Welcome to International Network for Natural Sciences | INNSpub

Optimizing zinc seed priming treatments for improving the germination and early seedling growth of wheat

Research Paper | June 1, 2018

| Download 97

Nazia Hassan, Munazza Yousra, Sair Sarwar, Mohsin Zafar, Muhammad Zameer Khan

Key Words:

Int. J. Agron. Agri. Res.12( 6), 20-29, June 2018


IJAAR 2018 [Generate Certificate]


Zinc (Zn) is an essential micronutrient with various vital metabolic, enzymatic and defensive roles in crop plants. This study was conducted to optimize the seed priming treatments, with Zn in improving the germination and early seedling growth of wheat. Experiments were conducted in petri plates and sand filled pots, respectively in Allelopathy laboratory, Department of Agronomy, University of Agriculture Faisalabad, during 2012. The experiments were laid out in completely randomized design in factorial arrangement with four replications. Seeds of two wheat cultivars Lasani-2008 and Faisalabad-2008 were soaked in aerated Zn solution of various concentrations (0.5, 0.1, 0.05, 0.01, 0.005 and  0.001 M Zn) for 12 h. Seeds soaked in aerated water for 12 h (hydropriming) and untreated dry seeds were taken as control. Wheat seeds primed in 0.1 to 0.01 M Zn solution increased the earliness, uniformity and final germination percentage in wheat. Beyond this concentration, there was adverse effect on germination and seedling growth of both wheat cultivars. Seed priming with 0.1 to 0.01 M Zn solution also improved the root and shoot length and seedling dry weight.


Copyright © 2018
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Optimizing zinc seed priming treatments for improving the germination and early seedling growth of wheat

Al-Mudaris AM, Jutzi SC. 1999. The influence of fertilizer-based seed priming treatment on emergence and seedling growth of Sorghum bicolor L. and Pennisteum glaucum L. in pot trials under greenhouse conditions. Journal of Agronomy and Crop Science 182, 135–141.

Aravind P, Prasad MNV. 2004. Carbonic anhydrase impairment in cadmium-treated, Ceratophyllum demersum L. (free floating freshwater macrophyte): toxicity reversal by zinc. Journal of Analytical Atomic Spectrophotometery 19, 52–57.

Aravind P, Prasad MNV. 2005a. Modulation of cadmium-induced oxidative stress in Ceratophyllum demersum by zinc involves ascorbate-glutathione cycle and glutathione metabolism. Plant Physiology and Biochemistry Journal 43, 107-116.

Aravind P, Prasad MNV. 2005b. Zinc mediated protection to the conformation of carbonic anhydrase in cadmium exposed Ceratophyllum demersum L. Plant Science Journal 169, 245–254.

Aravind P, Prasad MNV. 2005c. Cadmium-Zinc interactions in hydroponic system using Ceratophyllum demersum: adaptive plant ecophysiology, biochemistry and molecular toxicology. Brazilian Journal of Plant Physiology 17, 3–20.

Association of Official Seed Analyst (AOSA). 1990. Rules for testing seeds. Journal of Seed Technology 12, 1–112.

Auld DS. 2001. Zinc coordination sphere in biochemical zinc sites. Biometals 14, 271–313.

Bam RK, Kumaga FK, Ofori K, Asiedu EA. 2006. Germination, vigour and dehydrogenase activity of naturally aged rice (Oryza sativa L.) seeds soaked in potassium and phosphorous salts. Asian Journal of Plant Sciences 5, 948–955.

Basra SMA, Farooq M, Hafeez K, Ahmad N. 2004. Osmohardening: a new technique for rice seed invigoration. International Rice Research Institute Notes, 29, 80–81.

Basra SMA, Farooq M, Tabassum R, Ahmad N. 2005. Phisiological and biochemical aspects of seeds vigor enhancement treatments in fine rice (Oryza sativa L.). Seed Science Technology Journal 33, 623-628.

Basra SMA, Zia MN, Mehmood T, Afzal I, Khaliq A. 2002. Comparison of different invigoration techniques in wheat (Triticum aestivum L.) seeds. Pakistan Journal of Arid Agriculture 5, 11–6.

Bohnsack CW, Albert LS. 1977. Early effects of boron deficiency on indole acetic acid oxidase levels of squash root tips. Plant Physiology 59, 1047–1050.

Bradford KJ. 1986. Manipulation of seed water relations via osmotic priming to improve germination under stress conditions. Horticulture Science 21, 1105-1112.

Burgass RW, Powell AA. 1984. Evidence for repair processes in the invigoration of seeds by hydration. Annals of Applied Biology 53, 753– 757.

