Optimization of nutritional conditions and effect of time and temperature for the production of bacterial biocontrol agents

Paper Details

Research Paper 01/07/2017
Views (342) Download (12)

Optimization of nutritional conditions and effect of time and temperature for the production of bacterial biocontrol agents

Shazia Shahzaman, Riaz Ali Gardazi, Muhammad Inam-ul-Haq, Asif Hussain Mastoi, Sikander Hayat, Raees Ahmed
Int. J. Biosci.11( 1), 141-146, July 2017.
Certificate: IJB 2017 [Generate Certificate]


Yield potential is drastically affected by a number of soil borne fungal pathogens which result into quality and quantity reduction. Plant growth promoting rhizobacteria (PGPR) colonizing the rhizosphere result into disease control and also enhance the plant growth characters. The population dynamics of the bio-agents in prepared formulations viz., Pseudomonas fluorescens on vermicompost (PFV), Pseudomonas fluorescens on Organic matter (PFOM), Bacillus subtilis on Vermi compost (BSV) and Bacillus subtilis on Organic matter (BSOM) was determined at 7, 30, 60, 90 and 120 days after storage (DAS) at 5 ºC and 28 ºC. An increasing trend in colony forming unit was seen with the increase in time interval and maximum cfu (312.67 cfu) was recorded at 60 DAS in the treatments stored at 28 ºC compared with (193.67 cfu) at 5 ºC. Decrease in population was observed at 90 DAS and lowest bacterial population was recorded at 120 DAS at both 5 ºC and 28 ºC.


Bazilah A, Sariah M, Abidin MZ, Yasmeen S. 2011. Effect of carrier and temperature on the viability of Burkholderia sp. (UPMB3) and Pseudomonas sp. (UPMP3) during storage. International Journal of Agriculture and Biology 13, 198-202.

Garcia de Salamone IE. 2000. Direct beneficial effects of cytokinin-producing rhizobacteria on plant growth.

Goldman L. 2008. Encyclopedia of public health: fungicides. Cited on 6 June 2008.

Kloepper J, Schroth M. 1981. Development of a powder formulation of rhizobacteria for inoculation of potato seed pieces. Phytopathology 71(6), 590- 592.

Lugtenberg B, Kamilova F. 2009. Plant-growth-promoting rhizobacteria. Annual review of Microbiology 63, 541-556.

Maheshwari D, Dubey R, Aeron A, Kumar B, Kumar S, Tewari S, Arora NK. 2012. Integrated approach for disease management and growth enhancement of Sesamum indicum L. utilizing Azotobacter chroococcum TRA2 and chemical fertilizer. World Journal of Microbiology and Biotechnology 28(10), 3015-3024.

Ndoumbe-Nkeng M, Sache I. 2003. Lutte contre la pourriture brune des cabosses du cacaoyer au Cameroun: Améliorer les connaissances épidémiologiques afin d’intervenir au bon moment. Phytoma-La Défense des Végétaux (562), 10-12.

Nga NTT, Giau N, Long N, Lübeck M, Shetty NP, De Neergaard E, Thuy TTT, Kim P, Jørgensen HJL. 2010. Rhizobacterially induced protection of watermelon against Didymella bryoniae. Journal of applied microbiology 109(2), 567-582.

Omer AM. 2010. Bioformulations of Bacillus spores for using as biofertilizer. Life Sciences Journal 7, 124-131.

Sahni S, Sarma B, Singh D, Singh H, Singh K. 2008. Vermicompost enhances performance of plant growth-promoting rhizobacteria in Cicer arietinum rhizosphere against Sclerotium rolfsii. Crop Protection 27(3), 369-376.

Sanco D. 2014. EU pesticides database. MRLs updated on 28(01), 2014.

Vessey JK. 2003. Plant growth promoting rhizobacteria as biofertilizers. Plant and soil 255(2), 571-586.

Woltz S, Jones J. 1981. Nutritional requirements of Fusarium oxysporum: Basis for a disease control system. In ‘Fusarium: diseases, biology and taxonomy’. (Eds PE Nelson, TA Toussoun, RJ Cook) pp. 340–349. The Pennysylvania State University Press: University Park.