PCR base identification of Shigella in children with diarrhea at Noshki, Balochistan
Paper Details
PCR base identification of Shigella in children with diarrhea at Noshki, Balochistan
Abstract
A total of 200 samples were collected out of which 76% were found Shigella negative and 24% were found positive. The district head quarter hospital Noshki, different basic health unit rural and basic health unit urban results showed that, district head quarter hospital Noshki have Shigella 11%, basic health unit urban has Shigella 07% and basic health unit rural have Shigella 06%. However, in our study sex wise ratio showed that males were (13%) more affected as compared to female (11%). Microbial infection leading to Shigella diarrhoea is a major contributor to neonatal deaths in developing world. Whereas age wise distribution showed 9% in one year, 7% in two years, 6% in three years, 1% in four years and 1% in five years of age. The culture media composition is very important for bacterial growth. Most of the previous studies on Shigella development and growth have been performed under optimal conditions. Shigella best growth was recorded on Salmonella Shigella Agar and Xylose Lysine Deoxycholase Agar. In this study bacteria grow on pH ranges from 6-8 and temperature of 04-47ºC. Shigella showed sensitivity to most of the antibiotics classes, whereas multi drug resistance against Ampicillin, Penicillin, Chloramphenicol, Tetracycline classes. Apolymerase chain reaction indicates that the rapidity and high specificity of the Shigella rfp B gen -specific assays provide optimal efficiency without the need for additional hybridization. While conformation through PCR sample shows clear of 211bp of Shigella rfp B gene.
Ahmed K, Abdul RS, Farah RS. 2003. A etiology of shigellosis in northern Pakistan. Journal Health Popul Nutr 21(1), 32-9.
Cowan, Steel. 1993. Manual for the identification of medical bacteria, 3rd edition, Cambridge University press.
Elliott EJ. 2007. Acute gastroenteritis. British Medical Journal 33(4), 35-40.
Getnet AG. 2014. Prevalence and Antimicrobial Susceptibility Patterns of Shigella and Salmonella Species among Patients with Diarrhea Attending Gondar Town Health Institutions, Northwest Ethiopia. Science Journal Public Health 5(2), 469-475.
Ghosh M, Wahi S, Kumar M, Ganguli A. 2007. Prevalence of Enterotoxigenic Staphylococcus aureus and Shigella spp. in some raw street vended Indian foods. International Journal of Environmental Health Research 17(2), 151-156.
Hiranrattana A, Mekmullica J, Chatsuwan T, Pancharoen C, Thisyakorn U. 2005. “Childhood shigellosis at King Chulalongkorn Memorial Hospital, Bangkok, Thailand: a 5-year review (1996-2000),” South east Asian Journal of Tropical medicine and Public Health, 36(3), 683-685.
Levine MM, Caplan ES, Waterman D, Hornick R, Snyder MJ. 1977. Diarrhea caused by Escherichia coli that produce only heat-stable enterotoxin. Journal of Infection and Immunity 1(7), 78-82.
Levine MM, Du PHL, Hornick RB. 1989. Inoculum size in shigellosis and implication for expected mode of transmission. Journal of Infectious Disease 15(9), 1126-1128.
Niyogi SK. 2005. Increasing antimicrobial resistance-an emerging problem in the treatment of shigellosis. Journal of microbiology 43(2), 133-143.
Oyofo BA, Lesmana M, & Subekti D. 2002. “Surveillance of bacterial pathogens of diarrhea disease in Indonesia, “Diagnostic Microbiology and Infectious Disease 44(3), 227-234.
Singh KKB, Ojha SC, Deris ZZ, Rahman RA. 2011. “A 9-year study of shigellosis in North east Malaysia antimicrobial susceptibility and shifting species dominance, “Journal of Public Health 19(3), 231-236.
Steins LH, Valentiner BP, Grewal HM, Gaastra W. 2003. Development and evaluation of genotypic assays for the deletion and characterization of Enterotoxigenic E. coli. Journal of Diagnosis of Microbial Infections 4(5), 97-105.
Sur DT, Ramamurthy JD, Bhattacharya SK. 2004. “Shigellosis challenges & management issues,” The Indian Journal of Medical Research 120(5), 454-457.
Timaeus IM, Lush L. 1995. Intra-urban differentials in child health. Health Transition Reviews 5(1), 163-190.
Vogt RL, Dippold L. 2005. Escherichia coli O157:H7 outbreak associated with consumed ground June-July 2002. Public Health Reports 120(2), 174-8.
Aziz Ullah, Muhammad Kamran Taj, Zafar Ahmed, Zahoor Ahmed, Imran Taj, Zohra Samreen, Nargis Taj, Mahrukh Naseem, Azmat Ullah, Abdul Rehman Sherani, Ghulam Muhammad, Qazi Dad Muhammad Anwar, Saima Azam, 2019. PCR base identification of Shigella in children with diarrhea at Noshki, Balochistan. J. Biodiv. Environ. Sci., 14(5), 37-44.
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