Cakmak I. 1997. Concentration of zinc and activity of copper/zinc superoxide dismutasein leaves of rye and wheat cultivars differing in sensitivity to zinc deficiency. Journal of Plant Physiology 151, 91–95.

Cakmak I. 2000. Role of zinc in protecting plant cells from reactive oxygen species. New Phytologist 146, 185–205.

Cakmak I. 2008. Enrichment of cereal grains with zinc: Agronomic or genetic biofortification? Plant Soil 302, 1-17.

Coolbear P, Francis A, Grieson G. 1984. The effect of low temperature pre-sowing treatment under the germination performance and membrane integrity of artificially aged tomato seeds. Journal of Experimental Botany 35, 1609-1617.

Ellis RA, Roberts EH. 1981. The quantification of ageing and survival in orthodox seeds. Seed Science Technology Journal 9, 373–409.

Farooq M, Basra SMA, Ahmad N. 2005b. Rice seed priming. International Rice Research Institute Notes 30, 45-48.

Farooq M, Basra SMA, Hafeez K, Ahmad N. 2005. Thermal hardening: a new seed vigor enhancement tool in rice. Journal of Integrative Plant Biology 47,187–193.

Farooq M, Basra SMA, Khalid M, Tabassum R, Mehmood T. 2006. Nutrient homeostasis, reserves metabolism and seedling vigor as affected by seed priming in coarse rice. Canadian Journal of Botany 84, 1196–1202.

Farooq M, Basra SMA, Wahid A, Khaliq A, Kobayashi N. 2009. Rice seed invigoration. In: Lichtfouse, E. (Ed.), Organic Farming, Pest Control and Remediation of Soil Pollutants, Sustainable Agriculture Reviews pp. 137–175.

Farooq M, Basra SMA, Wahid A. 2006c. Priming of field-sown rice seed enhances germination, seedling establishment, allometry and yield. Plant Growth Regulation 49, 285-294.

Farooq M, Wahid A, Siddique KHM. 2012. Micronutrient application through seed treatments – a review. Journal of Soil Science and Plant Nutrition 12, 125-142.

Fischer ES. 1997. Moderate magnesium deficiency affected chlorophyll content of bean plant. Photosynthetica 33, 385-390.

Kaya C, Higgs D. 2002. Response of tomato (Lycopersicone sculentum L.) cultivars to application of zinc when grown in sand culture at low zinc. Scientia Horticulture 93 53-64.

Ozturk L, Yazici MA, Yucel C, Torun A, Cekic C, Bagci A, Ozkan H, Braun HJ, Sayers Z, Cakmak I. 2006. Concentration and localization of zinc during seed development and germination in wheat. Plant Physiology 128, 144-152.

Pandey N, Pathak GC, Sharma CP. 2006. Zinc is critically required for pollen function and fertilisation in lentil. Journal of Trace Element in Medicine and Biology 20, 89-96.

Parer CA, Cantliffe DJ. 1994. Presowing seed priming. Horticultural Reviews 16, 109–141.

Prasd K, Saradhi PP, Sharmila P. 1999. Concerted action of antioxidant enzymes and curtailed growth under zinc toxicity in Brassica juncea. Environmental and experimental Botany 42, 1-10.

Rengel Z. 1995. Carbonic anhydrase activity in leaves of wheat genotypes differing in Zn efficiency. Journal of Plant Physiology 147, 251–256.

Rout GR, Das P. 2003. Effect of metal toxicity on plant growth and metabolism. Agronomie 23, 3–11.

Rowse HR. 1995. Drum priming a non-osmotic method of priming seeds. Seed Science Technology 24, 281-294.

Ruano A, Poschenrieder CH, Barcelo I. 1988. Growth and biomass partitioning in zinc toxic bush beans. Journal of Plant Nutrient 11, 577-588.

Simon EW. 1984. Early events in germination. In: Seed Physiology, Murray D.R. (Ed.). Germination and reserve mobilization. Academic press, Orlando, FL. Vol. 2, pp: 77–115.

Steel RGD, Torrie JH, Dickey DA. 1997. Principles and Procedures of Statistics: a Biometric Approach. 3rd Ed. McGraw Hill Book Co. Inc., New York. USA.

Teige M, Huchzermeyer B, Sehultz G. 1990. Inhibition of chloroplast ATPsenthease\ATPase is a primary effect of heavy metal toxicity in spinach plants. Biochemie und Physiologie der Pflanzen 186, 165-168.

Vitosh ML, Warncke DD, Lucas RE. 1994. Zinc determine of crop and soil. Michigan State University Extension.