Int. J. Biosci.14(2), 172-182, February 2019
Arthritis is a painful muscoskeletal disorder of unknown etiology affecting a large population globally. Non-steroidal antiinflammatory drugs have been used for long but have side effects like gastric ulcer, gastric bleeding, leucopenia etc. Bellamya bengalensis is a freshwater mollusc found abundantly of Viviparidae family was used to treat various ailments like conjunctivitis, arthritis etc. The present study aimed to show the attenuation of inflammation by phosphate buffer saline extract of Bellamya bengalensis Lam (PBB) in Freund’s Complete adjuvant (FCA) induced arthritis model. The arthritis was induced in Wistar rats using FCA. Changes in body weight, paw volume, ankle diameter, arthritic score were studied as parameters of chronic inflammation. Serum cytokine profile and tissue antioxidant profile of the paw was studied at the end of the study. Radiology of the hindlegs and the histopathology of the ankle joints were also studied. The PBB treated group of rats showed normal gain in body weight with reduction in paw volume by 90.23% and 96.27% (p<0.01) at 200mg/kg and 400mg/kg when compared with FCA induced control. The proinflammatory cytokine was reduced by 56.79% in serum in PBB 400 mg/kg and the altered antioxidant parameters in inflamed rat paw were restored back to normal when treated with PBB extracts. Histopathology results revealed fewer inflammatory lesions in PBB treated rats when compared to adjuvant induced control rats. In conclusion, PBB at a dose of 200mg/kg and 400mg/kg, orally for 28 days showed significant reduction in chronic inflammation which may be through TNF-α inhibitory pathway.
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