PCR base identification of Shigella in children with diarrhea at Noshki, Balochistan

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Research Paper 01/05/2019
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PCR base identification of Shigella in children with diarrhea at Noshki, Balochistan

Aziz Ullah, Muhammad Kamran Taj, Zafar Ahmed, Zahoor Ahmed, Imran Taj, Zohra Samreen, Nargis Taj, Mahrukh Naseem, Azmat Ullah, Abdul Rehman Sherani, Ghulam Muhammad, Qazi Dad Muhammad Anwar, Saima Azam
J. Bio. Env. Sci.14( 5), 37-44, May 2019.
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A total of 200 samples were collected out of which 76% were found Shigella negative and 24% were found positive. The district head quarter hospital Noshki, different basic health unit rural and basic health unit urban results showed that, district head quarter hospital Noshki have Shigella 11%, basic health unit urban has Shigella 07% and basic health unit rural have Shigella 06%. However, in our study sex wise ratio showed that males were (13%) more affected as compared to female (11%). Microbial infection leading to Shigella diarrhoea is a major contributor to neonatal deaths in developing world. Whereas age wise distribution showed 9% in one year, 7% in two years, 6% in three years, 1% in four years and 1% in five years of age. The culture media composition is very important for bacterial growth. Most of the previous studies on Shigella development and growth have been performed under optimal conditions. Shigella best growth was recorded on Salmonella Shigella Agar and Xylose Lysine Deoxycholase Agar. In this study bacteria grow on pH ranges from 6-8 and temperature of 04-47ºC. Shigella showed sensitivity to most of the antibiotics classes, whereas multi drug resistance against Ampicillin, Penicillin, Chloramphenicol, Tetracycline classes. Apolymerase chain reaction indicates that the rapidity and high specificity of the Shigella rfp B gen -specific assays provide optimal efficiency without the need for additional hybridization. While conformation through PCR sample shows clear of 211bp of Shigella rfp B gene.


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