Optimization, purification and characterization of invertase by Pseudomonas sp, isolated from the cane molasse
Paper Details
Optimization, purification and characterization of invertase by Pseudomonas sp, isolated from the cane molasse
Abstract
A Pseudomonas sp was isolated from cane molasse sample. The effect of different production parameters such as pH, temperature, incubation time, carbon source, nitrogen source (organic and inorganic), on invertase production by the isolated Pseudomonas sp strain were studied. The enzyme production was assayed in submerged fermentation (SmF). Maximum invertase activity was found at pH 6, 40°C, 48 hours, sucrose, yeast extract. The enzyme was purified by ammonium sulfate precipitation, dialysis and DEAE cellulose column chromatography. A trial for the purification of invertase resulted in an enzyme with specific activity of 39.56, with 25.7% recovery of invertase. More over this study gives us a hint of microbial wealth of invertase production which can be harnessed for biotechnological processes.
Chen JS, Saxton J, Hemming FW, Peberdy JF. 1995.Purification and partial characterization of the high and low molecular weight form (Sand F-form) of invertase secreted by Aspergillus nidulans. Biochim. Biophys. Acta. 1296(2), 207-18.
Gascón S, Neumann NP, Lampen JO. 1968. Comparative study of the properties of the purified internal and external invertases from yeast. J. Biological. Chemistry 243, 1573-1577.
Gehlawae J. 2001. New technology for invert sugar and high fructose syrups from sugar cane. Indian Journal of Chemical Technology 8, 28-32
Hussain A, Rashid MH, Perveen R. 2009. Ashraf M. Purification, kinetic and thermodynamic characterization of soluble acid invertase from sugarcane (Saccharum officinarum L.). Plant Physiol Biochem 47,188-194.
Kaur N Sharma AD. 2005. Production, optimization and characterization of extracellular invertase by an actinomycete strain. Journal of Scientific Industrial Research 64, 515-519.
Kim BW, Kwon HJ, Park HY, Nam SW, Park JP, Yan JW.2000. Production of novel transfructosylating enzyme from Bacillus macerans EG-6, Bioprocess Engineering 23, 11-16.
Kumas R, Kesavapillai B. 2012. Stimilation of extracellular invertase production from spent yeast when sugarcane pressmud used as substrate through solid state fermentation. J of Microbiology and Bioyechnology 1(1), 81.
Mahendran S, Sankaralingam S, Shankar T, Vijayabaskar P. 2010. Alkalophilic protease enzyme production from estuarine Bacillus aquimaris, World Journal of Fish and Marine Science 2(5), 436-443.
Mona MR, Mohamed UN. 2009. Production, Purification and Characterization of Extracellular Invertase from Saccharomyces Cerevisiae NRRL Y12632 by Solid-State Fermentation of Red Carrot Residue. Aust. J. Basic Applied. Science 3(3), 1910-1919.
Nelson N. 1944. Aphotometric adaptation of somogyi method for the determination of glucose, J Biological Chemistry 153, 315-380.
Oda Y. and Tonomura K. 1994. Purification and characterization of invertase from torulaspora pretoriensis YK1. Bioscience Biotechnol Biochem. 58,1155-1157.
Park YK, Sato H.1982. Fungal invertase as an aid for fermentation of cane molasses into ethanol, Appl. and Envirron. Microbiol 44(4), 988-989.
Rouwenhorst RJ, van der Baan AA, Scheffers WA. and Van Dijken JP. 1991. Production and localization of beta-fructosidase in asynchronous and synchronous chemostat cultures of yeasts. Appl. Environ. Microbiol. 57(2), 557-562.
Sayago JE, Vattuone MA, Sampietro AR. Isla MI. 2002. Proteinaceous inhibitor versus fructose as modulators of Pteris deflexa invertase activity. J. Enzyme. Inhib. Med. Chem 17(2), 123-130.
Shafiq K, Ali S, Ul-Haq I. 2004. Temperature optima for invertase secretion by yeast in synthetic medium, Pak. J. Life Soc Science 2(1), 21-23.
Su YC, Sheu CS, Chien JY and Tzan TK. 1991. Production of beta-fructofuranosidase with transfruc-tosylating a ctivity for fructooligosaccharides synthesis by Aspergillusjaponicus NTU-1249. Proc. Natl. Sci. Counc. Repub. China 15(3), 131-139.
Uma C, Gomathi D, Muthulakshmi C. Gopalakrishnan V K .2010. Production, Purification and Characterization of Invertase by Aspergillus flavus Using Fruit Peel Waste as Substrate. Advances in Biological Research 4(1), 31-36.
Zouaoui Benattouche, Ghalem Bachir Raho, Fatima Sahnouni, Djillali Bouhadi, 2016. Optimization, purification and characterization of invertase by Pseudomonas sp, isolated from the cane molasse. Int. J. Biosci., 9(1), 349-356.
Copyright © 2016 by the Authors. This article is an open access article and distributed under the terms and conditions of the Creative Commons Attribution 4.0 (CC BY 4.0) license.