Organochlorine pesticide residues inraw and cooked fish fillets

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Research Paper 01/09/2015
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Organochlorine pesticide residues inraw and cooked fish fillets

Abdelrahman Said Talab, Hala Elshahat Ghannam
J. Bio. Env. Sci.7( 3), 292-303, September 2015.
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The effects of microwave and halogen cooking on organochlorine pesticides residues of five commercial fish species fillets (Mullet, Nile tilapia, Blue tilapia, Mango tilapia and Bayad catfish) were assessed. Results showed that dichlorodiphenyltrichloroethane (DDT) and hexachlorocyclohexane(HCH) were the predominant contaminants in fish fillets. Besides, concentrations of endrin, endosulfan I, endosulfan II, ensulfan sulfate, endrin aldehyde, endrinketon and methoxychlor compound were below detectable limits in raw and cooked fish fillets. The high levels of HCHs were found in raw fillets of Bayad catfish, Mango tilapia, Blue tilapia, Mullet and Nile tilapia as (321, 225, 159, 143 and111 ng/g ww, respectively).Concerning the effect of thermal processing methods microwave and halogen caused significant (p<0.05) losses in the concentrations of most examined pesticides. The reduction rates in total organochlorine pesticides residues in microwave and halogen cooked samples were 71.89 and 79.80%, respectively. The highest losses were occurred in halogen cooked samples as follows: Bayad catfish (79.80%), followed by Mango tilapia (73.97%), Mullet (71.95%), Nile tilapia (68.40%), Blue tilapia (64.43%). Halogen cooking method was more effective than microwave in reducing the content of per-sistent organochlorine pesticides and it can be used to enhance the nutritional value of fish products and promote good health.


Abdallah MAM, Morsy FA. 2013. Persistent organchlorine pollutants and metals residues in sediment and freshwaterfish species cultured in a shallow lagoon, Egypt. Environmental Technology, 34, 2389-2399.

Abdou ES, Nagy AS, Sorour MA. 2012. Effect of chitosan and chitosan-nanoparticles as active coating on microbiological characteristics of fish fingers. International Journal of Applied Science and Technology 2(7), 158-169.

Barakat AO, Kim M, Qian Y, Wade TL. 2002. Organochlorine pesticides and PCB residues in sediments of Alexandria Harbour, Egypt. Baseline/Marine Pollution Bulletin 44, 1421-1434.

Bayen S, Barlow P, Lee HK, Obbard JP. 2005. Effect of cooking on the loss of persistent organic pollutants from salmon. Journal of Toxicology and Environmental Health,part A 68, 253-265.

Bocio A, Llobet JM, Domingo JL, Teixido A, Casas C. 2003. Polybrominateddiphenyl ethers (PBDEs) in food stuffs: Human exposure through the diet.Journal of Agricultural and Food Chemistry 51, 3191-3195.

Cin DA, Kroger M. 1982. Effects of various kitchen heat treatments, ultraviolet light, and Gamma irradiation on mirex Insecticide residues in fish.Journal of Food Science 47, 350-354.

EPA. 1992.Environmental Protection Agency. National study of chemical residues in fish; Office of Science and Technology (wh. 551): Washington, Dc. 1, EPA, 823-R92.008. A, B.

FAO/ WHO, 1998. Food standards program of codex Alimentarius Commission about pesticide residues in food maximum residue limits. Vol 2B, Rome.

Ghannam HE, Jahin HS, Gaber SE, Talab AS. 2014.Occurrence and distribution of chlorinated pesticide residues in water and fish of El-Bahr El-Pharaony drain El-Menoufia Governorate, Egypt. Pollution Research Journal 33(2), 251-257.

Holden AJ, Li Chen, Shaw IC. 2001. Thermal stability of organophosphorus pesticide triazophos and its relevance in the assessment of risk to the consumer of triazophos residues in food. Journal of Agricultural and Food Chemistry 49, 103-106.

Huan Z, Xu Z, Jiang W, Chen Z, Luo J. 2015. Effect of Chinese traditional cooking on eight pesticides residue during cowpea processing. Food Chemistry 170, 118-122.

Ikeura H, Hamasaki S, Tamaki M. 2013. Effects of ozone microbubble treatment on removal of residual pesticides and quality of persimmon leaves. Food Chemistry 138, 366-371.

IOC. 1993.Chlorinated biphenyls in open ocean waters: sampling, extraction, clean-up and instrumental determination. Manual and guides no. 27. Paris: Intergovernmental oceanographic commission, UNESSCO, 36.

Kelly BC, Ikonomou MG, Blair JD, Morin AE, Gobas FAPC. 2007. Food-web specific biomagnification of persistent organic pollutants. Science 317, 236-239.

Lee CK, Lee EH. 1985. Heat stability of organochlorine pesticide residues in loach. Bulletin of the National Fisheries University of Pusan 25(1), 85-90.

Mills PA, Bong BA, Kamps LR, Burke JA. 1972. Elution solvent system for florisil column cleans up in organochlorine pesticide residue analysis. Journal of the Association of Official Agricultural Chemists 55 (1), 39-43.

Mujawar S, Utture SC, Fonseca E, Matarrita J, Banerjee K. 2014.Validation of a GC-MS method for the estimation of dithiocarbamate fungicide residues and safety evaluation of mancozeb in fruits and vegetables. Food Chemistry 150, 175-181.

