Novel approach towards advanced tools in biosciences and their applications

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Research Paper 01/08/2020
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Novel approach towards advanced tools in biosciences and their applications

Faisal Mehmood, Nelofar Ansari, Muhammad Shakir, Qasim Farooq, Tooba Zafar, Kinza Shahid, Haiqa Masoud, Qurat ul Ain, Sania Riasat
J. Bio. Env. Sci.17( 2), 15-21, August 2020.
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Recombinant vaccines have been used for the treatment of various diseases and important advancements have been made in the field of health sciences for prevention of infectious diseases. Recombinant vaccines are those vaccines that rely on the principle of making only a small fragment of antigen responsible for triggering antibodies response. Bacterial expression systems are widely used for their ease of production and higher levels of expression of these vaccines. For vaccines that require post-translational modification we use eukaryotic cells like yeast or mammalian cells. Most of the vaccines that are being synthesized today in recombinant DNA technology are highly purified recombinant proteins. CRISPR is underway to eliminate or rectify the gene causing the cancerous mutation. Microarray is helpful in the identification of differentiation of gene expression after exposure to a toxic chemical to identify which of the tissues or proteins are being affected by that specific toxin or chemical. The microorganisms that live in our guts have been found to legitimately impact numerous aspects of our physiology such as probiotics that containing bacteria used for digestive system. Improvement of engineered pathways and creatures to blend biofuels and medications will proceed.


Abberton M, Batley J, Bentley A. 2016. Global agricultural intensification during climate change: a role for genomics. Plant biotechnology journal 14, 1095-1098.

Adomas A, Heller G, Olson A, Osborne J, Karlsson M, Nahalkova J, Van Zyl L, Sederoff R, Stenlid J, Finlay R, Asiegbu FO. 2008. Amp;quot;Comparative analysis of transcript abundance in Pinus Sylvester’s after challenge with a saprotrophic, pathogenic or mutualistic fungus &quot. Tree Physiol 28(6), 885–897.

Baker M. 2011. Synthetic genomes: The next step for the synthetic genome & amp; quot. Nature 473(7347), 403

Bardhan IR, Thouin MF. 2013. Health information technology and its impact on the quality and cost of healthcare delivery. Decision Support Systems 55, 438-449.

Brar SK, Dhillon GS, Soccol CR, 2013. Biotransformation of waste biomass into high value biochemicals, Springer.

Carter S, Costa PF, Vaquette C, Ivanovski S, Hutmacher DW, Malda J. 2017. Additive biomanufacturing: an advanced approach for periodontal tissue regeneration. Annals of biomedical engineering 45, 12-22.

Cai L, Fisher AL, Huang H, Xie Z. 2016. CRISPR-mediated genome editing and human diseases & amp quot. Genes and amp;amp. Diseases 3(4), 244-251.

Gleba Y, Klimyuk V, Marillonnet S. 2005. Magnifection—a new platform for expressing recombinant vaccines in plants. Vaccine 23, 2042-2048.

Govan V. 2008. A novel vaccine for cervical cancer: quadrivalent human papillomavirus (types 6, 11, 16 and 18) recombinant vaccine (Gardasil). Ther Clin Risk Manag 4, 65-70.

Hendel A, Bak RO, Clark JT, Kennedy AB, Ryan DE, Roy S, Steinfeld I, Lunstad BD, Kaiser RJ, Wilkens AB, Bacchetta R, Tsalenko A, Dellinger D, Bruhn L, Porteus MH. 2015. Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells. Nature Biotechnology 33(9), 985-9.

Levsky JM, Singer RH. 2003. Fluorescence in situ hybridization: past, present and future. Journal of cell science 116, 2833-2838.

Petricoin Iii, Hackett JL, Lesko LJ, Puri RK, Gutman SI, Chumakov K, Woodcock J, Feigal Jr, Zoon KC, Sistare FD. 2002. Medical applications of microarray technologies: a regulatory science perspective. Nature genetics 32, 474.

Silva NFD, Passos NS. 2002. DNA forense: coleta de amostras biológicas em locais de crime para estudo do DNA. Maceió: Ed UFAL.

Sturn A, Quackenbush J, Trajanoski Z. 2002. Genesis: cluster analysis of microarray data. Bioinformatics 18, 207-208.

Zand M, Narasu M. 2013. A review article Biotechnology Applications in Medicine. Intl. Res. J. Appl. Basic. Sci 4, 2557-2563.

Zhang Y, Sun J. 2017. Biomanufacturing: history and perspective. Journal of industrial microbiology & biotechnology 44, 773-784.