Welcome to International Network for Natural Sciences | INNSpub

Ontogeny of embryogenic aggregates in suspension culture of diploid watermelon [Citrullus lanatus (Thunb.)]

Research Paper | January 1, 2012

| Download 5

Rubaiyat Sharmin Sultana, Md. Mahabubur Rahman

Key Words:

Int. J. Agron. Agri. Res.2( 1), 40-46, January 2012


IJAAR 2012 [Generate Certificate]


Cell suspension culture from leaf-derived callus of diploid watermelon [Citrullus lanatus (Thunb.)] was established. The callus obtained on agar-gelled Murashige and Skoog (MS) medium containing 2.5 mgl-1 2,4-dichlorophenoxyacetic acid (2,4-D) was used for suspension culture. The growth of cell in the suspension culture was the highest in liquid MS medium containing same concentration (2.5 mgl-1) of 2,4-D as used for callus induction. Cells in suspension culture underwent division as result both free cells and cell aggregates were formed. In first 15 days, profuse number of early proembryogenic structures (two-, three- and four-celled) was observed while after more 15 days of culture, multi-celled proembryogenic structure was greater in suspension. The initial amount of cell in suspension culture was effective for cell proliferation and cell aggregation. As initial amount of cells, 4 ml sedimented cell volumes (SCV) could proliferate and aggregate at a highest level after 30 days of culture. Three typical phages (lag, exponential and stationary phases) in S-shaped growth curve were found in the batch culture. Among the cell growth phages, duration of exponential phage is important for the high production of cell and biosynthesis of cell compounds.


Copyright © 2012
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Ontogeny of embryogenic aggregates in suspension culture of diploid watermelon [Citrullus lanatus (Thunb.)]

Adelberg J, Rhodes BB, Skorupska H. 1990. Generating tetraploid melons from tissue culture. Horticultural Science 25, 73.

Anbazhagan VR, Ganapathi A. 1999. Somatic embryogenesis in cell suspension cultures of pigeonpea (Cajanus cajan L.). Plant Cell, Tissue and Organ Culture 56, 179–184.

Bhojwani SS, Razdan MK. 1996. Cell culture. In: S.S. Bhojwani, M.K. Razdan Eds. Plant tissue culture: Theory and practice. A revised edition. Elsevier, Amsterdam, 63-93.

Blackmon WJ, Reynolds BD. 1982. In vitro shoot regeneration of Hibiscus acetosella, muskmelon, watermelon and winged bean. Horticultural Science 17, 558-589.

Choi PS, Soh WY, Kim YS, Yoo OJ, Liu JR. 1994. Genetic transformation and plant regeneration of watermelon using Agrobacterium tumefaciens. Plant Cell Reports 13, 344-348.

Compton ME, Gray DJ, Elmstrom GW. 1994a. The identification of tetraploid regenerants from cotyledons of diploid watermelon and their use in breeding triploid hybrids. Horticultural Science 29, 450.

Compton ME, Gray DJ, Maynard DN. 1996b. Use of tetraploid somaclones in breeding seedless watermelons. In Vitro 32, 28A.

Compton ME, Gray DJ. 1991. Shoot organogenesis on cotyledons of watermelon. Horticultural Science 26, 772.

Compton ME, Gray DJ. 1993b. Somatic embryogenesis and plant regeneration from immature cotyledons of watermelon. Plant Cell Reports 12, 61-65.

Compton ME, Gray DJ, Elmstrom GW. 1996a. Identification of tetraploid regenerants from cotyledons of diploid watermelon cultured in vitro. Euphytica 87,165-172.

Compton ME, Gray DJ, Hiebert E, Lin CM. 1994b. Microprojectile bombardment prior to co-cultivation with Agrobacterium improves GUS expression in watermelon cotyledons. In Vitro 30A, 62.

Compton ME, Gray DJ. 1993a. Shoot organogenesis and plant regeneration from cotyledons of diploid, triploid and tetraploid watermelon. Journal of The American Society for Horticaltural Science 118, 151-157.

Compton ME, Gray DJ. 1994. Adventitious shoot organogenesis and plant regeneration from cotyledons of tetraploid watermelon. Horticultural Science 29, 211-213.

Compton ME. 1997. Influence of seedling pretreatment and explant type on watermelon shoot organogenesis. Horticultural Science 32, 514.

Dong JZ, Jia SR. 1991. High efficiency plant regeneration from cotyledons of watermelon (Citrullus vulgaris Schrad.). Plant Cell Reports 9, 559-562.

Eapen S, George L. 1993. Somatic embryogenesis in peanut: influence of growth regulators and sugars. Plant Cell, Tissue and Organ Culture 35, 151–156.

Hao LX, Wang HM. 1998. A study on building up the regeneration system of watermelon. Acta Agriculturae-Boreali-Sinica 13,112-115.

Jaworski JM, Compton ME. 1997. Plant regeneration from cotyledons of five watermelon cultivars. Horticultural Science 32, 469-470.

Krogstrup P. 1990. Effect of culture densities on cell proliferation and regeneration from embryogenic cell suspensions of Picea sitchensis. Plant Science 72, 115-123.

Mohamed SV, Wang CS, Thiruvengadam M, Jayabalan N. 2004. In vitro plant regeneration via. somatic embryogenesis through cell suspension cultures of horsegram [Macrotyloma uniflorum (Lam.) Verdc.]. In Vitro Cellular & Developmental Biology-Plant 40, 284–289.

Murashige T, Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiology, 15, 473-497.

Ogita S, Sasamoto H, Kubo T. 2000. Control of the development of somatic embryo of Japanese conifers by the density of embryogenic cells in liquid culture. In: C. Kubota, C. Chun eds. Transplant production in The 21st Century. Kluwer Academic Publishers, The Netherlands, 209-214.

Park RHR, Russell RI. 1991. Watermelon stomach. British Journal of Surgery 78, 395-396.

Razdan MK. 1993. Cell culture: An Introduction to Plant Tissue Culture. Oxfort and IBH Publishing Co. PVT. Ltd. New Delhi, India.

Srivastava DR, Andrianov VM, Piruzian ES. 1989. Tissue culture and plant regeneration of watermelon (Citrullus vulgaris Schrad. cv. Melitopolski). Plant Cell Reports 8, 300-302.

Sultana RS, Bari MA, Rahman MH, RAhman MM, Siddique NA, Khatun N. 2004. In vitro regeneration of plantlets from leaf explant of watermelon (Citrullus lanatus Thunb.) Biotechnology 3, 131-135.

Sultana RS, Rahman MM. 2011. Cell proliferation and cell aggregate development in suspension culture of sweet potato (Ipomoea batatas L.). International Journal of Biosciences 1, 6-13.

Tabei Y. 1997. Study on breeding of cucurbitaceae using biotechnology. Bull. Natl Inst. Agrobiol. 11,1-107.

Thomas E, Street HE. 1969. Organogenesis in Cell Suspension Cultures of Atropa belladonna L. and Atropa belladonna Cultivar lutea Döll. Annals of Botany 34 (3), 657-669.