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Research Paper | January 1, 2018

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Optimization of laboratory requirements through experimental design for maximum growth of indigenous saccharomyces cerevisiae using apple waste as substrate

Faheem Ahmed Khan, Sarzamin Khan, Nafees Bacha, Tariq Khan

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Int. J. Biosci.12(1), 136-142, January 2018

DOI: http://dx.doi.org/10.12692/ijb/12.1.136-142


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From ancient wild type Saccharomyces Cerevisiae is used for production of valuable products. These microorganisms can be grown on a number of carbohydrate rich waste materials. For optimum valuable products It is needed to optimize different parameters for growth of indigenous S. cerevisiae utilizing apple waste. For the said purpose Indigenous S. cerevisiae was isolated from different fruit samples and identified by Polymerase chain reaction (PCR). Apple waste was collected and chemically treated to convert complex polysaccharide into simple one. For optimum growth, different laboratory parameters i.e.pH, temperature, shaking and glucose concentration were optimized using response surface methodology. Dry microbial biomass was analyzed for proximate composition i.e. crude protein, crude fibers, crude fats, total carbohydrates and ash contents. Dry microbial biomass was also evaluated for the presence of different amino acids through aminoacid analyzer using orthophthalaldehyde (OPA) as a fluorescent agent. Results revealed that 2.7% glucose, 32oC temperature, pH 5 and shaking at 150 rpm were best for optimum growth of indigenous S. cerevisiae. Dry microbial biomass was rich in crude proteins (44.65%) followed by carbohydrate (43.09%). It was observed that dry microbial biomass was rich in aspartic acid and leucine (14.57%) each, followed by serine (12.89%) and alanine (11.37%).From the present study it is concluded that using response surface methodology different growth parameters can be optimized for indigenous S. cerevisiae on apple waste. Dry microbial biomass is rich in crude protein and essential amino acids therefore it can be used as a source of single cell protein.


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Optimization of laboratory requirements through experimental design for maximum growth of indigenous saccharomyces cerevisiae using apple waste as substrate

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