Welcome to International Network for Natural Sciences | INNSpub

Nitric oxide alleviates freezing stress by reducing oxidative injury in Citrumelo leaves

Research Paper | July 1, 2016

| Download 4

Mojdeh Nazarpour, Fariborz Zare Nahandi, Mohammad Reza Dadpour

Key Words:

J. Bio. Env. Sci.9( 1), 390-399, July 2016


JBES 2016 [Generate Certificate]


Freezing stress causes economical losses of citrus in the world. Nitric oxide could alleviate this stress. In this study the effects of sodium nitroprusside (SNP) as NO donor (0, 0.1 and 1 mM) on temperature treatments (+9, -3, -6 and -9°C) were evaluated. Application of 0.1 mM SNP decreased the electrolyte leakage and lipid peroxidation of Citrumelo leaves significantly. The highest antioxidant activity was detected in 0.1 mM SNP at -6 °C. Maximum content of total phenolics was at -9 °C and 0.1 mM SNP application. SNP also had positive effects on antioxidant enzymes activity. Superoxide dismutase, catalase and peroxidase had highest activity at -6°C with 0.1 mM SNP, and the highest activity of APX was in 0.1 mM SNP at -9°C. The results showed nitric oxide released from SNP increased the antioxidant capacity of Citrumelo leaves against freezing induced oxidative stress.


Copyright © 2016
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Nitric oxide alleviates freezing stress by reducing oxidative injury in Citrumelo leaves

Aebi H. 1984. Catalase in vitro. Methods in enzym-ology 105, 121-126.

Apel K, Hirt H. 2004. Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol 55, 373-399.

Asada K. 2006. Production and Scavenging of Reactive Oxygen Species in Chloroplasts and Their Functions. Plant Physiology. June 1, 2006; 141, 391-396.

Baek K-H, Skinner DZ. 2012. Production of reactive oxygen species by freezing stress and the protective roles of antioxidant enzymes in plants.

Baudouin E. 2011. The language of nitric oxide signalling. Plant Biology 13, 233-242.

Blum A, Ebercon A. 1981. Cell membrane stability as a measure of drought and heat tolerance in wheat. Crop Science 21, 43-47.

Böhm FMLZ, Ferrarese MdLL, Zanardo DIL, Magalhaes JR, Ferrarese-Filho O. 2010. Nitric oxide affecting root growth, lignification and related enzymes in soybean seedlings. Acta physiologiae plantarum 32, 1039-1046.

Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry 72, 248-254.

Brand-Williams W, Cuvelier M-E, Berset C. 1995. Use of a free radical method to evaluate antioxidant activity. LWT-Food science and Technology 28, 25-30.

Castle WS, Wutscher H, Youtsey C, Pelosi R. Citrumelos as rootstocks for Florida citrus. Proceedings of the Proc Fla State Hort Soc 1988.

Chance B, Maehly A. 1955. [136] Assay of catalases and peroxidases. Methods in enzymology 2, 764-775.

Corpas FJ, Chaki M, Fernández-Ocaña A, Valderrama R, Palma JM, Carreras A, Begara-Morales JC, Airaki M, del Río LA, Barroso JB. 2008. Metabolism of reactive nitrogen species in pea plants under abiotic stress conditions. Plant and Cell Physiology 49, 1711-1722.

Esim N, Atici O. 2014. Nitric oxide improves chilling tolerance of maize by affecting apoplastic antioxidative enzymes in leaves. Plant growth regulation 72, 29-38.

Fan Q-J, Liu J-H. 2012. Nitric oxide is involved in dehydration/drought tolerance in Poncirus trifoliata seedlings through regulation of antioxidant systems and stomatal response. Plant cell reports 31, 145-154.

Floryszak-Wieczorek J, Milczarek G, Arasim-owicz M, Ciszewski A. 2006. Do nitric oxide donors mimic endogenous NO-related response in plants? Planta 224, 1363-1372.

Giannopolitis CN, Ries SK. 1977. Superoxide dismutases I. Occurrence in higher plants. Plant Physiology 59, 309-314.

Hao GP, Xing Y, Zhang JH. 2008. Role of Nitric Oxide Dependence on Nitric Oxide Synthase‐like Activity in the Water Stress Signaling of Maize Seedling. Journal of integrative plant biology 50, 435-442.

Heath RL, Packer L. 1968. Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of biochemistry and biophysics 125, 189-198.

Kang HM, Saltveit M. 2002. Effect of chilling on antioxidant enzymes and DPPH‐radical scavenging activity of high‐and low‐vigour cucumber seedling radicles. Plant, Cell & Environment 25, 1233-1238.

