Optimization of β-galactosidase production by response surface methodology

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Research Paper 01/12/2011
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Optimization of β-galactosidase production by response surface methodology

Raghunath Rashmi, Kora Rudraiah Siddalingamurthy
Int. J. Biosci.1( 6), 119-127, December 2011.
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β-galactosidase production is carried out using lactose or lactose rich substrates like whey. Tamarind seed powder has not been reported as a substrate for production of this enzyme before. In this study, statistical optimization of medium components for production of β-galactosidase, using tamarind seed powder, by Aspergillus terreus was attempted. Screening for the effects of eleven medium components on enzyme activity was carried out by Plackett-Burman design which showed that NH4(SO4)2, lactose and MgSO4 has significant (p<0.001) positive influence and pH, yeast extract, maltose and NaNO3 has significant negative influence. Optimal levels of positively influencing parameters were determined by ridge analysis and was found to be 2.97, 2.88 and 2.67 g/L of NH4(SO4)2, lactose and MgSO4 respectively. In the optimized medium, enzyme activity increased 2.8 folds in comparison with basal medium. Improved activity, being achieved by the use of a cheaper substrate, could reduce the cost of production of the enzyme.


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