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Research Paper | August 20, 2022

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Phenolic characterization and lipid lowering properties of powder fractions and ethanolic extract of Adansonia digitata pulp.

Linda Stella Mbassi, Elie Baudelaire Djantou, Josiane Therese Metsagang Ngatchic, Amadou Dicko, Nicolas Yanou Njintang

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Int. J. Biosci.21(2), 363-377, August 2022

DOI: http://dx.doi.org/10.12692/ijb/21.2.363-377

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Abstract

This study aimed to evaluate the effect of controlled differential sieving treatment on the phenolic content and antihyperlipidemic activity of A. digitata pulp and compared it to the effect of ethanolic extract. For this purpose, powders with particle sizes ranging from 20 to 100 µm and above were produced and analyzed. As a first step, we identified and quantified the phenolic compounds present in the samples by chromatography in liquid phase coupled to a UV detector and mass spectrometry (LC-MS). Then, we evaluated the antihyperlipidemic activity of the different fractions in adult male rats at a dose of 250 mg/kg and compared them with those of unsieved powder and the lyophilized ethanolic extract. LC-MS analysis identified four compounds (quercetin, rutin, ferrulic acid and cafeic acid) in the different sample of A. digitata pulp powder. The granulometric class <50μm showed the best concentration, higher than those of the other granulometric class and crude powder. This fraction also showed content of phenolic compounds similar to those of ethanolic extract except cafeic acid which was more concentrated in the ethanolic extract. The antihyperlipidemic activity were significantly influenced (P <0.05) by particle sizes. The best activity was obtained from the <50μm µm fraction which was significantly (P <0.05) higher than the other fractions and the unsieved powder to improve the lipid parameters (TC, LDL-C, HDL-C and TG), transaminases (ALAT and ASAT) and creatinine. It showed similar activity to the ethanolic extract with the exception of LDL-C which is lower in this fraction. Controlled differential sieving process can be an alternative to ethanol extraction and may be an option to improve the content of phenolic compounds and antihyperlipidemic activity of plant powders.

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Phenolic characterization and lipid lowering properties of powder fractions and ethanolic extract of Adansonia digitata pulp.

Acham I, Eke M, Edah J. 2020. Physicochemical, microbiological and sensory quality of the juice mixture produced from watermelon fruit pulp and baobab fruit pulp powder. Croatian Journal of Food Science and Technology 12(1), 48-55. https://doi.org/10.17508/CJFST.2020.12.1.07

Alrasheid A, Ahmed SA, Eltilib SH, Alnour MI, Widdatallh MO, Yassin LF, Ayoub SMH. 2019. L’effet du mélange d’extraits d’Adansonia digitata soudanaise et de Tamarindus indica sur leurs activités antioxydantes, anti-inflammatoires et antimicrobiennes. Journal of Pharmacognosy and Phytotherapy 11(2), 28-34. https://doi.org/10.5897/JPP2019.0537

Ameer K, Shahbaz HM, Kwon JH. 2017. Green extraction methods for polyphenols from plant matrices and their byproducts: a review. Comprehensive Reviews in Food Science and Food Safety 16, 295– 31. https://doi.org/10.1111/1541-4337.12253

Baudelaire ED. 2013. Comminution and controlled differential screening method for the dry extraction of natural active principles. Google patent WO2013057379A1.

Becker L, Zaiter A, Petit J, Zimmer D, Karam MC, Baudelaire E, Dicko A. 2016. Improvement of antioxidant activity and polyphenol content of Hypericum perforatum and Achillea millefolium powders using successive grinding and sieving. Industrial Crops Products 87, 116–123. https://doi.org/10.1016/j.indcrop.2016.04.036

Bourely J. 1982. Observation on the dosage of cottonseed oil. Cotton and Tropical Fibers 27, 183-196.

Cujic´ N, Šavikin K, Jankovic´ T, Pljevljakušic´ D, Zdunic´ G, Ibric´ S. 2015. Optimization of polyphenols extraction from dried chokeberry using maceration. Food Chemistry 194, 135–142. https://doi.org/10.1016/j.foodchem.2015.08.008

Deli M, Nguimbou RM, Baudelaire E, Yanou N, Scher J, Mbofung CM. 2020. Effect of controlled differential sieving processing on micronutrient contents and in vivo antioxidant activities of H. sabdariffa L. powder. Food science and biotechnology 29, 1741–1753. https://doi.org/10.1002/fsn3.1022

Dufour DR, Lott JA, Nolte FS, Gretch DR, Koff RS, Seeff LB. 2000. Diagnosis and monitoring of hepatic injury I. Performance characteristics of laboratory tests. Clinical chemistry 46(12), 2027-2049.  https://doi.org/10.1093/clinchem/46.12.2027

Eman M, Amany YM. 2008. Some studies on acrylamide intoxication in male albino rats. Egyptain journal of comparative pathology and clinical pathology 21(4).

Fossati P, Prencipe L. 1982. Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide. Clinical chemistry 28(10), 2077–2080.

Friedewald WT, Levy RI, Fredrickson DS. 1972. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clinical chemistry 18(6), 499–502.

Gordon T, Castelli WP, Hjortland MC, Kannel WB, Dawber TR. 1977. High density lipoprotein as a protective factor against coronary heart disease. The Framingham Study. The American journal of medicine 62(5), 707–714. https://doi.org/10.1016/0002-9343(77)90874-

Hamlat N, Neggazi S, Benazzo Y, Kacimi G, Chaid S, Aouichat-Bouguerra S. 2008. Hyperlipidic diet and atherosclerosis process in Rattus norvegicus. Science and Technology 27, 49-56.

