Optimization of an efficient system for the In Vitro Organogenesis of Sugarcane (Saccharum Officinarum L.) from apical shoot explant using different plant growth regulators
Paper Details
Optimization of an efficient system for the In Vitro Organogenesis of Sugarcane (Saccharum Officinarum L.) from apical shoot explant using different plant growth regulators
Abstract
An efficient in vitro system provides considerable opportunities to regenerable target tissues in plants. The large scale multiplication of newly released sugarcane varieties still remains inexplicit. Here, we investigate the response of three different sugarcane varieties (CP 77400, CP2086, and M 93) towards the onset of callus induction and adventitious organogenesis. Young leaves of apical shoot were subjected to Murashige and Skoog (MS) medium with various combination and concentrations of growth regulators for callus induction, adventitious shoot morphogenesis and rooting. Our results indicated that highest percent callus induction was observed in variety CP77400 which was 80% when explants were cultured on MS medium supplemented with 3.5 mg/l auxin after 14 days of incubation. The maximum adventitious shoot induction was observed in CP 77400 variety incubated with MS medium supplemented with 2.5 mg/l auxin and 1 mg/l cytokinin. Morover, sugarcane variety CP 77400 showed highest rate of in vitro root induction when incubated with half MS basal medium supplemented 3 mg/l auxin alone. Regarding the mean number of roots per shoots and the average length of the roots, the variety CP77400 showed the highest number of roots per shoots (8.4) and the highest root length (6.5 cm) on half-strength MS medium supplemented with auxin (3 mg/L). Thus, these findings can be used to promote mass production of different varieties of sugarcane which may overcome the present trend of demand future.
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