Pandelova M, Henkelmann B, Roots O, Simm M, Järv L, Benfenati E, Schramm KW. 2008. Levels of PCDD/F and dioxin-like PCB in Baltic fish of different age and gender. Chemosphere 71, 369-378.

Pandit GG, Sahu SK, Sharma S, Puranik VD. 2005. Distribution and fate of persistent organochlorine pesticides in coastal marine environment of Mumbai. Environment International 23, 240-243.

Said TO, Hamed MA. 2005. Distribution of chlorinated pesticides in surface water and fish of El Temsah and Bitter lakes, Suez Canal. Egypt. Journal of Aquatic Research 31(1), 202-212.

Salama AA, Mohamed MAM, Duval B, Potter TL, Levin RE. 1998. Polychlorinated Biphenyl Concentration in Raw and Cooked North Atlantic Bluefish (Pomatomussaltatrix) Fillets. Journal of Agricultural and Food Chemistry 46, 1359-1362.

Salem MA, EidAM. 2011. Depletion of polychlorinated biphenyl (PCBs) congeners in fresh Mugilcephelus and sardine fish after its exposure to different cooking treatments.World Applied Sciences Journal 15(12), 1645-1650.

Santerre CR, Ingram R, Lewis GW, Davies JT, Lane LG, Grodner RM, Wel CI, Bush PB, Xu DH, Shelton J, Alley EG, Hinshow JM. 2000. Organochlorine, organophosphates, and pyrthriods in channel catfish, rainbow trout, and red swamp crayfish from aquaculture facilities. Journal Food Science 65(2), 231-235.

Sharma  J,  Satya  S,  Kumar  V,  Tewary  DK. 2005.Dissipation of pesticides during bread making. Chemical Healthand Safety 12(1), 17-22.

Sidhu KS. 2003. Health benefits and potential risks related to consumption of fish or fish oils. Regulatory Toxicology and Pharmacology 38, 336-344.

Stefanelli P, Muccio AD, Ferrara F, Barbini DA, Generali T, Pelosi P, Amendola G, Vanni F, Mucci, SD, Ausili A. 2004.Estimation of intake of organochlorine pesticides and chlorobiphenlys through edible fishes from the Italian Adriatic Sea during 1997. Food Control 15, 27-38.

Storelli MM, Barone G, Garofalo R, Marcotrigiano GO. 2007. Metals and organochlorine compounds in eel (Anguilla anguilla) from the Lesina lagoon, Adriatic Sea (Italy). Food Chemistry 100, 1337-1341.

Svobodova Z, Zlabek V, Randak T, Machova J, Kolarova J, Hajslova J, Suchan P. 2003. Profiles of persistent organochlorine pollutants (POPs) in tissues of marketable common carp and in bottom sediments of selected ponds of South and West Bohemia.Journal ActaVeterinaria Brno 72, 295-309.

Takahashi S, Oshihoi T, Ramu K, Isobe T, Ohmori K, Kubodera T, Tanabe S. 2010.Organohalogen compounds in deep-sea fishes from the western North Pacific, off-Tohoku, Japan: Contamination status and bioaccumulation profiles. Marine Pollution Bulletin 60, 187-196.

Trotter WJ, Corneliussen PE, Laski RR, Vanelli JJ. 1989. Levels of polychlorinated biphenyls and pesticides in blue fish before and after cooking. Journal of the Association of Official Agricultural Chemists 72, 501-503.

UNEP/IOC/IAE. 1989. Determination of DDTs and PCBs in selected marine organisms by capillary column gas chromatography, reference methods for marine pollution study no. 40 Nairobi: United Nations Environment program, 18.

Witczak A. 2009.Effect of heat treatment on organochlorine pesticide residues in selected fish species.Polish Journal of Food and Nutrition Sciences 59(3), 231-235.

Yehouenou E, Pazou A, Lalèyè P, Boko M, Van Gestel CAM, Ahissou H, Akpona S, Van Hattum B, Swart K, Van Straalen NM. 2006. Contamination of fish by organochlorine pesticide residues in the Ouémé River catchment in the Republic of Bénin. Environment International 32, 594-599.

Zabik ME, Hoojjat P, Weaver CM. 1979. Polychlorinated biphenyls, dieldrin and DDT in lake trout cooked by broiling, roasting or microwave. The Bulletin of Environmental Contamination and Toxicology 21, 136-143.

Zabik ME, Zabik M, Booren A, Nettles M, Song J, Welch R, Humphrey H. 1995. Pesticide and total polychlorinated biphenyls in chinook salmon and carp harvested from the Great Lakes: effects of skin-on and skin-off processing and selected cooking methods. Journal of Agricultural and Food Chemistry 43, 993-1001.

Zabik ME, Zabik MJ. 1999. Polychlorinated biphenyls, polybrominated biphenyls and dioxin reduction during processing / cooking food.Impact of processing on food safety.Advances in experimental medicine and biology. Vol. 459 eds. L.S. Jakson, M.G.; Kinze, J.F. and Morgan; 213-231. New York. Plenum press.

Zhao L, Ge J, Liu F, Jiang N. 2014. Effects of storage and processing on residue levels of chlorpyrifos in soybeans. Food Chemistry 150, 182-186.