Optimization of an efficient system for the In Vitro Organogenesis of Sugarcane (Saccharum Officinarum L.) from apical shoot explant using different plant growth regulators

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Research Paper 01/12/2019
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Optimization of an efficient system for the In Vitro Organogenesis of Sugarcane (Saccharum Officinarum L.) from apical shoot explant using different plant growth regulators

Naveed Ahmad, Muhammad Ishaq, Ajmal Mandozai, Asif Ali Khan, Noor al amin, Muhammad Inam, Raza Ullah, Hina Shaukat, Muhammad Abrar, Ismail Khan, Raham Sher Khan
Int. J. Biosci.15( 6), 63-72, December 2019.
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Abstract

An efficient in vitro system provides considerable opportunities to regenerable target tissues in plants. The large scale multiplication of newly released sugarcane varieties still remains inexplicit. Here, we investigate the response of three different sugarcane varieties (CP 77400, CP2086, and M 93) towards the onset of callus induction and adventitious organogenesis. Young leaves of apical shoot were subjected to Murashige and Skoog (MS) medium with various combination and concentrations of growth regulators for callus induction, adventitious shoot morphogenesis and rooting. Our results indicated that highest percent callus induction was observed in variety CP77400 which was 80% when explants were cultured on MS medium supplemented with 3.5 mg/l auxin after 14 days of incubation. The maximum adventitious shoot induction was observed in CP 77400 variety incubated with MS medium supplemented with 2.5 mg/l auxin and 1 mg/l cytokinin. Morover, sugarcane variety CP 77400 showed highest rate of in vitro root induction when incubated with half MS basal medium supplemented 3 mg/l auxin alone. Regarding the mean number of roots per shoots and the average length of the roots, the variety CP77400 showed the highest number of roots per shoots (8.4) and the highest root length (6.5 cm) on half-strength MS medium supplemented with auxin (3 mg/L). Thus, these findings can be used to promote mass production of different varieties of sugarcane which may overcome the present trend of demand future.

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Ahmad N, Jianyu L, Xu T, Noman M, Jameel, A, Na Y, Yuanyuan D, Nan W, Xiaowei L, Fawei W, Xiuming L, Haiyan L. 2019. Overexpression of a Novel Cytochrome P450 Promotes Flavonoid Biosynthesis and Osmotic Stress Tolerance in Transgenic Arabidopsis. Genes 10, 756. http://dx.doi.org/10.3390/genes10100756

Ali A, Naz S, Siddiqui FA, Iqbal J. 2008. An efficient protocol for large scale production of sugarcane through micropropagation. Pakistan Journal of Botany 40(1), 139-149.

Baksha R, Alam R, Karim MZ, Paul SK, Hossain MA, Miah MAS, Rahman ABMM. 2002. In vitro shoot tip culture of sugarcane (Saccharum officinarum) variety LSD28. Biotechnology 1(2-4), 67-72.

Begum S, Hakim L, Azam MA. 1995. Efficient regeneration of plants from leaf base callus in sugarcane. Plant Tissue Culture 5, 1-5.

Brisibe EA, Miyake H, Taniguchi T, Maeda E. 1994. Regulation of somatic embryogenesis in long term callus cultures of sugarcane (Saccharum officinarum L.). New Phytologist 126, 301-307.  https://doi.org/10.1111/j.1469-8137.1994.tb03949.x

Geetha S, Padmanadhan D. 2001. Effect of hormones on direct somatic embryogenesis in sugarcane. Sugar Technology 3, 120-121. https://doi.org/10.1007/BF03014576

Gosal SS, Thind KL, Dhaliwal HS. 1998. Micropropagation of sugarcane. An efficient protocol for commercial plant production. Crop Improvement. 2, 167-171.

Islam AS, Begum HA, Haque MM. 1982. Regeneration of Saccharum officinarum for disease resistant varieties. Proceedings International Congress of Plant Tissue and Cell Culture 5, 709-710.

Kerstetter RA, Hake S. 1997. Shoot meristem formation in vegetative development. Plant Cell. 9, 1001-1010. https://doi.org/10.1105/tpc.9.7.1001

Khan IA, Dahot M, Yasmin S, Khatri A, Seema N, Naqvi MH. 2006. Effect of sucrose and growth regulators on the micropropagation of Sugarcane clones. Pakistan Journal of Botany 38(4), 961-967.

Khan SA, Rashid H, Chaudhary MF, Chaudary Z, Afroz A. 2008. Rapid Micropropogation of three elite sugarcane varities by shoot tip culture. African Journal of Biotechnology 7, 2174-2180.

Khan SA, Rashid H, Chaudhary MF, Chaudhary Z, Fatima Z, Siddiqui SU, Zia M. 2009. Effect of cytokinins on shoot multiplication in three elite sugarcane varieties. Pakistan Journal of Botany 41(4), 1651-1658.

Khatri A, Khan IA, Javed MA, Siddiqui MA, Khan MKR, Khanzada MH, Dahar NA, Khan R. 2002. Studies on callusing and regeneration potential of indigenous and exotic sugarcane clones. Asian Journal of Plant Sciences 1(1), 41-43. http://dx.doi.org/10.3923/ajps.2002.41.43

Nand L, Singh HN. 1994. Rapid clonal multiplication of sugarcane through tissue culture. Plant Tissue culture 4, 1-7.

Nonato RV, Mantelatto PE, Rossell CEV. 2001. Integrated production of biodegradable plastic, sugar and ethanol. Applied Microbiology and Biotechnology 57, 1-5. https://doi.org/10.1007/s002530100732

Parmessur Y, Aljanabi A, Saumtally S,   Dookun-Saumtally A. 2002. Sugarcane yellow leaf virus and sugarcane yellows phytoplasma: elimination by tissue culture. Plant Pathology 51, 561-566. https://doi.org/10.1046/j.1365-3059.2002.00747.x

Raza G, Ali K, Muhkhtiar Z, Mansoor M, Arshad M, Asad S. 2010. The response of sugarcane (Saccharum officinarum L.) genotypes to callus induction, regeneration and different concentrations of the selective agent (geneticin-418). African Journal of Biotechnology 9(51), 8738-8747.

Rugini E, Cristofori V, Silvestri C. 2016. Genetic improvement of olive (olea europaea L.) by conventional and in vitro biotechnology methods. Biotechnology Advances 34(5), 687-696. http://dx.doi.org/10.1016/j.biotechadv.2016.03.004

Silvestri C, Cristofori V, Ceccarelli M, Caceres, ME, Escribà -Lacuesta J, Rugini E. 2016. Adventitious shoot organogenesis from leaf and petiole explants of european hazelnut. Plant Cell, Tissue and Organ Culture 126(1), 59-65. http://dx.doi.org/10.1007/s11240-016-0976-7

Tarique HM, Mannan MA, Bhuiyan MSR, Rahaman MM. 2010. Micropropagation of sugarcane through leaf sheath culture. International Journal of Sustainable Crop Production 2(2), 13-15.

Tiwari AK, Bharti YP, Tripathi S, Mishra N, Lal M. 2010. Biotechnological Approaches to Improve Sugarcane Crop with Special Reference to Disease Resistance. Acta Phytopathologica et Entomologica Hungarica 45, 235-249. http://dx.doi.org/10.1556/APhyt.45.2010.2.1

Yadav S, Ahmad A, Rastogi J, Lal M. 2012. Tissue culture strategies in sugarcane (Saccharum officinarum L.). International Journal of Pharma and Bio Sciences 3(2), 427-441.