microorganisms isolated from rhizosphere of maize cultivated in an oxisol of the Brazilian cerrado biome. Soil Biology and Biochemistry xx, 1-6.

Puente ME, Bashan Y, Li CY, Lebsky VK. 2004. Microbial populations and activities in the rhizoplane of rock-weathering desert plants. Root colonization and weathering of igneous rocks. Plant Biology 6, 629-642.

Punitha S, Balamurugan I, Kuberan T, Kumar RS. 2010. Isolation and characterization of agriculturally important microbes from panchakavya and their enzymatic activity. Journal of Biosciences Research 1(3), 194-201.

Ramamoorthy V, Raguchander T, Samiyappan R. 2002. Induction of defense-related proteins in tomato roots treated with Pseudomonas fluorescens Pf1 and Fusarium oxysporum f. sp. Lycopersici V. Plant and Soil 239, 55–68.

Rana G, Mandal T, Mandal NK. 2014. Generation of high calorific fuel gas by photosynthetic bacteria isolated from cowdung. International Journal of Research (IJR) 1(8), 2014.

Rana G, Mukherjee C, Mandal T, Mandal NK. 2013. Removal of fluoride ions from fluoride containing compounds using some bacteria. International Journal of Environment Pollution Control and Management 5(2), 68-71.

Rana G, Shome D, Mandal T, Mandal NK. 2012. Control of environment pollution by effective utilization of fly-ash, produced by thermal power plants, in cultivated lands by extracting its useful nutrients using pseudomonas bacteria isolated from cowdung. International Journal of Environment Pollution Control and Management 4(2), 147-152.

Reddy BP, Reddy KRN, Subha Rao M, Rao KS. 2008. Efficacy of Antimicrobial Metabolites of Pseudomonas fluorescens Against Rice Fungal Pathogens. Current Trends in Biotechnology and Pharmacy 2(1), 178-182.

Renninger N, Knopp R, Nitsche H, Clark, DS, Keasling JD. 2004. Uranyl Precipitation by Pseudomonas aeruginosa via Controlled Polyphosphate Metabolism. Applied and Environmental Microbiology 70(12), 7404–7412.

Rodriguez H, Fraga R, Gonzalez T, Bashan Y. 2006. Genetics of phosphate solubilization and its potential applications for improving plant growth promoting bacteria. Plant and Soil 287, 15-21. http://dx.doi.org/10.1007/s11104-006-9056-9

Rodriguez H, Fraga R. 1999. Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnology Advances 17, 319-339.

Roychoudhury P, Kaushik BD. 1989. Solubilization of Mussorie rock phosphate by cyanobacteria. Current Science 58, 569–70.

Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA. 1988. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239, 487-491.

Scarpellini M, Franzetti L, Galli A. 2004. Development of PCR assay to identify Pseudomonas fluorescens and its biotype. FEMS Microbiology Letters 236, 257–260.

Shahab S, Ahmed N, Khan NS. 2009. Indole acetic acid production and enhanced plant growth promotion by indigenous PSBs. African Journal of Agricultural Research 4, 1312-1316.

Sreenivasa MN, Naik N, Bhat SN. 2009. Beejamrutha: A source of beneficial bacteria. Karnataka Journal of Agricultural Science 22, 1038-1040.

Swain MR, Naskar SK, Ray RC. 2007. Indole-3-acetic acid production and effect on sprouting of yam (Dioscorea rotundata L.) minisetts by Bacillus subtilis isolated from culturable cowdung microflora. Polish Journal of Microbiology 56(2), 103-110.

Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. 2013. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution 30, 2725-2729.

Teo KC, Teoh SM. 2011. Preliminary biological screening of microbes isolated from cow dung in Kampar. African Journal of Biotechnology 10(9), 1640-1645.

Wasi S, Tabrez S, Ahmad M. 2013. Use of Pseudomonas spp. for the bioremediation of environmental pollutants: a review. Environment Monitoring and Assessment 185, 8147-8155.

Watanabe FS, Olsen SR. 1965. Test of an ascorbic acid method for determining phosphorous in water and NaHCO3 extracts from soil. Soil Science Society of American Proceedings 29, 677–678.

Weisburg WG, Barns MS, Pelletier AD, Lane JD. 1991. 16S ribosomal DNA amplification for phylogenetic study. Journal of Bacteriology 173, 697-703.

Wilson K. 1987. Preparation of genomic DNA from bacteria. In: Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K ed. Current Protocols in Molecular Biology, New York,241-242.

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