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Research Paper | July 1, 2017

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Optimization of nutritional conditions and effect of time and temperature for the production of bacterial biocontrol agents

Shazia Shahzaman, Riaz Ali Gardazi, Muhammad Inam-ul-Haq, Asif Hussain Mastoi, Sikander Hayat, Raees Ahmed

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Int. J. Biosci.11(1), 141-146, July 2017

DOI: http://dx.doi.org/10.12692/ijb/11.1.141-146

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Abstract

Yield potential is drastically affected by a number of soil borne fungal pathogens which result into quality and quantity reduction. Plant growth promoting rhizobacteria (PGPR) colonizing the rhizosphere result into disease control and also enhance the plant growth characters. The population dynamics of the bio-agents in prepared formulations viz., Pseudomonas fluorescens on vermicompost (PFV), Pseudomonas fluorescens on Organic matter (PFOM), Bacillus subtilis on Vermi compost (BSV) and Bacillus subtilis on Organic matter (BSOM) was determined at 7, 30, 60, 90 and 120 days after storage (DAS) at 5 ºC and 28 ºC. An increasing trend in colony forming unit was seen with the increase in time interval and maximum cfu (312.67 cfu) was recorded at 60 DAS in the treatments stored at 28 ºC compared with (193.67 cfu) at 5 ºC. Decrease in population was observed at 90 DAS and lowest bacterial population was recorded at 120 DAS at both 5 ºC and 28 ºC.

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Optimization of nutritional conditions and effect of time and temperature for the production of bacterial biocontrol agents

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