Kazemi N, Khavari-Nejad RA, Fahimi H, Saadatmand S, Nejad-Sattari T.2010. Effects of exogenous salicylic acid and nitric oxide on lipid peroxidation and antioxidant enzyme activities in leaves of Brassica napus L. under nickel stress. Scientia Horticulturae 126, 402-407.

Lai T, Wang Y, Li B, Qin G, Tian S. 2011. Defense responses of tomato fruit to exogenous nitric oxide during postharvest storage. Postharvest Biology and Technology 62, 127-132.

Lamattina L, García-Mata C, Graziano M, Pagnussat G. 2003. Nitric oxide: the versatility of an extensive signal molecule. Annual Review of Plant Biology 54, 109-136.

Liu Y, Jiang H, Zhao Z, An L. 2010. Nitric oxide synthase like activity-dependent nitric oxide production protects against chilling-induced oxidative damage in Chorispora bungeana suspension cultured cells. Plant Physiology and Biochemistry 48, 936-944.

Nakano Y, Asada K. 1981. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant and Cell Physiology 22, 867-880.

Neill S, Bright J, Desikan R, Hancock J, Harrison J, Wilson I. 2008. Nitric oxide evolution and perception. Journal of Experimental Botany 59, 25-35.

Pakkish Z, Tabatabaienia MS. 2016. The use and mechanism of NO to prevent frost damage to flower of apricot. Scientia Horticulturae 198, 318-325.

Sala JM. 1998. Involvement of oxidative stress in chilling injury in cold-stored mandarin fruits. Posth-arvest Biology and Technology 13, 255-261.

Schroeter H, Boyd C, Spencer JP, Williams RJ, Cadenas E, Rice-Evans C. 2002. MAPK signaling in neurodegeneration: influences of flavonoids and of nitric oxide. Neurobiology of aging 23, 861-880.

Sevillano L, Sanchez-Ballesta MT, Romojaro F, Flores FB.2009. Physiological, hormonal and molecular mechanisms regulating chilling injury in horticultural species. Postharvest technologies applied to reduce its impact. Journal of the Science of Food and Agriculture 89, 555-573.

Slinkard K, Singleton VL. 1977. Total phenol analysis: automation and comparison with manual methods. American Journal of Enology and Viticu-lture 28, 49-55.

Tajvar Y, Ghazvini RF, Hamidoghli Y, Sajedi RH. 2011. Antioxidant changes of Thomson navel orange (Citrus sinensis) on three rootstocks under low temperature stress. Horticulture, Environment, and Biotechnology 52, 576-580.

Tanou G, Job C, Rajjou L, Arc E, Belghazi M, Diamantidis G, Molassiotis A, Job D. 2009. Proteomics reveals the overlapping roles of hydrogen peroxide and nitric oxide in the acclimation of citrus plants to salinity. The Plant Journal 60, 795-804.

Wills R, Ku V, Leshem Y. 2000. Fumigation with nitric oxide to extend the postharvest life of strawberries. Postharvest Biology and Technology 18, 75-79.

Xu Y, Sun X, Jin J, Zhou H. 2010. Protective effect of nitric oxide on light-induced oxidative damage in leaves of tall fescue. Journal of plant physiology 167, 512-518.

Yang H, Wu F, Cheng J. 2011. Reduced chilling injury in cucumber by nitric oxide and the antioxidant response. Food chemistry 127, 1237-1242. 

Yelenosky G. 1985. Cold hardiness in citrus. Horticultural Reviews, Volume 7, 201-238.

Zhang Z, Wang H, Wang X, Bi Y. 2011. Nitric oxide enhances aluminum tolerance by affecting cell wall polysaccharides in rice roots. Plant cell reports 30, 1701-1711.

Zhao M-G, Chen L, Zhang L-L, Zhang W-H. 2009. Nitric reductase-dependent nitric oxide production is involved in cold acclimation and freezing tolerance in Arabidopsis. Plant Physiology 151, 755-767.

Zheng Y, Fung RW, Wang SY, Wang CY. 2008. Transcript levels of antioxidative genes and oxygen radical scavenging enzyme activities in chilled zucchini squash in response to superatmospheric oxygen. Postharvest Biology and Technology 47, 151-158.

Zhu S, Sun L, Liu M, Zhou J. 2008. Effect of nitric oxide on reactive oxygen species and antioxidant enzymes in kiwifruit during storage. Journal of the Science of Food and Agriculture 88, 2324-2331.