Optimizing The Bacteriocin Production In Strain Of Lactobacillus pentosus Isolated From Cheese

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Research Paper 01/12/2020
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Optimizing The Bacteriocin Production In Strain Of Lactobacillus pentosus Isolated From Cheese

Samreen Akhtar, Syed Kashif Nawaz, Faiza Zubair
Int. J. Biosci.17( 6), 507-518, December 2020.
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MRS agar was used for the isolation of Lactobacillus strain. This strain was recognized through biochemical tests and 16S rRNA ribotyping, as Lactobacillus pentosus. Its antibacterial effects were detected by utilizing cell free supernatant (CFS). Escherichia coli, Bacillus subtilis and Staphylococcus aureus were used as test strains. CFS showed antimicrobial activity against the test strains. CFS was treated with proteinases for the confirmation of loss of antimicrobial activity. Loss of antimicrobial activity on exposure to proteinases indicated the presence of bacteriocin in CFS. CFS was also studied for its antimicrobial effect at different temperatures and pH. Optimum antimicrobial effect was recorded at pH 7 and at temperature 45°C. The current study indicates the antimicrobial activity of strain of L. pentosus against E. coli, B. subtilis and S. aureus.


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