NS3-4A and NS4A proteins of hepatitis C virus regulate the apoptotic proteins expression in Huh-7 cells

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NS3-4A and NS4A proteins of hepatitis C virus regulate the apoptotic proteins expression in Huh-7 cells

Farakh Javed, Sobia Manzoor, Aamer Ali Khattak, Naureen Aurangzeb, Sadiq Noor Khan, Darima Habib, Afshan Saleem
Int. J. Biosci.7( 5), 9-15, November 2015.
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Almost 2 % of the world population is infected with Hepatitis C virus (HCV). Prolonged HCV infection often results in the development of chronic hepatitis, liver cirrhosis and hepatocellular carcinoma (HCC) that is severe health problem worldwide. Aim of the current study was to check the role of HCV non-structural NS3-4A and NS4A proteins in the regulation of mitochondrial death pathway. Recombinant vectors containing HCV non-structural NS3-4A and NS4A proteins were transfected in Huh-7 cells and their transfection efficacy were observed. Further confocal microscopy was used to observe the accumulation of HCV the non-structural proteins NS3-4A and NS4A to mitochondria. Western blot analysis shown down regulated expression of Bcl-2protein which is anti-apoptotic in nature in the HCV non-structural NS3-4A and NS4A expressing Huh-7 cells. Bad protein which is basically pro-apoptotic in nature shown up regulated expression in NS3-4A and NS4A expressing Huh-7 cells. Furthermore HCV non-structural NS3-4A and NS4A proteins expressing Huh-7 cells have shown the activation of executioner procaspase-3 which is the final pro-apoptotic mediator that cleavage the numerous structural and regulatory cellular proteins. In conclusion HCV non-structural NS3-4A and NS4A proteins have shown regulation of apoptotic proteins that are clear indicator of the induction of mitochondrial death pathway.


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