Henry RJ. 1974. Clinical chemistry, principles and technics. 2nd Edition, Harper and Row 525.

Ikeda I, Yamahira T, Kato M, Ishikawa A. 2010. The polyphenols in black tea decrease the micellar solubility of cholesterol in vitro and the intestinal absorption of cholesterol in rats. Journal of Agricultural and Food Chemistry 58(15), 8591-8595. https://doi.org/10.1021/jf1015285

Jodynis-Liebert J, Nowicki M, Murias M, Adamska T, Ewertowska M, Kujawska M, Pernak J. 2010. Cytotoxicity, acute and subchronic toxicity of ionic liquid, didecyldimethylammonium saccharinate, in rats. Regulatory Toxicology and Pharmacology 57(2-3), 266-273. https://doi.org/10.1016/j.yrtph.2010.03.006

Kitamura S. 2006. Transport of flavonoids: from cytosolic synthesis to vacuolar accumulation. In The Science of Flavonoids. 123-146. https://doi.org/10.1007/978-0-387-28822-2_5

Laviolle B, Annane D, Fougerou C, Bellissant  E. 2012. Gluco-and mineralocorticoid biological effects of a 7-day treatment with low doses of hydrocortisone and fludrocortisone in septic shock. Intensive care medicine 38(8), 1306-1314.

Leonardo LP, Feitoza GS, Costa JG. 2013. Development and validation of a HPLC method for the quantification of three flavonoids in a crude extract of Dimorphandra gardneriana. Revista Brasileira de Farmacognosia 23(1), 58-64.

Levine GN, Keaney JF, Vita JA. 1995. Cholesterol reduction in cardiovascular disease. Clinical benefits and possible mechanisms. The New England journal of medicine 332(8), 512–521. https://doi.org/10.1056/NEJM199502233320807

Miron TL, Plaza M, Bahrim G, Ibáñez E, Herrero M. 2011. Chemical composition of bioactive pressurized extracts of Romanian aromatic plants. Journal of Chromatography A 1218(30), 4918-4927. https://doi.org/10.1016/j.chroma.2010.11.055

Monroy YM, Rodriguesb RF, Sartorattob A, Cabral FA. 2016. Optimization of the extraction of phenolic compounds from purplecorn cob (Zea mays L.) by sequential extraction using supercriticalcarbon dioxide, ethanol and water as solvents. Journal of Supercritical Fluids 116, 10–19. 10.1016/j.supflu.2016.04.011

Muhammad IU, Jarumi IK, Alhassan AJ, Wudil AM, Dangambo MA. 2016. Acute toxicity and hypoglycemic activity of aqueous fruit pulp extract of Adansonia digitata L (Afpead) on alloxan induced diabetic rats. Journal of Advances in Medical and Pharmaceutical Sciences 6(3), 1-6.

Naito M, Kuzuya F, Asai K, Yoshimine N. 1984. Ineffectiveness of Ca2+ antagonist’s nicardipine and diltiazem on experimental atherosclerosis in cholesterol-fed rabbits. Angiology 35, 622627.  https://doi.org/10.5551/jat1973.14.3_647

Ngatchic J, Fomekong C, Baudelaire E, Njintang, N. 2020. Antioxidant and Antihyperlipidemic Properties of Different Granulometric Classes of Adansonia digitata Pulp Powder. Pakistan Journal of Nutrition 19, 393-403. 10.3923/pjn.2020.393.403

Oghbaei M, Prakash J. 2016. Effect of primary processing of cereals and legumes on its nutritional quality: A comprehensive review. Cogent Food & Agriculture 2(1), 1136015. https://doi.org/10.1080/23311932.2015.1136015

Ota A, Abramovič H, Abram V, Ulrih NP. 2011. Interactions of p-coumaric, caffeic and ferulic acids and their styrenes with model lipid membranes. Food Chemistry 125(4), 1256-1261. https://doi.org/10.1016/j.foodchem.2010.10.054

Pratt D, Kaplan MM. 2000. Evaluation of abnormal liver-enzyme results in asymptomatic patients. New England Journal of Medicine. 342, 1266-1271. https://doi.org/10.1056/NEJM200004273421707

Reitman S, Frankel S. 1957.  American. Journal clinicals pathology 28, 56–60.

Sakr AR, Mahran HA, Nofal AE. 2011. Effect of selenium on carbimazole-induced testicular damage and oxidative stress in albino rats. Journal of Trace Elements in Medicine and Biology 25(1), 59-66. https://doi.org/10.1016/j.jtemb.2010.07.002

Shi Y, Guo R, Wang X, Yuan D, Zhang S, Wang J, Wang C. 2014. The regulation of alfalfa saponin extract on key genes involved in hepatic cholesterol metabolism. PloS one 9(2), e88282. https://doi.org/10.1371/journal.pone.0088282

Zaiter A, Becker L, Petit J, Zimmer D, Karam MC, Baudelaire É, Dicko A. 2016. Antioxidant and antiacetylcholinesterase activities of different granulometric classes of Salix alba bark powders. Powder Technology 301, 649-656. https://doi.org/10.1016/j.powtec.2016.07.014

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