Phenotypic and genotypic screening of multi-drug resistant ESBL producing Escherichia coli and Klebsiella pneumoniae from burn wound infection

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Phenotypic and genotypic screening of multi-drug resistant ESBL producing Escherichia coli and Klebsiella pneumoniae from burn wound infection

Sohana Al Sanjee, Azizul Haque, Sanchita Banerjee, Md. Shihabul Hoque, Md. Moniruzzaman, Keshob Chandra Das, Md. Ekramul Karim
Int. J. Biosci.16( 5), 145-155, May 2020.
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As burn cases are very frequent in Bangladesh, the aim of the present study was to screen the multidrug resistant extended-spectrum-β-lactamase (ESBL) producing Escherichia coli and Klebsiella pneumoniae from burn wound infection. E. coli and K. pneumoniae were isolated on MacConkey agar and Eosin Methylene Blue (EMB) agar and identified by their colony characteristics and biochemical tests. Antimicrobial susceptibility of the isolates was performed by Kirby-Bauer disc diffusion method. After phenotypic ESBL confirmation, isolates were checked for the presence of ESBL genes by polymerase chain reaction (PCR). Among the isolates, K. pneumoniae (62.5%, n = 10) was highly prevalent one followed by E. coli (37.5%, n = 6). E. coli were sensitive to nitrofurantoin (66.67%) while K. pneumoniae were sensitive to amikacin (40%) and gentamicin (40%). Both of the isolates showed complete resistance (100%) to cefotaxime (30 µg), ceftazidime (30 µg) and cefoxitin (30 µg). MAR index was in the range of 0.69-1.0 for all the isolates except, one. All the isolates (100%, n = 16) were phenotypically positive for ESBL production. In PCR analysis, dominant ESBL class was found as blaTEM (87.5%, n = 14) followed by blaSHV (37.5%, n = 6). The co-existence of blaTEM and blaSHV in K. pneumoniae was also observed (50%, n = 5). However, none of the E. coli isolates harbored blaSHV gene. The findings of the present study showed that all the isolates were multidrug resistant and ESBL positive, which might be a serious threat to the clinical management of burn wound infection.


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