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Research Paper | March 1, 2022

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PCR based detection of mecA gene of Staphylococcus aureus isolated from surgical wound patients in Quetta

Asima Safdar, Mumtaz Ali Sanjrani, Zafar Ahmed, Muhammad Alam Mengal, Fatima Abdul Majeed, Saima Azam, Fahmeeda Rehman

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Int. J. Biosci.20(3), 211-217, March 2022

DOI: http://dx.doi.org/10.12692/ijb/20.3.211-217

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Abstract

Staphylococcus aureus is an acquired hospital infection and which has been shown to develop resistance against several antibiotics. Polymerase Chain Reaction (PCR) was performed to categorize bacterial antibiotic-resistant. The present study aimed to examine S. aureus in pus samples through using Polymerase Chain Reaction and study the resistance of the bacterial isolates against certain antibiotics. A total of (n=100) pus samples were obtained from surgical wound patients through sterile cotton swabs.  The samples were immediately cultured, and positive samples were tested for gram staining, biochemical test, and antibiotic susceptibility. Our study showed that 58(58%) of the surgical patients were found positive for S.aureus and 42 (42%) were showed negative. This current study represents the S.aureus bimolecular identification and screening test of strain methicillin-resistant bacteria from the pus of surgical wounds of different hospitals from Quetta Balochistan. From hundred samples of 58 isolates of Staphylococcus aureus were confirmed through biochemical and PCR-based techniques. This current study reveals the S.aureus resistance variability against different antibiotics.

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PCR based detection of mecA gene of Staphylococcus aureus isolated from surgical wound patients in Quetta

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