Welcome to International Network for Natural Sciences | INNSpub

Pathogenesis and molecular confirmation of Pseudomonas Syringae PV. Syrinage Isolates from peach and plum in Pakistan

Research Paper | May 1, 2016

| Download 2

Raees Ahmed, Muhammad Inam-ul-Haq, Muhammad Ashfaq, Zahid Akram

Key Words:

Int. J. Biosci.8( 5), 22-28, May 2016

DOI: http://dx.doi.org/10.12692/ijb/8.5.22-28


IJB 2016 [Generate Certificate]


Detailed survey of Peach and Plum orchards was done in 2014-2015 for the confirmation of bacterial canker disease in Pakistan and the isolates were isolated from diseased stone fruit tissue samples from 16 Orchard sites in Khyber Pakhtunkhwa (KPK), Pakistan. These isolates were tested for pathogenicity and the confirmation of Pseudomonas syringae pv. syringae was done using virD4 primer for the amplification of virD4 gene. All the 21 isolates from 24 isolates tested were moderately to highly pathogenic on peach seedlings while other 3 isolates showed no pathogenicity. During PCR assay of all the 24 isolates to amplify virD4 gene, the predicted 1453bp PCR products were obtained by 20 isolates and the 4 isolates were considered negative. The results highlighted a new threat in Pakistan, which must be further studied for its characterization and epidemiology for better management of bacterial canker of stone fruits in local environmental conditions.


Copyright © 2016
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Pathogenesis and molecular confirmation of Pseudomonas Syringae PV. Syrinage Isolates from peach and plum in Pakistan

Agrios G. 2005. Plant diseases caused by nematodes. Plant pathology 4, 565-97.

Bradbury J. 1986. Pseudomonas syringae pv. syringae. Guide to plant pathogenic bacteria 175-7.

Cameron HR. 1962. Diseases of deciduous fruit trees incited by Pseudomonas syringae van Hall: a review of the literature with additional data. In.: Corvallis: Agricultural Experiment Station, Oregon State University.

Fiori M, Cicconi L, Scortichini M. 2003. Bacterial canker of hazelnut (Corylus avellana L.) in Sardinia (Italy): occurrence of Pseudomonas syringae strains. In. Pseudomonas syringae and related pathogens. Springer 617-25.

Gardan L, Shafik H, Belouin S, Broch R, Grimont F, Grimont P. 1999. DNA relatedness among the pathovars of Pseudomonas syringae and description of Pseudomonas tremae sp. nov. and Pseudomonas cannabina sp. nov.(ex Sutic and Dowson 1959). International Journal of Systematic Bacteriology 49, 469-78. http://dx.doi.org/10.1099/00207713-49-2-469

Hetherington S, Wales NS. 2005. Bacterial canker of stone fruit.

Ivanova L. 2007. First occurrence of apricot blast disease caused by Psеudomonas syringae in the north-eastern part of Bulgaria. Proceedings of the I Balkan Symposium on Fruit Growing 149-52.

Karimi-Kurdistani G, Harighi B. 2008. Phenotypic and molecular properties of Pseudomonas syringae pv. syringae the causal agent of bacterial canker of stone fruit trees in Kurdistan province. Journal of Plant Pathology 81-6. http://dx.doi.org/10.4454/jpp.v90i1.595

Khan AA, Furuya N, Matsumoto M, Matsuyama N. 2000. Differentiation of phytopathogenic Pseudomonas and Xanthomonas pathovars and strains by PCR analysis for DNA topoisomerase genes. Journal of the Faculty of Agriculture, Kyushu University 45, 1-6.

Kotan R, Şahin F. 2002. First record of bacterial canker caused by Pseudomonas syringae pv. syringae, on apricot trees in Turkey. Plant pathology 51, 798. http://dx.doi.org/10.1046/j.1365-3059.2002.00768.x

Mohammadi M, Ghasemi A, Rahimian H. 2010. Phenotypic Characterization of Iranian Strains of Pseudomonas syringae pv. syringae van Hall, the Causal Agent of BacterialCanker Disease of Stone Fruit Trees. Journal of Agricultural Science and Technology 3, 51-65.

Scortichini M, Marchesi U, Dettori M, Rossi M. 2003. Genetic diversity, presence of the syrB gene, host preference and virulence of Pseudomonas syringae pv. syringae strains from woody and herbaceous host plants. Plant pathology 52, 277-86. http://dx.doi.org/10.1046/j.13653059.2003.00860.x

Severin V, Stancescu C, Foad I. 1986. Bacterial canker of peach in Romania. Analele-Institutului-de-Cercetari-pentru-Protectia-Plantelor 19, 55-58. cf CABI Publishing, UK, 378 p.

Sundin G, Mayfield C, Zhao Y, Gunasekera T, Foster G, Ullrich M. 2004. Complete nucleotide sequence and analysis of pPSR1 (72,601 bp), a pPT23A-family plasmid from Pseudomonas syringae pv. syringae A2. Molecular Genetics and Genomics 270, 462-76. http://dx.doi.org/10.1007/s00438-003-0945-9

Vicente JG, Roberts SJ. 2007. Discrimination of Pseudomonas syringae isolates from sweet and wild cherry  using  rep-PCR.  European  journal  of  plant pathology 117, 383-92. http://dx.doi.org/10.1007/s10658-007-9107-y

Young J, Saddler G, Takikawa Y, De Boer S, Vauterin L, Gardan L, Gvozdyak R, Stead D. 1996. Names of plant pathogenic bacteria 1864-1995. Review of Plant Pathology 75, 721-63.