Nitrate reductase activity of seven local corn cultivars from south west maluku district during water stress caused by polyethylene glycol 6000 under green house condition

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Research Paper 01/07/2019
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Nitrate reductase activity of seven local corn cultivars from south west maluku district during water stress caused by polyethylene glycol 6000 under green house condition

Hermalina Sinay, Mariana S.J. Suripatty
Int. J. Biosci.15( 1), 80-86, July 2019.
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Nitrate reductase activity is one of indicators that always observed in the correlation with the plant adaptation toward drought condition. One of procedures that could be conducted to develop the drought-tolerant of corn is by the selection of dryness by in vitro on limited scale like in the laboratory or in a green house, with using polyethylene glycol (PEG). This research objective is to know the activity of nitrate reductase on local corn cultivar from Kisar island South West Maluku regency after using Polyethylene glycol (PEG 6000) in a green house. The methods of this research consist of several steps such as preparation of corn seed, cultivation on growing media, drought stress treatment with 10% of polyethylene glycol, and analysis of Nitrate Reductase activity (NRA). The data collected were then analyzed descriptively. The result shows that on all local corn cultivars, the nitrate reductase activity on control group (without drought stress treatment) is lower than the corn suffering the drought stress by the treatment using PEG 6000 10%. It can be conclude that highest nitrate reductase activity value is obtained on the corn cultivar wich was treated with drought stress   treatment using PEG 6000. The highest nitrate reductase activity is on Kuning Genjah cultivar (266.8 μmol/g), while the lowest one of nitrate reductase activity is on Merah Delima Tongkol Putih cultivar (81.36 μmol/g).


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